Advanced SearchSearch Tips
Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : Korean Journal of Plant Resources
  • Volume 29, Issue 1,  2016, pp.143-153
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2016.29.1.143
 Title & Authors
Plant Regeneration by in vitro Tissue Culture in Korean Soybean (Glycine max L.)
Kim, Dong-Gun; Kantayos, Vipada; Kim, Dong-Kwan; Park, Heung-Gyu; Kim, Haeng-Hoon; Rha, Eui-Shik; Lee, Sheong Chun; Bae, Chang-Hyu;
  PDF(new window)
Plant regeneration via organogenesis and somatic embryogenesis was investigated in Korean soybean cultivars including Cheongja 3, Jinpumkong 2, Taekwangkong and Uram. Cotyledon, cotyledon+hypocotyl and hypocotyl segments of 7-day-old seedlings were cultured on MS medium containing various concentration (0, 1, 2 and 4 ㎎/L) of BA and TDZ. The results showed that MS medium supplemented with BA 2.0 ㎎/L yielded the highest shoot formation ratio of 83.3%. In 4 cultivars, Taekwangkong showed the highest ratio of shoot formation. When various sizes of immature cotyledons (S: 1∼ 2 ㎜, M: 3∼5 ㎜, L: 6∼8 ㎜) were tested on MS medium containing 2,4-D 40 ㎎/L for somatic embryogenesis, the optimum size for embryogenic callus induction was 3∼5 ㎜ in length of immature cotyledons. In 4 cultivars, Taekwangkong showed the highest percentage of embryogenic callus induction. The results indicate that Taekwangkong is the best soybean cultivar for plant regeneration via organogenesis and embryogenic callus induction among the 4 cultivars.
Callus;Organogenesis;Somatic embryogenesis;Soybean;TDZ;
 Cited by
Arun, M., K. Subramanyam, J. Theboral, A. Ganapathi and M. Manickavasagam. 2014. Optimized shoot regeneration for Indian soybean: the influence of exogenous polymines. Plant Cell. Tiss. Organ Cult. 117:305-309. crossref(new window)

Bailey, M.A., H.R. Boerma and W.A. Parrott. 1993. Genotype effects on proliferative embryogenesis and plant regeneration of soybean. Cell Dev. Biol. Plant 29:102-108. crossref(new window)

Bean, S.J., P.S. Gooding, P.M. Mullineaux and D.R. Davies. 1997. A simple system for pea transformation. Plant Cell Rep. 16:513-519. crossref(new window)

Bhagwat, B., L.G.E. Vieira and L.R. Ericson. 1996. Stimulation of in vitro shoot proliferation from nodal explants of cassava by thidiazuron, benzyladenine and gibberellic acid. Plant Cell Tiss. Organ Cult. 46:1-7. crossref(new window)

Dan, Y. and N.A. Reichert. 1998. Organogenic regeneration of soybean from hypocotyl explants. In Vitro Cell Dev. Biol. Plant. 34:14-21.

Finer, J.J. and A. Nagasawa. 1988. Development of an embryogenic suspension culture of soybean (Glycine max Merill). Plant Cell Tissue. Organ Cult. 15:125-136. crossref(new window)

Franklin, G., L. Carpenter, E. Davis, C.S. Reddy, D. Al-Abed, W.A. Alaiwi, M. Parani, B. Smith, S.L. Goldman and R.V. Sairam. 2004. Factors influencing regeneration of soybean from mature and immature cotyledons. Plant Growth Regul. 43:73-79. crossref(new window)

Gamborg, O.L., R.A. Miller and K. Ojima. 1968. Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell. Res. 50:151-158. crossref(new window)

Hiraga, S., H. Minakawa, K. Takahashi, R. Takahashi, M. Hajika, K. Harad and N. Ohtsubo. 2007. Evaluation of somatic embryogenesis from immature cotyledons of Japanese soybean cultivars. Plant Biotechnol. 24:435-440. crossref(new window)

Hofmann, N., L.N. Randall and S.S. Korban. 2004. Influence of media components and pH on somatic embryogensis induction in three genotypes soybean. Plant Cell Tiss. Organ Cult. 77:157-163. crossref(new window)

Joyner, E.Y., L.S. Boykin and M.A. Lodhi. 2010. Callus induction and organogenesis in soybean [Glycine max (L.) Merr.] cv. pyramid from mature cotyledons and embryos. The Open Plant Sci. J. 4:18-21. crossref(new window)

Kaneda, Y., Y. Tabei, S. Nishimura, K. Harada, T. Akihama and K. Kitamura. 1997. Combination of thidiazuron and basal media with low salt concentrations increases the frequency of shoot organogenesis in soybeans [Glycine max(L.) Merr.]. Plant Cell Rep. 17:8-12. crossref(new window)

Kim, D-g. 2016. Regeneration ability of Korean soybean (Glycine max L.) cultivars by in vitro tissue culture. Department of Life Resources, M.S. Thesis, Sunchon National Univ., Korea. pp. 1-40 (in Korean).

Kim, K.H., H.K. Park, M.S. Park and U.D. Yeo. 2001. Effects of auxins and cytokinins on organogenesis of soybean Glycine max L. J. Plant Biol. 3:95-100.

Kim, M.-J. and K.-S. Kim. 2005. Functional and chemical composition of Hwanggumkong, Yakong and Huktae. Korean J. Food Coookery Sci. 6:844-850 (in Korean).

Kim, Y.H., H.M. Park, M.S. Choi, S.I. Sohn, D.B. Shin and J.Y. Lee. 2008. Efficient transformation method of soybean using meristemic tissues of germinating seeds. Korean J. Breed. Sci. 40(3):278-285 (in Korean).

Kita, Y., K. Nishizawa, M. Takahashi, M. Kitayama and M. Ishimoto. 2007. Genetic improvement of the somatic embryogenesis and regeneration in soybean and transformation of the improved breeding lines. Plant Cell Rep. 26:439-447. crossref(new window)

Klink, V.P., M.H. MacDonald, V.E. Martins, S.C. Park, K.H. Kim, S.H. Baek and B.F. Matthews. 2008. A new diminutive Glycine max genotype with a rapid life cycle, embryogenic potential and transformation capabilities. Plant Cell Tiss. Organ Cult. 92:183-195. crossref(new window)

Ko, T.S. and S.S. Korban. 2004. Enhancing the frequency of somatic embryogenesis following Agrobacterium-mediated transformation of immature cotyledons of soybean [Glycine max (L.) Merrill.]. In Vitro Cell Dev. Biol. Plant. 40:552-558. crossref(new window)

Komatsuda, T. and K. Ohyama. 1988. Genotypes of high competence for somatic embryogenesis and plant regeneration in soybean Glycine max. Theor. Appl. Genet. 75:695-700.

Kwon, S.J., K.Y. Cho and H.H. Kim. 2014. Medium composition and growth regulator on organogenesis Platycodon grandiflorum (Jacq.) A. D.C with yellow green petals. Korean J. Plant Res. 27(1):43-50 (in Korean). crossref(new window)

Lazzeri, P.A., D.F. Hildebrand and G.B. Collins. 1987. Soybean somatic embryogenesis effect of nutritional, physical and chemical factors. Plant Mol. Biol. Rep. 10:209-220.

Lim, H.S., T.S. Ko, K.N. Lambert, H.G. Kim, S.S. Korban, G.L. Hartman and L.L. Domier. 2005. Soybean mosaic virus helper component-protease enhances somatic embryo production and stabilizes transgene expression in soybean. Plant Physiol. Biochem. 43:1014-1021. crossref(new window)

Liu, Q.Q., G. Chen, J.Y. Gai, Y.L. Zhu, L.F. Yang, G.P. Wei and C. Wang. 2010. Highly efficient shoot regeneration from cotyledonary nodes of vegetable soybean. Kor. J. Hort. Sci. Technol. 28:307-313.

Ma, X.-H. and T.-L. Wu. 2008. Rapid and efficient regeneration in soybean〔Glycine max (L.) Merrill〕from whole cotyledonary node explants. Acta Physiol. Plant 30:209-216. crossref(new window)

Malik, K.A. and P.K. Saxena. 1992. Regeneration in Phaseolus vulgaris L. High frequency induction of direct shoot fromation in intact seeding by benzylaminopurine and thidiazuron. Planta 186:384-389.

Mante, S., R. Scorza and J. Cordts. 1989. A simple, rapid protocol for adventitious shoot development from mature cotyledons of Glycine max cv. Bragg. In Vitro Cell. Dev. Biol. 25:385-388. crossref(new window)

Marele, D. 2013. Influence of kinetin on organogenesis of soybean in vitro. Zootehnie si Technologii de Industrie Alimentara 9:163-166.

Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15:473-497. crossref(new window)

Rajasekaran, K. and J.W. Pellow. 1997. Somatic embryogenesis from cultured epicotyls and primary leaves of soybean [Glycine max (L.) Merrill]. In Vitro Cell Dev. Bio. Plant. 33:88-91.

Sairam, R.V., G. Franklin, R. Hassel, B. Smith, K. Meeker, N. Kashikar, M. Parani, D. Al. Abed, S. Ismail, K. Berry and S.L. Goldman. 2003. A study on the effect of genotypes, plant growth regulators and sugars in promoting plant regeneration via organogenesis from soybean cotyledonary nodal callus. Plant Cell Tiss. Organ Cult. 75:79-85. crossref(new window)

Saka, H., T.H. Voqui-Dinh and T-Y Cheng. 1980. Stimulation of multiple shoot formation on soybean stem nodes in culture. Plant Sci. Lett. 19:193-201. crossref(new window)

Santarém, E.R., B. Pelissier and J.J. Finer. 1997. Effect of explant orientation, pH, solidifying agent and wounding on initiation of soybean somatic embryo. In Vitro Cell Dev. Bio. Plant. 33:13-19. crossref(new window)

Sellars, R.M., G.M. Southward and G.C. Phillips. 1990. Adventitious somatic embryogenesis from cultured immature zygotic embryos of peanut and soybean. Crop Sci. 30:408-414. crossref(new window)

Shan, Z, K. Raemakers, E.N. Tzitzikas, Z. Ma and R.G.H. Visser. 2005. Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr). Plant Cell Rep. 24:507-512. crossref(new window)

Verma, K., A. Rani and R. Saini. 2011. An efficient plant regeneration system from half seed explants of soybean [Glycine max (L.) Merrill] using thidiazuron. Soybean Res. 8:12-23.

White, D.W.R. and C. Voisey. 1994. Prolific direct plant regeneration from cotyledons of white clover. Plant Cell Rep. 13:303-308.

Wright, M.S., M.H. Williams, P.E. Pierson and M.G. Carns. 1987. Initiation and propagation of Glycine max L. Merr. plants from tissue-cultured epicotyls. Plant Cell Tiss. Organ Cult. 8:83-90. crossref(new window)

Yang, C., T. Zhao, D. Yu and J. Gai. 2009. Somatic embryogenesis and plant regeneration in Chinese soybean (Glycine max (L.) Merr.) - impacts of mannitol, abscisic acid, and explant age. In Vitro Cell Dev. Biol. Plant. 45:180-188. crossref(new window)

Yoshida, T. 2002. Adventitious shoot formation from hypocotyl sections of mature soybean seeds. Breed. Sci. 52:1-8. crossref(new window)

Zia, M., Z.F. Rizvi, R-U. Rehman and M.F. Chaudhary. 2010. Short communication micropropagation of two Pakistani soybean (Glycine max L.) cultivars from cotyledonary nodes. Spanish Journal of Agricultural Res. 8:448-453. crossref(new window)

김종홍, 배창휴. 1992. 대두자엽조직의 분화에 미치는 2,4-D와 BA의 영향. 순천대학 기초과학연구. 3:47-57.

송순영. 2010. 희귀 및 멸종위기 식물 만년콩 (Euchresta japonica Benth.)의 종자 발아 및 기내증식에 관한 연구. 제주대학교 석사학위논문.

하건수, 한태정. 2002. 대두 품종에 따른 자엽절에서의 다신초 형성. Plant Biotechnology. 29:51-57. crossref(new window)