Advanced SearchSearch Tips
Protective Effects of Ramie (Boehmeria nivea) against Oxidative Stress in C6 Glial Cells
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 6,  2015, pp.675-681
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.6.675
 Title & Authors
Protective Effects of Ramie (Boehmeria nivea) against Oxidative Stress in C6 Glial Cells
Wang, Xiaoning; Cho, Sunghun; Kim, Ho Bang; Jung, Yong-Su; Cho, Eun Ju; Lee, Sanghyun;
  PDF(new window)
β amyloid protein (Aβ) plays a critical role in the pathogenesis of Alzheimer`s disease (AD) and possibly in Aβ-induced mitochondrial dysfunction and oxidative stress. Aβ can directly cause reactive oxygen species (ROS) production. Overproduction of ROS is considered to be involved in the pathogenesis of neurodegeneration of AD. Here, we investigated 9 kinds of ramie (Boehmeria nivea, (L.) Gaud., BN; hereafter denoted as BN) for their protective action against oxidative stress in a cellular system using C6 glial cells. We observed loss of cell viability and high levels of ROS generation after treatment with hydrogen peroxide (H2O2) and Aβ25-35. However, treatments with BN extracts led to an increase in cell viability and decrease in ROS production induced by H2O2 and Aβ25-35. In particular, the extracts of BN-01 (seobang variety from Seocheon) and BN-09 (local variety from Yeonggwang) showed excellent anti-oxidative properties. This indicates that BN extracts could prevent neurodegeneration by reducing oxidative stress in cells.
Boehmeria nivea;Alzheimer’s disease;Reactive oxygen species;C6 glial cell;
 Cited by
Angelini, L.G., A. Lazzeri, G. Levita, D. Fontanelli and C. Bozzi. 2000. Ramie (Boehmeria nivea (L.) Gaud.) and Spanish broom (Spartium junceum L.) fibres for composite materials: agronomical aspects, morphology and mechanical properties. Ind. Crops Prod. 11:145-161. crossref(new window)

Araujo, D.M. and C.W. Cotman. 1992. Beta-amyloid stimulates glial cells in vitro to produce growth factors that accumulate in senile plaques in Alzheimer's disease. Brain Res. 569: 141-145. crossref(new window)

Behl, C., J.B. Davis, R. Lesley and D. Schubert. 1994. Hydrogen peroxide mediates amyloid beta protein toxicity. Cell 77: 817-827. crossref(new window)

Butterfield, D.A. and C.M. Lauderback. 2002. Lipid peroxidation and protein oxidation in Alzheimer’s disease brain: potential causes and consequences involving amyloid beta-peptide-associated free radical oxidative stress. Free Radic. Biol. Med. 32:1050-1060. crossref(new window)

Butterfield, D.A., J. Drake, C. Pocernich and A. Castegna. 2001. Evidence of oxidative damage in Alzheimer’s disease brian: central role for amyliod beta-peptide. Trends Mol. Med. 7:548-554. crossref(new window)

Byun, Y.J., S.K. Kim, Y.M. Kim, G.T. Chea, S.W. Jeong and S.B. Lee. 2009. Hydrogen peroxide induces autophagic cell death in C6 glioma cells via BNIP3-mediated suppression of the mTOR pathway. Neurosci. Lett. 461:131-135. crossref(new window)

Castoria, R., L. Caputo, C.F. De and C.V. De. 2003. Resistance of postharvest biocontrol yeasts to oxidative stress: a possible new mechanism of action. Phytopathology 93:564-572. crossref(new window)

Chen, Y. and S.B. Gibson. 2008. Is mitochondrial generation of reactive oxygen species a trigger for autophagy? Autophagy 4:246-248. crossref(new window)

Di Carlo, M., D. Giacomazza, P. Picone, D. Nuzzo and P.L. San Biaqio. 2012. Are oxidative stress and mitochondrial dysfunction the key players in the neurodegenerative diseases? Free Radic. Res. 46:1327-1338. crossref(new window)

Fukumoto, L.R. and G. Mazza. 2000. Assessing antioxidant and prooxidant activities and phenolic compounds. J. Agric. Food Chem. 48:3597-3604. crossref(new window)

Gill, S.S. and N. Tuteja. 2010. Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol. Biochem. 48:909-930. crossref(new window)

Gupta, K. and D.S. Wagle. 1988. Nutritional and antinutritional factors of green leafy vegetables. J. Agric. Food Chem. 36:472-474. crossref(new window)

Han, W. and M.H. Wang. 2010. Phenylalanine ammonia – Lyase gene (NtPAL4) induced by abiotic stresses in tobacco (Nicotiana tabacum). Korean J. Plant Res. 23:535-540.

Huang, K.L., Y.K. Lai, C.C. Lin and J.M. Chang. 2006. Inhibition of hepatitis B virus production by Boehmeria nivea root extract in HepG2 2.2.15 cells. World J. Gastroenterol. 12:5721-5725. crossref(new window)

Juvenet, J. 1889. Ramie. J. Franklin Inst. 128:371-376. crossref(new window)

Kim, S.H., M.J. Sung, J.H. Park, H.J. Yang and J.T. Hwang. 2013. Boehmeria nivea stimulates glucose uptake by activating peroxisome proliferator-activated receptor gamma in C2C12 cells and improves glucose intolerance in mice fed a high-fat diet. Evid. Based Complement. Alternat. Med. 2013:1-9.

Lee, A.Y., X. Wang, D.G. Lee, Y.M. Kim, Y.S. Jung, H.B. Kim, H.Y. Kim, E.J. Cho and S. Lee. 2014. Various biological activities of ramie (Boehmeria nivea). J. Appl. Biol. Chem. 57:279-286. crossref(new window)

Lee, E. and E.J. Cho. 2007. Effects of Coptidis Rhizoma on lowering lipid and oxidative stress. Korean J. Plant Res. 20:544-547.

Liu, F., X. Liang, N. Zhang, Y. Huang and S. Zhang. 2001. Effect of growth regulators on yield and fiber quality in ramie (Boehmeria nivea (L.) Gaud.), China grass. Field Crops Res. 69:41-46. crossref(new window)

Macarisin, D., S. Droby, G. Bauchan and M. Wisniewski. 2010. Superoxide anion and hydrogen peroxide in the yeast antagonist-fruit interaction: a new role for reactive oxygen species in postharvest biocontrol? Postharvest Biol. Technol. 58:194-202. crossref(new window)

Madeo, F., E. FÖhlich, M. Ligr, M. Grey, S.J. Sigrist, D.H. Wolf and K.U. FrÖhlich. 1999. Oxygen stress: a regulator of apoptosis in yeast. J. Cell Biol. 145:757-767. crossref(new window)

Markesbery, W.R. 1997. Oxidative stress hypothesis in Alzheimer’s disease. Free Radic. Biol. Med. 23:134-147. crossref(new window)

Miller, E.W., B.C. Dickinson and C.J. Chang. 2010. Aquaporin-3 mediates hydrogen peroxide uptake to regulate downstream intracellular signaling. Proc. Natl. Acad. Sci. USA. 107: 15681-15686. crossref(new window)

Mosmann, T. 1983. Rapid colormetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Methods 65:55-63. crossref(new window)

Myhre, O. 2003. Evaluation of the probes 2',7'-dichlorofluorescin diacetate, luminal, and lucigenin as indicators of reactive species formation. Biochem. Pharmacol. 65:1575-1582. crossref(new window)

Nho, J.W., I.G. Hwang, H.Y. Kim, Y.R. Lee, K.S. Woo, B.Y. Hwang, S.J. Chang, J.S. Lee and H.S. Jeong. 2010. Free radical scavenging, angiotensin I-converting enzyme (ACE) inhibitory, and in vitro anticancer activities of ramie (Boehmeria nivea) leaves extracts. Food Sci. Biotechnol. 19: 383-390. crossref(new window)

Nunomura, A., G. Perry, G. Aliev, K. Hirai, A. Takeda, E.K. Balraj, P.K. Jones, H. Ghanbari, T. Wataya, S. Shimohama, S. Chiba, C.S. Atwood, R.B. Petersen and M.A. Smith. 2001. Oxidative damage is the earliest event in Alzheimer disease. J. Neuropathol. Exp. Neurol. 60:759-767. crossref(new window)

Park, J.E., H.J. Bae, N.M. Joo, S.J. Lee, H.A. Jung and E.M. Ahn. 2010. The quality characteristics of cookies with added Boehmeria nivea. Korean J. Food Nutr. 23:446-452 (in Korean).

Pike, C.J., A.J. Walencewicz-Wasserman, J. Kosmoski, D.H. Cribbs, C.G. Glabe and C.W. Cotman. 1995. Structure-activity analyses of β-amyloid peptidesl; contribution of the β25-35 region to aggregation and neurotoxicity. J. Neurochem. 64:253-265.

Santos, M.J., R.A. Quintanilla, A. Toro, R. Grandy, M.C. Dinamarca, J.A. Godoy and N.C. Inestrosa. 2005. Peroxisomal proliferation protects from beta-amyloid neurode-generation. J. Biol. Chem. 280:41057-41068. crossref(new window)

Shao, L.J. and J.N. Wang. 2010. Studies on the chemical constituents of radix Boehmeriae. J. Chin. Med. Mat. 33: 1091-1093.

Sung, M.J., M. Davaatseren, S.H. Kim, M.J. Kim and J.T. Hwang. 2013. Boehmeria nivea attenuates LPS-induced inflammatory markers by inhibiting p38 and JNK phosphorylations in RAW264.7 macrophages. Pharm. Biol. 51:1131-1136. crossref(new window)

Tatone, C., G.D. Emidio, M. Ventol, R. Ciriminna and P.G. Artini. 2010. Cryopreservation and oxidative stress in reproductive cells. Gynecol. Endocrinol. 26:563-567. crossref(new window)

Tian, X.Y., M. Xu, B. Deng, K.S. Leung, K.F. Cheng, Z.Z. Zhao, S.P. Zhang, Z.J. Yang, P.X. Deng, D.Y. Xu, X.P. Xu, I. Koo and M. Wong. 2011. The effects of Boehmeria nivea (L.) Gaud. on embryonic development: in vivo and in vitro studies. J. Ethnopharmacol. 134:393-398. crossref(new window)

Wang, J.Y., L.L. Wen, Y.N. Huang, Y.T. Chen and M.C. Ku. 2006. Dual effects of antioxidants in neurodegeneration: direct neuroprotection against oxidative stress and indirect protection via suppression of glia-mediated inflammation. Curr. Pharm. Des. 12:3521-3533. crossref(new window)

Woo, H.A., H.Z. Chae, S.C. Hwang, K.S. Yang, S.W. Kang, K. Kim and S.G. Rhee. 2003. Reversing the inactivation of peroxiredoxins caused by cysteine sulfinic acid formation. Science 300:653-656. crossref(new window)

Wood, I.M. and J.F. Angus. 1974. A review of prospective crops for the Ord irrigation area. II. Fibre Crops. CSIRO Aust. Div. Land Use Res. Tech. Pap. 36:1-27.

Xio, X.Q., R. Wang, Y.F. Han and X.C. Tang. 2000. Protective effects of huperzine A on $\beta$-amyloid25-35 induced oxidative injury in rat pheochromocytoma cells. Neurosci. Lett. 286:155-158. crossref(new window)

Xu, Q.M., Y.L. Liu, X.R. Li, X. Li and S.L. Yang. 2011. Three new fatty acids from the roots of Boehmeria nivea (L.) Gaudich and their antifungal activities. Nat. Prod. Res. 25:640-647. crossref(new window)

Yatin, S.M., S. Varadarajan, C.D. Link and D.A. Butterfield. 1999. In vitro and in vivo oxidative stress associated with Alzheimer's amyloid beta-peptide (1-42). Neurobiol. Aging 20:325-330. crossref(new window)

Yoon, S.J. and M.S. Jang. 2006. Characteristics of quality in Jeolpyun with different amounts of ramie. Korean J. Food Cookery Sci. 22:636-641 (in Korean).

Zhao, X., L. Yuan, H. Yu, Y. Xi, W. Ma, X. Zhou, J. Ding and R. Xiao. 2013. Genistein inhibited amyloid-β induced inflammatory damage in C6 glial cells. Arch. Med. Res. 45:152-157.