Advanced SearchSearch Tips
Development of a CAPS Marker Derived from the Pg-Actin Gene Sequences and RAPD Markers in Platycodon grandiflorum
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.648-655
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.648
 Title & Authors
Development of a CAPS Marker Derived from the Pg-Actin Gene Sequences and RAPD Markers in Platycodon grandiflorum
Kim, Munhwi; Jeong, Eunah; Jeong, Jeongsu; Kwon, Soontae; Jeon, Ikjo; Jeong, Jeong Hag; Lee, Je Min; Yeam, Inhwa;
  PDF(new window)
Balloon flower (Platycodon grandiflorum A. DC.) is a perennial plant of mainly Campanulaceae family, which have been widely used as a food ingredient and herbal medicine in East Asia. Although demands on related products and yearly cultivation area for balloon flower are increasing, diverse fundamental technologies and molecular breeding studies are not very well supported in Platycodons. In this study, 30 random amplification of polymorphic DNA (RAPD) primers were test in an attempt to explore genetic diversities. In addition, sequences information of the actin gene, a well conserved gene encoding a globular protein that forms microfilaments, was retrieved and analyzed. Two actin homologs were recovered; 3.4 kb fragment is a Pg-actin and 1.4 kb fragment is a Pg-actin homolog with 28.6% similarity. We have confirmed that the Pg-actin gene is configured into 4 exons and 3 introns. A single nucleotide polymorphism (SNP), G↔A, was detected on the intron 3, which served as a target for the CAPS marker development. The marker Pg-Actin-Int3 was applied to 32 balloon flower accessions. Balloon flower DNA sequence information generated in this study is expected to contribute to the analysis and molecular breeding and genetic diversity analysis of balloon flowers.
Actin gene;Balloon flower;CAPS marker;Platycodon grandiflorum;
 Cited by
Aggarwal, R.K., P.S. Hendre, R.K. Varshney, P.R. Bhat, V. Krishnakumar and L. Singh. 2007. Identification, characterization and utilization of EST-derived genic microsatellite markers for genome analyses of coffee and related species. Theor. Appl. Genet. 114(2):359-372. crossref(new window)

Bang, K.H., C.G. Park, D.C. Jin, H.S. Kim, H.W. Park, C.H. Park and N.S. Seong. 2003. Discrimination of species specific DNA markers using RAPD and AFLP analysis between Atractylodes japonica Koidz. and Atractylodes macrocephala Koidz. Korean J. Medicinal Crop Sci. 11:268-273.

Drouin, G. and G.A. Dover. 1990. Independent gene evolution in the potato actin gene family demonstrated by phylogenetic procedures for resolving gene conversions and the phylogeny of angiosperm actin genes. J. Mol. Evol. 31(2):132-150. crossref(new window)

Fedorov, A.A. 1957. Campanulaceae. In flora of the U.S.S.R., Vol. 24. Shishkin, B. K. (eds.), Akademia Nauk, Moskva Press, Russia. pp. 459-475.

Harn, C. and Y. Lee. 1976. Induction of plantlets in the anther culture of Platycodon grandiflorum. Korean J. Plant Tissue Cult. 4(1):1-3 (in Korean).

Hong, J.B. 1990. Plant actin gene. Mol. Biol. News 2(1):41-50 (in Korean).

Huang, S., B. Zhang, D. Milbourne, L. Cardle, G. Yang and J. Guo. 2000. Development of pepper SSR markers from sequence databases. Euphytica 117:163-167.

Jeong, C.H. and K.H. Shim. 2006. Chemical composition and antioxidative activities of Platycodon grandiflorum leaves and stems. Kor. J. Soc. Food Sci. Nutr. 35(5):511-515 (in Korean). crossref(new window)

Kang, B.C., S.H. Nahm, J.H. Huh, H.S. Yoo, J.W. Yu, M.H. Lee and M.H. Kim. 2001. An interspecific (Capsicum annuum × C. chinese) F2 linkage map in pepper using RFLP and AFLP markers. Theor. Appl. Genet. 102:531-539. crossref(new window)

Kim, S.C., Y.H. Jung, K.C. Seong, S.J. Chun, C.H. Kim, C.K. Lim, J.H. Joa and D.S. Lee. 2014. Development of a SCAR marker for sex identification in asparagus. Korean J. Plant Res. 27(3):236-241. crossref(new window)

Kim, T.W., S.J. Lee, M.B. Kim, C.G. Park, Y.W. Shin, Y.S. Cho and S.W. Lee. 2014. Differentiation of indigenous balloon flower (Platycodon grandiflorum DC.) germ lines in South Korea by using RAPD analyses. J. Plant Biotechnol. 41:19-25 (in Korean). crossref(new window)

Konieczny, A. and F.M. Ausubel. 1993. A procedure for mapping arabidopsis mutations using co-dominant ecotype-specific PCR-based markers. Plant J. 4(2):403-410. crossref(new window)

Lee, C.B. 1980. Illustrated flora of Korea, Haengmun Press, Seoul, Korea. p. 725.

Lee, J.M., S.H. Nahm, Y.M. Kim and B.D. Kim. 2004. Characterization and molecular genetic mapping of microsatellite loci in pepper. Theor. Appl. Genet. 108:619-627. crossref(new window)

Lee, S.I. 1981. Chinese parmaceutics. Sooseowon Press, Seoul, Korea. p. 129.

Lim, K.H. 1971. A Medicinal phytology (The details). Dong Myoung Press, Seoul, Korea. p. 281.

Lin, K.H., H.F. Lo, S.P. Lee, C.G. Kuo, J.T. Chen and W.L., Yeh. 2006. RAPD markers for the identification of yield traits in tomatoes under heat stress via bulked segregant analysis. Hereditas 143:142-154. crossref(new window)

Long, W., Y. Li, W. Zhou, H.Q. Ling and S. Zheng. 2013. Sequencebased SSR marker development and their application in defining the introgressions of LA0716 (Solanum pennellii) in the background of cv. M82 (Solanum lycopersicum). PLoS One 8(12):e81091. crossref(new window)

Mabberley, D.J. 1987. The plant book. Cambridge University Press, Cambridge, United Kingdom. p. 461.

Nairn, C.J., L. Winesett and R.J. Ferl. 1988. Nucleotide sequence of an actin gene from Arabidopsis thaliana. Gene 65:247-257. crossref(new window)

Paran, I. and R.W. Michelmore. 1993. Development of reliable PCR based markers linked to downy mildew resistance genes in lettuce. Theor. Appl. Genet. 85:985-993.

Park, C.G. 2006. Classification of Platycodon grandiflorum(JACQ.) A. DC. collections by growth characteristics and RAPD analysis. Department of Agronomy Grauate School, Ph.D. Thesis, Kyungpook National Univ., Korea.

Park, C.G., K.H. Bang, O.T. Kim, D.C. Jin, D.H. Kim, J.S. Sung, N.S. Seong, H.W. Park. and S.C. Lee. 2007. Development of SCAR marker for discriminating between violet flowered lines and white flowered lines in chinese bellflower (Platycodon grandiflorum A.). Kor. J. Medicinal Crop Sci. 15(1):1-5.

Park, S.J., J.W. Kim, S.J. Park and T.J. Kim. 2012. Effects of Platycodon grandiflorum including platycodin DinIgE/Ag-Induced type I hypersensitivity. Life Sci. 22:595-599 (in Korean). crossref(new window)

Peremyslov, V.V., T.C. Mockler, S.A. Filichkin, S.E. Fox, P. Jaiswal, K.S. Makarova, E.V. Koonin and V.V. Dolja. 2011. Expression, splicing, and evolution of the myosin gene family in plants. Plant Physiol. 155(3):1191-1204. crossref(new window)

Shin, S.S., J.S. Choi and M.K. Huh. 2009. ISSR markers of authentication for korean and chinese Platycodon grandiflorum. Kor. J. Oriental Physiol. Pathol. 23(1):214-218.

Shirasawa, K., S. Isobe, S. Tabata and H. Hirakawa. 2014. Kazusa Marker DataBase: A database for genomics, genetics, and molecular breeding in plants. Breed. Sci. 64(3):264-271. crossref(new window)

Son, I.H., Y.H. Park, S.I. Lee, H.D. Yang and H.I. Moon. 2007. Neuroprotective activity of triterpenoid saponins from Platycodi radix against glutamate-induced toxicity in primary cultured rat cortical cells. Molecules 12(5):1147-1152. crossref(new window)

Wen, S.S., D.W. He, C.M. Liao, J. Li, G.Q. Wen and X.H. Liu. 2014. Cloning and sequence analysis of an actin gene in aloe. Genet Mol. Res. 13(3):4949-4955. crossref(new window)

Yi, G., J.M. Lee, S. Lee, D. Choi and B.D. Kim. 2006. Exploitation of pepper EST-SSRs and an SSR-based linkage map. Theor. Appl. Genet. 114(1):113-130. crossref(new window)

Zhang, D., Q. Du, B. Xu, Z. Zhang and B. Li. 2010. The actin multigene family in Populus: organization, expression and phylogenetic analysis. Mol. Genet. Genomics 284(2):105-119. crossref(new window)

Korean Statistical Information Service, 2010-2012 Search board: Wild green plants of cities and countries index. Retrieved Oct. 15. 2014. from the World Wide Web: