Advanced SearchSearch Tips
Anti-inflammatory Effects on 80% Ethanol Extract and Ethyl Acetate Fraction of Acrosorium yendoi Yamada in Murine Macrophage RAW 264.7 Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.574-581
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.574
 Title & Authors
Anti-inflammatory Effects on 80% Ethanol Extract and Ethyl Acetate Fraction of Acrosorium yendoi Yamada in Murine Macrophage RAW 264.7 Cells
Ko, Chang-Sik; Hyun, Woo-Cheol; Kim, Ji-hyun; Ko, Yeong-Jong; Song, Sang Mok; Ko, Mi-Hee; Lee, Jong-Chul; Kim, Chang-Sook; Yoon, Weon-Jong;
  PDF(new window)
This study describes a preliminary evaluation of the anti-inflammatory activity of Acrosorium yendoi Yamada extracts. A. yendoi Yamada was extracted using 80% ethanol and then fractionated sequentially with n-hexane, ethyl acetate and butanol. To screen for anti-inflammatory agents effectively, we first examined the inhibitory effect of 80% EtOH extract and solvent fractions of A. yendoi Yamada on the production of pro-inflammatory factors and cytokines stimulated with lipopolysaccharide. In addition, we examined the inhibitory effect of 80% EtOH extract and solvent fractions of A. yendoi Yamada on pro-inflammatory mediators (NO, iNOS, PGE2, and COX-2) in RAW 264.7 cells. In the sequential fractions of n-hexane and EtOAc inhibited the NO and PGE2 production and the protein level of iNOS and COX-2, and protein expression of pro-inflammatory cytokines (TNF-α, and IL-6). These results suggest that A. yendoi Yamada may have significant effects on inflammatory factors and may be provided as possible anti-inflammatory therapeutic seaweed.
Acrosorium yendoi Yamada;Pro-inflammatory factors;Cytokines;Lipopolysaccharide;RAW 264.7 cells;
 Cited by
Burrell, R. 1994. Human responses to bacterial endotoxin. Circ. Shock 43:137-153.

Dinarello, C.A., 2000. Pro-inflammatory cytokines. Chest 118: 503-508. crossref(new window)

Dubois, R., S.B. Abramson, L. Crofford, R.A. Gupta, L.S. Simon., Van De Putte L.B.A and P.E. Lipsky. 1998. Cyclooxygenase in biology and disease. FASEB J. 9:2045-2046.

Gonzalez del Val, A., G. Platas, A. Basilio, A. Cabello, J. Gorrochategui, I. Suay, F. Vincente, E. Portillo, M. Jimenez del Rio, G.G. Reina and F. Pelez. 2001. Screening of antimicrobial activities in red, green and brown macroalgae from Gran Canaria (Canary island, Spain). Int. Microbiol. 4:35-40.

Kaplanski, G., V. Marin, F. Montero-Julian, A. Mantovani and C. Farnarier. 2003. IL-6: a regulator of the transition from neutrophil to monocyte recruitment during inflammation. Trends Immunol. 24:25-29. crossref(new window)

Kim, J.Y., K.S. Jung and H.G. Jeong. 2004. Suppressive effects of the kaweol and cafestol on cuclooxygenase-2 expression in macrophases. FEBS Lett. 569:321-326. crossref(new window)

Kim, R.G., K.M. Shin, S.K. Chun, S.Y. Ji, S.H. Seo, H.J. Park, J.W. Choi and K.T. Lee. 2002. In vitro antiinflammatory activity of the essential oil from Ligularia fischeri var. spiciformis in murine macrophage RAW 264.7 cells. Yakhak Hoeji. 46:343-347.

Ko, Y.J., S.M. Song, W.C. Hyun, S.K. Yang, C.K. Song, D.S. Lee and W.J. Yoon. 2014. Anti-inflammatory effect of Castanopsis cuspidata extracts in Murine Macrophage RAW264.7 cells. Korean J. Plant Res. 27:439-446. crossref(new window)

Lazarov, S., M. Balutsov and E. Ianev. 2000. The role of bacterial endotoxins, receptors and cutokines in the pathogenesis of septic (endotoxin) shock. Vutr. Boles. 32:33-40.

Lee, Y.P. 2008. Marine Algae of Jeju. Academy Book Co., Daegu, Korea. p. 268.

Lee, AK, S.H. Sung, Y.C. Kim and S.G. Kim. 2003. Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF- α and COX-2 expression by sauchinone effects on I-κBa phosphorylation, C/EBP and AP-1 activation. Br. J. Pharmacol. 139:11-22. crossref(new window)

Mu, M.M., D. Chakravortty, T. Sugiyama, N. Koide, K. Takahashi, I. Mori, T. Yoshida and T. Yokochi. 2001. The inhibitory action of quercetin on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophage cells. J. Endotoxin. Res. 7:431-438. crossref(new window)

Namkoong, S., S.A. Jang, E.H. Sohn, J.P. Bak, E. Sohn, H.J. Koo, W.J. Yoon, J.E. Kwon, Y.J. Jeong, X. Meng, H.S. Han and S.C. Kang. 2015. Comparative study of Litsea japonica leaf and fruit extract on the anti-inflammatory effects. Korean J. Plant Res. 28:145-152. crossref(new window)

Park, J.S., E.J. Lee, W.K. Kim and H.S. Kim. 2007. Antiinflammatory effects of short chain fatty acids in IFNgamma-stimulated RAW 264.7 murine macrophage cells; involvement of NF-KappaB and ERK signaling pathways. Int. Immunipharmacol. 7:70-77. crossref(new window)

Piguet, PF, Grau GE, Houser C and Vassalli P. 1991. Tumor necrosis factor is a critical mediators in hapten induced irritant and contact hypersensitivity reaction. J. Exp. Med. 173:673-679. crossref(new window)

Ryu, J.H., H. Ahn, J.Y. Kim and Y.K. Kim. 2003. Inhibitory activity of plant extracts on nitric oxide synthesis in LPSactivated macrophage. Phytother. Res. 17:485-489. crossref(new window)

Smith, W.L., G.R. Michael and D.L. De-Witt. 1996. Prostaglandin endoperoxide H synthases (cyclooxygenases)-1 and -2. J. Biol. Chem. 271:33157-33160. crossref(new window)

Tezuka, Y., S. Irikawa, T. Kaneko, A.H. Banskota, T. Nagaoka, Q. Xiong, K. Hase and S. Kadota. 2001. Screening of Chinese herbal drug extracts for inhibitory activity on nitric oxide production and identification of an active compound of Zanthoxylum bugeanum. J. Ethnopharmacol. 77:209-217. crossref(new window)

Weis, Z.A., L. Cicatiello and H. Esumi. 1996. Regulation of the mouse inducible-type oxide synthase gene pro-moter by interferon-gamma, bacterial lipopo;ysaccharide and NG - monomethyl-L-arginine. Biochem. J. 316:209-215. crossref(new window)

Willeaume, V., V. Kryus, T. Mijatovic and G. Huez. 1996. Tummor necrosis factor-alpha production induced by viruses and by lipopolysaccharides in macrophages: similarities and differences. Journal of Inflammation 46:1-13.

Yamamoto, Y., P. He, T. Klein and H. Friedman. 1994. Endotoxin induced cytotoxicity of macrophages is due to apoptosis caused by nitric oxide production, Journal of Endotoxin Research 1:181-187.

Yoon, W.J., J.A. Lee, K.N. Lim, J.Y. Kim and S.Y. Park. 2007. In vitro anti-inflammatory activity of the Artemisia fukudo extract in murine macrophage RAW 264.7 cells. Korean J. Food SCI. Technol. 39:467-469.