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Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites
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  • Journal title : Korean Journal of Plant Resources
  • Volume 28, Issue 6,  2015, pp.743-751
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2015.28.6.743
 Title & Authors
Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites
Park, Hana; Yoon, Chang Young; Kim, Jin Sook; Kim, Joo-Hwan;
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 Abstract
Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica- specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.
 Keywords
Molecular identification;Reynoutria japonica;Reynoutria sachalinensis;SNP;
 Language
English
 Cited by
 References
1.
Bailey, J.P. and C.A. Stace. 1992. Chromosome number, morphology, pairing, and DNA values of species and hybrids in the genus Fallopia (Polygonaceae). P. Syst. Evol. 180:29-52. crossref(new window)

2.
Bailey, J.P., K. Bimova and B. Mandak. 2007. The potential role of polyploidy and hybridisation in the further evolution of the highly invasive Fallopia taxa in Europe. Ecol. Res. 22(6):920-928. crossref(new window)

3.
Baik, M.C., H.D. Hoang and H. Karl. 1986. A check-list of the Korean cultivated plants. Die Kulturpflanze. 34(1):69-144. crossref(new window)

4.
Batley, J., R. Mogg, D. Edwards, H. Sullivan and K.J. Edwards. 2002. A high-throughput SNuPE assay for genotyping SNPs in the Flanking regions of Zea mays sequence tagged simple sequence repeats. Mol. Breeding 11:111-120.

5.
CBOL Plant Working Group. 2009. A DNA barcode for land plants. PNAS. 106(31):12794-12797. crossref(new window)

6.
Chase, M.W., D.E. Soltis, R.G. Olmstead, D. Morgan, D.H. Les, B.D. Mishler and V.A. Albert. 1993. Phylogenetics of seed plants: an analysis of nucleotide sequences from the plastid gene rbcL. Ann. Mo. Bot. Gard. 80(3):528-580. crossref(new window)

7.
Chase, M.W., S. Zmarzty, M.D. Lledó, K.J. Wurdack, S.M. Swensen and M.F. Fay. 2002. When in doubt, put it in Flacourtiaceae: a molecular phylogenetic analysis based on plastid rbcL DNA sequences. KEW Bulletin 57(1):141-181. crossref(new window)

8.
Ching, A., K.S. Caldwell, M. Jung, M. Dolan, O.S. Smith, S. Tingey, M. Morgante and A.J. Rafalski. 2002. SNP frequency, haplotype structure and linkage disequilibrium in elite maize inbred lines. BMC Genet. 3(1):19.

9.
Chiang, T.Y., B.A. Schaal and C.I. Peng. 1998. Universal primers for amplification and sequencing a noncoding spacer between the atpB and rbcL genes of chloroplast DNA. Bot. Bull. Acad. Sin. 39:245-250.

10.
Chung, J.H., Y.M. Kim, J.P. Bak, I.S. Ryu, K.H. Leem, C.J. Kim and E.H. Kim. 2011. The Effect of Pharmacopuncture with Polygonum cuspidatum Sieb et Zucc. Solution on Collagen-induced Arthritis in Wistar Rats. Korean J. Acupunct. 28(1):79-89.

11.
Conolly, A.P. 1977. The distribution and history in the British Isles of some alien species of Polygonum and Reynoutria. Watsonia 11:291-311.

12.
Deynze, A.V., K. Stoffel, C.R. Buell, A. Kozik, J. Liu, E.V.D. Knaap and D. Francis. 2007. Diversity in conserved genes in tomato. BMC Genet. 8:465. crossref(new window)

13.
Do, Y.J., S.K. Ku, H.T. Kim, T.H. Oh, Y.M. Cho, S.W. Kim, I.S. Ryu and K.W. Lee. 2011. Antiosteoporotic Effects of Polygoni Multiflori Radix (PMR) in Ovariectomized (OVX)-Induced Osteoporosis ddY Mice. J. Vet. Clin. 28(4):375-386.

14.
Dorigo, W., A. Lucieer, T. Podobnikar and A. Carni. 2012. Mapping invasive Fallopia japonica by combined spectral, spatial, and temporal analysis of digital orthophotos. Int. J. Appl. Earth Obs. Geoinf. 19:185-195. crossref(new window)

15.
Doyle, J.J. and J.L. Doyle. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19:11-15.

16.
Fay, M.F., K.M. Cameron, G.T. Prance, M.D. Lledó and M.W. Chase. 1997. Familial relationships of Rhabdodendron (Rhabdodendraceae): plastid rbcL sequences indicate a caryophyllid placement. KEW Bulletin 52(4):923-932. crossref(new window)

17.
Fourmann, M., P. Barret, N. Froger, C. Baron, F. Charlot, R. Delourme and D. Brunel. 2002. From Arabidopsis thaliana to Brassica napus: development of amplified consensus genetic markers (ACGM) for construction of a gene map. Theor. Appl. Genet. 105(8):1196-1206. crossref(new window)

18.
Gammon, M.A. and R. Kesseli. 2010. Haplotypes of Fallopia introduced into the US. Biol. Invasions 12:421-427. crossref(new window)

19.
Hollingsworth, M.L., J.P. Bailey, P.M. Hollingsworth and C. Ferris. 1999. Chloroplast DNA variation and hybridization between invasive populations of Japanese knotweed and giant knotweed (Fallopia, Polygonaceae). Bot. J. Linn. Soc. 129(2):139-154. crossref(new window)

20.
Lahaye, R., V. Savolainen, S. Duthoit, O. Maurin and M.V.D. Bank. 2008. A test of psbK-psbI and atpF-atpH as potential plant DNA barcodes using the flora of the Kruger National Park as a model system (South Africa). Nature Precedings. hdl:10101/npre.2008.1896.1

21.
Lajter, I., I. Zupkó, J. Molnár, G. Jakab, L. Balogh, A. Vasas and J. Hohmann. 2013. Antiproliferative activity of Polygonaceae species from the Carpathian Basin against human cancer cell lines. Phytotherapy Research 27(1):77-85. crossref(new window)

22.
Lee, W.T., W.K. Paik and K.O. Yoo. 1997. Distribution survey on medicinal plants in Kangwon province. Korean J. Plant. Res. 10(4):275-291.

23.
In, J.G., M.K. Kim, O.R. Lee, Y.J. Kim, B.S. Lee, S.Y. Kim, W.S. Kwon and D.C. Yang. 2010. Molecular identification of Korean mountain ginseng using an amplification refractory mutation system (ARMS). J. of Ginseng Res. 34(1):41-46. crossref(new window)

24.
Jaretzky, R. 1925. Beiträge zur Systematik der Polygonaceae unter Berücksichtigung des Oxymethy lanthrachinon‐Vorkommens. Repertorium Novarum Specierum Regni Vegetabilis 22(4‐12):49-83 (in German). crossref(new window)

25.
Kim, J.M., D.S. Cha, S.R. Jeon, H. Jeon, J.P. Lim, H. Choi, K.J. Lee, M.S. Kang, H.J. Na, M.Y. Kim, K.H. Leem, H.C. Kim and Y.M. Bu. 2008. Neuroprotective effect of the roots of Polygonum cuspidatum on transient focal cerebral ischemia in rats. Kor. J. Herbology 23(2):225-233.

26.
Kim, J.S., H.W. Jang, J.S. Kim, H.J. Kim and J.H. Kim. 2012. Molecular identification of Schisandra chinensis and its allied species using multiplex PCR based on SNPs. Gene & Genomics 34(3):283-290. crossref(new window)

27.
Kim, J.Y. 2013. Development and industrialization of functional bioactive material from the medicinal plan. J. Korean Soc. Food Sci. Nutr. 18(2):1-6.

28.
Kim, J.Y. and C.W. Park, 2000. Morphological and chromosomal variation in Fallopia section Reynoutria (Polygonaceae) in Korea. Brittonia 52(1):34-48. crossref(new window)

29.
Kim, M.K., H. Wang, Y.J. Kim, S. Sathiyamoorthy, W.S. Kwon and D.C. Yang. 2013. Molecular authentication by multiplex-PCR of three similar medicinal plant species: Cynanchum wilfordii, Cynanchum auriculatum and Polygonum multiflorum (Fallopia multiflorum). J. Med. Plants Res. 7(35):2584-2589.

30.
Kim, S.B. and A. Misra. 2007. SNP Genotyping: Technologies and Biomedical Applications. Annu. Rev. Biomed. Eng. 9:289-320. crossref(new window)

31.
Kim, Y.W. and Lee S.H. 2013. Current status of medicinal herbs recorded from the records in Gongju City, Chungcheongnam Province, Korea. Kor. Herb. Med. Inf. 1:77.

32.
Park, J.H., H.K. Moon and C.W. Park, 2011. Flavonoid chemistry of Fallopia sect. Reynoutria (Polygonaceae) in Korea. Korean J. Pl. Taxon. 41(1):10-15.

33.
Schuster, T.M., K.L. Wilson and K.A. Kron. 2011. Phylogenetic relationships of Muehlenbeckia, Fallopia, and Reynoutria (Polygonaceae) investigated with chloroplast and nuclear sequence data. Int. J. Plant Sci. 172(8):1053-1066. crossref(new window)

34.
Shaw, J., E.B. Lichey, E.E. Schilling and R.L. Small. 2007. Comparison of whole chloroplast genome sequences to shoose noncoding regions for phylogenetic studies in angiosperms: The tortoise and the hare III. Am. J. Bot. 94(3):275-288. crossref(new window)

35.
Sohn, H.Y., E.J. Kum, Y.S. Kwon, G.S. Kwon, I.G. Jin, H.Y. Kwon, C.S. Kwon and K.H. Son. 2003. Screening of anti-candidiosis agent from medicinal and wild plants. Kor. J. Life Sci. 13(5):604-617. crossref(new window)

36.
Steward, A.N. 1930. The polygoneae of eastern Asia. Contr. Gray Herb. 5(88):1-133.

37.
Stoll, P., K. Gatzsch, H.P. Rusterholz and B. Baur. 2012. Response of plant and gastropod species to knotweed invasion. Basic Appl. Ecol. 13:232-240. crossref(new window)

38.
Taberlet, P., L. Gielly, G. Pautou and J. Bouvet. 1991. Universal primers for amplification of tree non-coding regions of chloroplast DNA. Plant Mol. Biol. 17:1105-1109. crossref(new window)

39.
Yan, P., Q.H. Pang, X.W. Jiao, A. Zhao, Y.J. Shen and S.J. Zhao. 2008. Genetic variation and identification of cultivated Fallopia multiflora and its wild relative by using chloroplast matK and 18S rRNA gene sequences. Planta Med. 74:1504-1509. crossref(new window)