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Development of Tetraploid Watermelon Using Chromosome Doubling Reagent Treatments
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  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 5,  2015, pp.656-664
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.5.656
 Title & Authors
Development of Tetraploid Watermelon Using Chromosome Doubling Reagent Treatments
Oh, Sang A; Min, Kwang Hyun; Choi, Yong Soo; Park, Sang Bin; Kim, Young Cheol; Cho, Song Mi;
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 Abstract
To produce high quality watermelon, three tetraploid watermelon breeding lines (‘SA03-1’, ‘SA06-1’ and ‘SB01-1’) were developed by treatment with different chromosome doubling reagents. To identify the optimal tetraploid inductive conditions, the three watermelon breeding lines were selected by counting the number of doubled chloroplasts in guard cells. Tetraploid induction rates differed depending on the genotypes and treatment with doubling reagents. However, the highest induction rate occurred with 1.0% colchicine (82.2%). These putative tetraploid lines were re-confirmed for ploidy using flow cytometric analysis and chromosome counting. The internode length of the tetraploid breeding lines was different when the leaf size was larger in all three tetraploid lines compared to their diploids. The fruit weight of the tetraploid fruits for ‘SA03-1’ and ‘SB01-1’ was lower than for their diploid, and the rind thickness and total sugar content (°Brix) of tetraploid SB01-1 were significantly different from those of its diploid. Tetraploid lines were sterile, yielded a lower number of seeds per fruit for ‘SA03-1’ (21), ‘SA06-1’ (62), and ‘SB01-1’ (34.7), and the seeds were larger and thicker than those of their diploids. These tetraploid breeding results will be useful for breeding new seedless watermelon cultivars.
 Keywords
Citrullus lanatus;Colchicine;2,6-dinitroaniline;Polyploidy;Seedless;
 Language
Korean
 Cited by
1.
Comparison of Yield and Growth Characteristics of Platycodon grandiflorum According to the Ploidy Levels and Growing Conditions, Korean Journal of Plant Resources, 2016, 29, 3, 331  crossref(new windwow)
2.
Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata, Korean Journal of Plant Resources, 2016, 29, 3, 347  crossref(new windwow)
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