Advanced SearchSearch Tips
Development of Tetraploid Watermelon Using Chromosome Doubling Reagent Treatments
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.656-664
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.656
 Title & Authors
Development of Tetraploid Watermelon Using Chromosome Doubling Reagent Treatments
Oh, Sang A; Min, Kwang Hyun; Choi, Yong Soo; Park, Sang Bin; Kim, Young Cheol; Cho, Song Mi;
  PDF(new window)
To produce high quality watermelon, three tetraploid watermelon breeding lines (‘SA03-1’, ‘SA06-1’ and ‘SB01-1’) were developed by treatment with different chromosome doubling reagents. To identify the optimal tetraploid inductive conditions, the three watermelon breeding lines were selected by counting the number of doubled chloroplasts in guard cells. Tetraploid induction rates differed depending on the genotypes and treatment with doubling reagents. However, the highest induction rate occurred with 1.0% colchicine (82.2%). These putative tetraploid lines were re-confirmed for ploidy using flow cytometric analysis and chromosome counting. The internode length of the tetraploid breeding lines was different when the leaf size was larger in all three tetraploid lines compared to their diploids. The fruit weight of the tetraploid fruits for ‘SA03-1’ and ‘SB01-1’ was lower than for their diploid, and the rind thickness and total sugar content (°Brix) of tetraploid SB01-1 were significantly different from those of its diploid. Tetraploid lines were sterile, yielded a lower number of seeds per fruit for ‘SA03-1’ (21), ‘SA06-1’ (62), and ‘SB01-1’ (34.7), and the seeds were larger and thicker than those of their diploids. These tetraploid breeding results will be useful for breeding new seedless watermelon cultivars.
Citrullus lanatus;Colchicine;2,6-dinitroaniline;Polyploidy;Seedless;
 Cited by
Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata,;;;;;;;;

한국자원식물학회지, 2016. vol.29. 3, pp.347-354 crossref(new window)
Comparison of Yield and Growth Characteristics of Platycodon grandiflorum According to the Ploidy Levels and Growing Conditions,;;;;;;;;

한국자원식물학회지, 2016. vol.29. 3, pp.331-338 crossref(new window)
Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata, Korean Journal of Plant Resources, 2016, 29, 3, 347  crossref(new windwow)
Comparison of Yield and Growth Characteristics of Platycodon grandiflorum According to the Ploidy Levels and Growing Conditions, Korean Journal of Plant Resources, 2016, 29, 3, 331  crossref(new windwow)
Anonymous (2012). "Annula world statistics", (weblink:

Berdahl, J.D and R.E. Ries. 1997. Development and vigor of diploid and tetraploid Russian wild rye seedling. J. Range Management 50:80-84. crossref(new window)

Cho, S.M., S.A. Oh, Y.S. Choi and S.B. Park. 2014. Effect of plant growth regulators on regeneration from the cotyledon explants in watermelon (Citrullus lanatus (thumb.) Matsum. & Nakai). Korean J. Plant Res. 27(1):51-59 (in Korean). crossref(new window)

Compton, M.E. and D.J. Gray. 1994. Adventitious shoot organogenesis and plant regeneration from cotyledon of tetraploid watermelon. HortScience 29:211-213.

Compton, M.E., D.J. Gray and G.W. Elmstrom. 1996. Identification of tetraploid regenerants from cotyledons of diploid watermelon cultured in vitro. Euphytica 87:165-172. crossref(new window)

Compton, M.E., N. Barnett and D.J. Gray. 1999. Use of fluorescein diacetate (FDA) to determine ploidy of in vitro watermelon shoots. Plant Cell Tiss. Org. Cult. 58:199-203. crossref(new window)

Compton, M.E., D.J. Gray and V.P. Gaba. 2004. Use of tissue culture and biotechnology for the genetic improvement of watermelon. Plant Cell Tiss. Org. Cult. 77:231-243. crossref(new window)

Galbraith, D.W., K.R. Harkins, J.R. Maddox, N.M. Ayres, D.P. Sharma and E. Firoozabady. 1983. Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220:1049-1051. crossref(new window)

Hansen, N.J.P. and S.B. Andersen. 1996. In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin, and APM in Brassica napus microspore culture. Euphytica 88:159-164. crossref(new window)

Haskell, G. and E. B. Paterson. 1962. The Phase Contrast Microscope in Plant Cytology. Genetica 33:52-58.

Hayata, Y., Y. Niimi and N. Iwasaki. 1995. Synthetic Cytokinin- 1-(2-chloro-4-pyridyl)-3-phenylurea (CPPU)-promotes fruit set and induces parthenocarpy in watermelon. J. Am. Soc. Hort. Sci. 120:997-1000.

Henderson, W.R. 1977. Effect of cultivar, polyploidy and reciprocal hybridization on characters important in breeding triploid seedless watermelon hybrids. Proc. Am. Soc. Hort. Sci. 102:293-297.

Jaskani, M.J., I.A. Khan and S. Husnain. 1996. Morphological description of citrus colchiploid. Proc. Intl. Soc. Citriculture 1:130-132.

Jaskani, M.J., S.W. Kwon, G.C. Koh, Y.C. Huh and B.R. Ko. 2004. Induction and characterization of tetraploid watermelon. J. Korean Soc. Hort. Sci. 45:60-65.

Jaskani, M.J., S.W. Khan and D.H. Kim. 2005. Flow cytometry of DNA contents of colchicines treated watermelon as a ploidy screening method at M1 stage. Pak.J. Bot. 37:685-696.

Kihara, H. 1951. Triploid watermelons. Proc. Amer. Soc. Hort. Sci. 58:217-230.

Koh, G.C. 2002. Tetraploid production of Moodeungsan watermelon. J. Korean Soc. Hort. Sci. 43: 671-676 (in Korean).

Lower, R.L. and K.W. Johnson. 1969. Observations on sterility of induced autotetraploid watermelons. J. Amer. Soc. Hort. Sci. 94:367-369.

Marr, C.W. and K.L.B. Gast. 1991. Reaction by consumers in a “famers” market to price for seedless watermelon and ratings of eating quality. Hort. Technol. 1:105-106.

NeSmith, D.S. and J.R. Duval. 2001. Fruit set of triploid watermelons as a function of distance from a diploid pollinizer. HortSceince 36(1):60-61.

Sari, N., K. Abak and M. Pitrat. 1999. Comparison of ploidy level screening methods in watermelon: Citrullus lanatus (Thunb.) Matsum. And Nakai. Sci. Hortic. 82:265-277. crossref(new window)

Sugiyama, K. and M. Morishita 2000. Production of seedless watermelon using soft-X-irradated pollen. Scientia Hort. 84:255-264. crossref(new window)

Suying, T., H. Xiuqiang, L. Jiwei and L. Wenge. 1995. Raising the frequency of inducing tetraploid watermelon by treating colchicine. Acta Hort. 402:18-22.

van Duren, M., R. Morpurg, J. Dolezel and R. Afza. 1996. Induction and verification of autotetraploids in diploid banana (Musa acuminata) by in vitro techniques. Euphytica 88:25-34. crossref(new window)

Velleux, R.E. and A.A.T. Johnson. 1998. Somaclonal variation: molecular analysis, transformation interaction, and utilization: In Plant Breeding Reviews, Janick, J. (Ed.), John Wiley & Sons, Inc., New York (USA). pp. 229-267.

Wall, J.R. 1960. Use of marker genes in producing triploid watermelon. Proc. Am. Soc. Hort. Sci. 76:577-581.

Ye, Y.M., J. Tong, X.P. Shi, W. Yuan and G.R. Li. 2010. Morphological and cytological studies of diploid and colchicine-induced tetraploid lines of carpe myrtle (Lagerstromemia indica L.). Sci. Hortic. 124:95-101. crossref(new window)