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Triglyceride Control Effect of Agrimonia eupatoria L. in Oleic Acid Induced NAFLD-HepG2 Model
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  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.635-640
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.635
 Title & Authors
Triglyceride Control Effect of Agrimonia eupatoria L. in Oleic Acid Induced NAFLD-HepG2 Model
Sohn, Eun-Hwa; Kim, Taeseong; Jeong, Yong Joon; Han, Hyo-Sang; Lea, Youngsung; Cho, Young Mi; Kang, Se Chan;
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Nonalcoholic fatty liver disease (NAFLD) is a kind of liver inflammation caused by an accumulation of fat in the liver. Patients with NAFLD have an increased risk to develop liver fibrosis, which leads to cirrhosis. To investigate hepatoprotective effects of Agrimonia eupatoria L (A. eupatoria), oleic acid-induced NAFLD in HepG2 cells was used and A. eupatoria was fractionated with ethanol (EtOH), n-hexane, dichloromethane (CH2Cl2), ethyl acetate (EtOAc), n-butanol (BuOH), and H2O. Cells treated with the EtOAc fraction showed the highest lipid accumulation inhibiting effect. A. eupatoria also suppressed triglyceride accumulation and inhibited expression of lipid marker gene, such as a peroxisome proliferator activated receptor γ (PPAR-γ). Moreover, another marker, mRNA expression level of peroxisome proliferator activated receptor α (PPAR-α) was significantly increased by in a dose-dependent manner. These results suggest that A. eupatoria is a potent agent for the treatment of NAFLD.
NAFLD;Agrimonia eupatoria;PPAR-γ;Oleic acid;HepG2;
 Cited by
금앵자 에탄올 추출물에 의한 3T3-L1 지방세포의 분화억제 효과와 그 메커니즘 규명,정현영;정인교;남소연;윤희정;김병우;권현주;

한국미생물생명공학회지, 2016. vol.44. 1, pp.89-97 crossref(new window)
An, R.B., H.C. Kim, G.S. Jeong, S.H. Oh, H. Oh and Y.C. Kim. 2005. Constituents of the aerial parts of Agrimonia pilosa. J Nat Prod. 11:196-199.

Choi, M.Y., H.J. Woo, Y.C. Kim and J.H. Lee. 2009. Effects of gamisaenggan-tang on high fat diet-induced nonalcoholic fatty liver disease. Korean J. Orient Int. Med. 30(2):365-374.

Comelius, P., O.A. MacDougald and M.D. Lane. 1994. Regulation of adipocyte development. Annu Rev Nutr. 14:99-129. crossref(new window)

Correia, H.S., M.T. Batista and T.C. Dinis. 2007. The activity of an extract and fraction of Agrimonia eupatoria L. against reactive species. Biofactors 29:91-104. crossref(new window)

Cwikla, C., K. Schmidt, A. Matthias, K.M. Bone, R. Lehmann and E. Tiralongo. 2010. Investigations into the antibacterial activities of phytotherapeutics against Helicobacter pylori and Campylobacter jejuni. Phytother Res. 24:649-656.

Gray, A. M. and P. R. Flatt. 1998. Actions of the traditional anti-diabetic plant, Agrimony eupatoria (agrimony): effects on hyperglycaemia, cellular glucose metabolism and insulin secretion. Br. J. Nutr. 80(1):109-114. crossref(new window)

Hashimoto, T., W.S. Cook, C. Qi, A.V. Yeldandi, J.K. Reddy and M.S. Rao. 2000. Defect in peroxisome proliferator-activated receptor alpha-inducible fatty acid oxidation determines the severity of hepatic steatosis in response to fasting. J. Biol. Chem. 275:28918-28928. crossref(new window)

Ivanova, D., D. Vankova and M. Nashar. 2013. Agrimonia eupatoria tea consumption in relation to markers of inflammation, oxidative status and lipid metabolism in healthy subjects. Arch. Physiol. Biochem. 119:32-37. crossref(new window)

Jeon, T., S.G. Hwang, S. Hirai, T. Matsui, H. Yano, T. Kawda, B.U. Lim and D. Ki. 2004. Red yeast rice extracts suppress adipogenesis by down-regulating adipogenic transcription factors and gene expression in 3T3-L1 cells. Life Sci. 75:3195-3203. crossref(new window)

Kato, H., W. Li, M. Koike, Y. Wang and K. Koike. 2001. Phenolic glycosides from Agrimonia pilosa. Phytochemistry 71:1925-1929.

Kim, S.M., D.K. Yoon, B.S. Yang and K.H. Cho. 2005. An activation of peroxisome proliferator-activated receptor δ attenuate alcoholic liver disease and nonalcoholic fatty liver disease in rats. J. Korean Soc. Food Sci. Nutr. 14(1):29-38.

Kim, S.M., J.A. Kim, J.H. Han, K.H. Cho and D.K. Yoon. 2006. Nonalcoholic fatty liver disease and metabolic syndrome in nonobese, Nondiabetic adults. J. Korean Soc. Study Obes. 15:(1) 44-51.

Kliewer, S.A. and T.M. Wilson. 1998. The nuclear receptor PPAR-γ: bigger than fat. Curr. Opin. Genet. Dev. 8:576-581. crossref(new window)

Kwon, D.H., H.Y. Kwon, H.J. Kim, E.J. Chang, M.B. Kim, S.K. Yoon, E.Y. Song, D.Y. Yoon, Y.H. Lee, I.S. Choi and Y.K. Choi. 2005. Inhibition of hepatitis B virus by an aqueous extract of Agrimonia eupatoria L. Phytother Res. 19:355-358. crossref(new window)

Lapsys, N.M., A.D. Kriketos, M. Lim-Frase, A.M. Poynten, A. Lowy, S.M. Furler, D.J. Chisholm and G.J. Cooney. 2000. Expression of genes involved in lipid metabolism correlate with peroxisome proliferator-activated receptor gamma expression in human skeletal muscle. J. Clin. Endocrinol. Metab. 85:4293-4297.

Lee, J.A., E.K. Ahn, S.S. Hong and J.S. Oh. 2012. Anti-obesity effect of ethyl acetate extracts from Agrimonia pilosa Ledeb. in 3T3-L1 preadipocytes. J. Korean Soc. Food Sci. Nutr. 41(2):161-167. crossref(new window)

Ludwig, J., T.R. Viggiano, D.B. Mcgill and B.J. Oh. 1980. Nonalcoholic steatohepatitis: Mayo clinic experiences with a hitherto unnamed disease. Mayo Clin. Proc. 55:434-438

Park, M.Y., H.H. Jang, J.Y. Lee, Y.M. Lee, J.H. Kim, J.H. Park and D.S. Park. 2012. Effect of Hog Millet supplementation on hepatic steatosis and insulin resistance in mice fed high-fat diet. J. Korean Soc. Food Sci. Nutr. 41(4):501-509. crossref(new window)

Rosen, E.D., C.J. Walkey, P. Puigserver and B.M. Spiegelman. 2000. Transcriptional regulation of adipogenesis. Genes & Dev. 14:1293-1307.

Smith, B.W. and L.A. Adams. 2011. Non-alcoholic fatty liver disease. Crit Rev Clin Lab Sci. 48:97-113. crossref(new window)

Sung, T.S. and G.M. Son. 1994. Effect of Cnidi rhizome boiling extract solution on enzyme and hormone of plasma, and liver in the fatted rats induced by high fat dietary. Korean J. Food & Nutrition 7(2):108-113.

Wang, S., H.N. Song, W.I. Choi, J.H. Park, Y.J. Jeong, S.C. Kang, S.K. Ko and D.H. Bhang. 2012. Identification of anti-obesity constituents from Yukeuigambitang. Korean J. Plant Res. 25(2):271-276. crossref(new window)

Ye, J.M., P.J. Doyle, M.A. Iglesias, D.G. Watson, G.J. Cooney and E.W. Kraegen. 2001. Peroxisome proliferator activated receptor (PPAR)-alpha activation lowers muscle lipids and improves insulin sensitivity in high fat-fed rats: comparison with PPAR-gamma activation. Diabetes 50:411-417. crossref(new window)

Yoo, J. and J. Lee. 2010. Effects of Saenggantanggami-bang on nonalcoholic fatty liver disease model induced by fatty acids. Korean J. Orient Int. Med. 31(2):143-157.

Yun, K.S., H.J. Woo, J.H. Lee and Y.C. Kim. 2009. Effect of Injinchunggan-tang & Injinsaryung-san on NASH induced by MCD-diet in A/J mice. Korean J. Orient Int. Med. 30(2):410-421.

Zhu, L., J. Tan, B. Wang, R. He, Y. Liu and C. Zheng. 2009. Antioxidant activities of aqueous extract from Agrimonia pilosa Ledeb and its fractions. Chem. Biodivers. 6:1716-1726. crossref(new window)