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Ethanol Extract of Hippophae Rhamnoides L. Leaves Inhibits Adipogenesis through AMP-activated protein kinase (AMPK) Activation in 3T3-L1 Preadipocytes
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  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.582-590
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.582
 Title & Authors
Ethanol Extract of Hippophae Rhamnoides L. Leaves Inhibits Adipogenesis through AMP-activated protein kinase (AMPK) Activation in 3T3-L1 Preadipocytes
Jeong, Hyeon Ju; Park, Ju Hee; Kim, Myong-Jo;
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In the present study, we investigated the effect of 70% EtOH extract from Hippophae Rhamnoides L. leaves (HRL) on the anti-obesity effect in 3T3-L1 cells. The effects of HRL on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. In addition, we examined the gene expression levels by using RT-PCR and western blot. The results of this analysis showed that 100 ㎍/㎖ HRL significantly increased the inhibition of lipid accumulation by 82.25%; significantly decreased the mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and fatty acid synthase (FAS) in 3T3-L1 cells as well as the stimulated protein expression of AMP-activated protein kinase (AMPK); and suppressed the expression level of PPARγ. These results suggest that HRL can prevent adipogenesis through activation of AMPKα and inhibition of adipogenesis transcription factors.
Hippophae Rhamnoides L. leaves;HRL;Adipogenesis;3T3-L1;AMPK;
 Cited by
식방풍잎(Peucedanum japonicum Thunberg L.)의 물추출물이 3T3-L1 세포와 고지방식이로 유도된 마우스에서 항비만 효과,정호경;심미옥;장지훈;김태묵;안병관;김민석;정원석;

한국자원식물학회지, 2016. vol.29. 1, pp.1-10 crossref(new window)
고지방 식이 마우스에서 석창포 열수 추출물의 혈중지질 개선 효과,홍선화;김동우;최연식;김다슬;김옥진;

한국자원식물학회지, 2016. vol.29. 4, pp.355-362 crossref(new window)
비파 부위별 추출물이 3T3-L1 세포와 돼지 지방전구세포의 분화에 미치는 효과,이환;이재준;

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Anti-obesity Effects of Peucedanum japonicum Thunberg L. on 3T3-L1 Cells and High-fat Diet-induced Obese Mice, Korean Journal of Plant Resources, 2016, 29, 1, 1  crossref(new windwow)
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