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Anticancer Effects of the Extracts of Adonis multiflora
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  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.561-567
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.561
 Title & Authors
Anticancer Effects of the Extracts of Adonis multiflora
Han, Hyo-Sang;
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This study aims to evaluate the antitumor effect of Adonis multiflora, one of the plants in the Ranunculaceae, on mice to which hepatoma cells were transplanted and to suggest its possibility as a candidate natural substance to replace antitumor drugs. We performed the MTT assay to assess the extract had a decrease in the growth rate of hepatoma cells depending on concentration. In particular, 100 ㎍/㎖ of the extract showed 40% of growth retardation rate. We assessed the autophagy activity to identify the inhibitory autophagy mechanism of tumor cells in the extract. This proved that the activity increases more as the concentration of the extract is higher. We conducted the Western blot test to confirmed the expression of two proteins LC3 and p62. The expression of p62 was in inverse proportion to the concentration of the extract whereas LC3-Ⅱ increased more as the concentration of the extract was higher. This showed that an increase in the autophagy relies on the conentration of the extract. We performed a test to discover the influence of the extracts on hepatoma cells transplanted to mice. The test proved that the extract triggers a significant decrease in the growth rate of tumor cells. Compared to the start of the test, the size of tumor cells with 50, 100 and 200 ㎎/㎏ of the extract respectively increased by 4, 3.7 and 3.5 times whereas in the controlling group by 6.3 times. The size of tumor cells in benign tumor controlling group increased by 3.1 times. This showed a significant decrease in the growth rate of tumor cells compared to the controlling group. We carried out the experiment of influence of the extract on the expression of two proteins LC3 and p62 in the tumor tissue transplanted into mice. The experiment showed that LC3-II increases more as the concentration of the extract is higher. However, there was a rapid decrease in p62 with 200 ㎎/㎏ of the extract compared to the controlling group. In this study, we proved that the autophagy activity of Adonis multiflora extract inhibits the growth of hepatoma cells by in vitro and in vivo experiments. In conclusion, the inhibitory autophagy mechanism of tumor cells in the extract can be used as a new treatment of antitumor.
Adonis multiflora Nishikawa et Ito extract;SK-Hep-1;MTT assay;Autophagy activity assay;Xenograft;Western blot assay;
 Cited by
Bjorkoy, G., T. Lamark, A. Brech, H. Outzen, M. Perander, A. Overvatn, H. Stenmark and T. Johansen. 2005. p62/SQSTM1 forms protein aggregates degraded by autophagy and has a protective effect on huntingtin-induced cell death. J. Cell. Biol. 171(4):603-614. crossref(new window)

Chen, N. and V. Karantza. 2011. Autophagy as a therapeutic target in cancer. Cancer Biol. Ther. 11(2):157-168. crossref(new window)

Corcella, EA., P. Puustinen and M. Jaattela. 2009. Apoptosis and autophagy: Targeting autopahgy signalling in cancer cells-‘trick or treats’? FEBS J. 276(21):6084-6096. crossref(new window)

Cronquist, A. 1981. An intergrated system of classification of flowering plants. Columbia Univ. Press, New York (USA). pp. 116-127.

Cuervo, AM. 2004. Autophagy: in sickness and in health. Trends Cell Biol. 14(2):70-77. crossref(new window)

Eisenberg-Lerner, A., S. Bialik, HU. Simon and A. Kimchi. 2009. Life and death partners:apoptisis, autophagy and the cross-talk between them. Cell Death Differ. 16(7):966-975. crossref(new window)

Ham, Y.A., H.J. Choi, S.H. Kim, M.J. Chung and S.S. Ham. 2009. Antimutagenic and antitumor effects of Adenophora triphylla extracts. J. Korean Soc. Food Sci. Nutr. 38(1):25-31 (in Korean). crossref(new window)

Hoffman, MH. 1998. Ecogeographical differentiation patterns in Adonis sect. Consiligo (Ranunculaceae). PI. Syst. Evol. 211:43-56. crossref(new window)

Im, RJ. 1996. Flora Coreana 2. The Science and Technology Publishing House, Pyongyang, DPRK. pp. 235-237.

Iman, M., J. Chrtek and Z. Slavikova. 2002. Effect of temperature and photosynthetic photon flux density on the photosynthesis of Adonis amurensis. Kor. J. Hort. Sci. Technol. 20 (Suppl. I):970.

Jun, N.J., S.C Kim, E.Y. Song, K.C. Jang, D.S Lee and S.K. Cho. 2014. Isolation of anticancer compounds from Peucedanum japonicum Thunb. roots. Korean J. Plant Res. 27(3):215-222 (in Korean). crossref(new window)

Kabeya, Y., N. Mizushima, T. Ueno, A. Yamamoto, T. Kirisako, T. Noda, E. Kominami, Y. Ohsumi and T. Yoshimori. 2000. LC3, a mammalian homologue of yeast Apg8p, is localized in autophagosome membranes after processing. EMBO J. 19(21):5720-5728. crossref(new window)

Kang, C. and L. Avery. 2008. Tobe or not to be, the level of autophagy is the question:dual roles of autophagy in the survival response to starvation. Autophagy 4(1):82-84. crossref(new window)

Kimmelman, AC. 2011. The dynamic nature of autophagy in cancer. Genes Dev. 25(19):1999-2010. crossref(new window)

Lee, C.H., S. Lee, Y. Suh, S.H. Yeau and N.S. Lee. 2003. A morphological reexamination on the genus Adonis L. sensu lato (Ranunculaceae) in Korea. Korean Journal of Plant Taxonomy 33(4):435-454 (in Korean).

Lee, H.B., H.J. Kim, M.S. Chong, , H.E. Cho, Y.H. Choi, K.S. Lim and K.N. Lee. 2008. Physiological activity of extracts from mixed culture of medical herbs and mycelia of Tricholoma matsutake and Cordyceps militaris by fermentation. Kor. J. Herbology 23(1):1-8 (in Korean).

Lee, J.W., G.H. Park, H.J. Eo, H.M. Song, M.K Kim, M.J. Kwon, J.S. Koo4, J.R. Lee, M.H. Lee and J.B. Jeong. 2015. Anti-cancer activity of the flower bud of Sophora japonica L. through upregulating activating transcription factor 3 in human colorectal cancer cells. Korean J. Plant Res. 28(3): 297-304 (in Korean). crossref(new window)

Lee, Y.N. 2006. New Flora of Korea I. Kyohaksa, Seoul, Korea. p. 975.

Mabberley, DJ. 1990. The Plant Book. Cambridge University Press, New York (USA). pp. 99-102.

Maiuri, MC., E. Zalckvar, A. Kimchi and G. Kroemer. 2007. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat. Rev. Mol. Cell Biol. 8(9):714-725.

Matsuyama, R., S. Reddy and T.J. Smith. 2006. Why do patients choose chemotherapy near the end of life? A review of the perspective of those facing death from cancer. J. Clin. Oncol. 24(21):3490-3496. crossref(new window)

Meusel, H., E. Jaeger and E. Weinert. 1965. Vergleichende Chorologie der zentraleuropaischen Flora. G. Fischer. Stuttgart, Germany. pp. 557-583.

Mizushima, N., B. Levine, A.M. Cuervo and D.J. Klionsky. 2008. Autophagy fights disease through cellular self-digestion. Nature 451(7182):1069-1075. crossref(new window)

Ohwi, T. 1984. Flora of Japan (in English). National Science museum. Tokyo, Japan. p. 450.

Park, C.W. 2007. Adonis: In The Genera of Vascular Plants of Korea. Park, C.W. (ed.), Academy Publishing Co., Seoul, Korea. pp. 184-185.

Schulze-Osthoff, K., D. Ferrari, M. Los, S. Wesselborg and M.E. Peter. 1998. Apoptosis signaling by death receptors. Eur. J. Biochem. 254(3):439-459. crossref(new window)

Shin, J.H, H.Y. Jang, J.S Chung, K.H Cho, K.E. Hwang, S.Y. Kim, H.J. Kim, S.Y. Lee, M.K. Lee, S.A. Park, S.R. Moon, K.K. Lee, H.J. Jo and S.H. Yang. 2010. Induction of autophagy by low dose of cisplatin in H460 lung cancer cells. Tuberc. Respir. Dis. 69:16-23 (in Korean). crossref(new window)

Tamura, M. 1990. A new classification of the family Ranunculaceae 1. Acta Phytotax. Geobot. 41(1-3):93-101.

Weinberg, R.A. 1994. Oncogenes and tumor suppressor genes. CA Cancer J. C lin. 44 (3):160-170. crossref(new window)