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Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)
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  • Journal title : Korean Journal of Plant Resources
  • Volume 29, Issue 1,  2016, pp.143-153
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2016.29.1.143
 Title & Authors
Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)
Kim, Dong-Gun; Kantayos, Vipada; Kim, Dong-Kwan; Park, Heung-Gyu; Kim, Haeng-Hoon; Rha, Eui-Shik; Lee, Sheong Chun; Bae, Chang-Hyu;
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 Abstract
Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.
 Keywords
Callus;Organogenesis;Somatic embryogenesis;Soybean;TDZ;
 Language
Korean
 Cited by
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