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Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites
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  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 6,  2015, pp.743-751
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.6.743
 Title & Authors
Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites
Park, Hana; Yoon, Chang Young; Kim, Jin Sook; Kim, Joo-Hwan;
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Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.
Molecular identification;Reynoutria japonica;Reynoutria sachalinensis;SNP;
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