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Activating Transcription Factor 3 is a Molecular Target for Apoptotic Effect of Silymarin in Human Colorectal Cancer Cells
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  • Journal title : Korean Journal of Plant Resources
  • Volume 29, Issue 3,  2016, pp.281-288
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2016.29.3.281
 Title & Authors
Activating Transcription Factor 3 is a Molecular Target for Apoptotic Effect of Silymarin in Human Colorectal Cancer Cells
Eo, Hyun Ji; Park, Gwang Hun; Jeong, Jin Boo;
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 Abstract
Apoptosis has been regarded as a therapeutic target because apoptosis is typically disturbed in human cancer. Silymarin found in the seeds of the milk thistle (Silybum marianum) has been reported to exert anti-cancer properties through apoptosis. This study was performed to investigate the molecular target for silymarin-mediated apoptosis in human colorectal cancer cells. Silymarin reduced the cell viability and induced an apoptosis in human colorectal cancer cells. ATF3 overexpression increased PARP cleavage by silymarin. Increased ATF3 expression in both protein and mRNA was observed in silymarin-treated cells. In addition, silymarin increased the luciferase activity of ATF3 promoter. Inhibition of JNK and IκK-α blocked silymarin-mediated ATF3 expression. The results suggest that silymarin induces apoptosis through JNK and IκKα-dependent ATF3 expression in human colorectal cancer cells.
 Keywords
Silymarin;Activating transcription factor 3;Apoptosis;Human colorectal cancer;Cancer chemoprevention;
 Language
English
 Cited by
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