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Ethanol Extract of Hippophae Rhamnoides L. Leaves Inhibits Adipogenesis through AMP-activated protein kinase (AMPK) Activation in 3T3-L1 Preadipocytes
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  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.582-590
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.582
 Title & Authors
Ethanol Extract of Hippophae Rhamnoides L. Leaves Inhibits Adipogenesis through AMP-activated protein kinase (AMPK) Activation in 3T3-L1 Preadipocytes
Jeong, Hyeon Ju; Park, Ju Hee; Kim, Myong-Jo;
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 Abstract
In the present study, we investigated the effect of 70% EtOH extract from Hippophae Rhamnoides L. leaves (HRL) on the anti-obesity effect in 3T3-L1 cells. The effects of HRL on lipid accumulation in 3T3-L1 cells were examined using Oil Red O staining. In addition, we examined the gene expression levels by using RT-PCR and western blot. The results of this analysis showed that 100 ㎍/㎖ HRL significantly increased the inhibition of lipid accumulation by 82.25%; significantly decreased the mRNA expression of sterol regulatory element binding protein-1c (SREBP-1c), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer binding proteins α (C/EBPα), and fatty acid synthase (FAS) in 3T3-L1 cells as well as the stimulated protein expression of AMP-activated protein kinase (AMPK); and suppressed the expression level of PPARγ. These results suggest that HRL can prevent adipogenesis through activation of AMPKα and inhibition of adipogenesis transcription factors.
 Keywords
Hippophae Rhamnoides L. leaves;HRL;Adipogenesis;3T3-L1;AMPK;
 Language
Korean
 Cited by
1.
Effect of Acorus gramineus Water Extract on the Blood Lipid Profiles in High Fat Diet-fed Mice, Korean Journal of Plant Resources, 2016, 29, 4, 355  crossref(new windwow)
2.
Anti-obesity Effects of Peucedanum japonicum Thunberg L. on 3T3-L1 Cells and High-fat Diet-induced Obese Mice, Korean Journal of Plant Resources, 2016, 29, 1, 1  crossref(new windwow)
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