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Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata
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  • Journal title : Korean Journal of Plant Resources
  • Volume 29, Issue 3,  2016, pp.347-354
  • Publisher : The Plant Resources Society of Korea
  • DOI : 10.7732/kjpr.2016.29.3.347
 Title & Authors
Effect of Colchicine on Chromosome Doubling in Codonopsis lanceolata
Kwon, Soo-Jeong; Seo, Dong-Yeon; Cho, Gab-Yeon; Lee, Moon-Soon; Moon, Young-Ja; Boo, Hee-Ock; Woo, Sun-Hee; Kim, Hag-Hyun;
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The present study was performed to investigate the effects of the colchicine concentrations on chromosome doubling for producing of tetraploid plants of Codonopsis lanceolata, and its effect on plant morphology. A total of 180 individuals germinated from 16 treatment groups, were exposed to various concentrations (0.05-1.0% w/v) of colchicine for different soaking duration (3-24 hour). The highest numbers of tetraploid plants (3) were observed from the lowest concentration of colchicine (0.05%), and one (1) tetraploid plant was obtained from the 0.5% concentration group with a 6 hour treatment. However, no tetraploid individual was observed in any other treatment groups. The plant height of the diploid (18.1 ㎝) was slightly shorter than that of the tetraploid (13.4 ㎝). The fresh weight of the main root in the diploid (0.5 g) was four-fold higher than the tetraploid (2.2 g). The colchicine-treated plant regeneration rate in C. lanceolata was decreased when the plants were subjected to high concentration of colchicine. In particular, the highest number of tetraploid plants (5 and 3) was obtained from the lower concentration (0.05% and 0.1%) of colchicine for 6-hour treatment, which were a higher rate (29.4% and 30%) of regenerated tetraploid plants than other regenerated plants. As in the seed treatment result, the plant height of the diploid was significantly higher (10.4 ㎝) than tetraploid. The higher morphological changes were observed comparatively from tetraploid plants than the diploid.
Concentration;Soaking duration;Explant;DNA content;Morphological changes;
 Cited by
Aina, O., K. Quesenberry and M. Gallo. 2012. In vitro induction of tetraploids in Arachis paraguariensis. Plant Cell Tiss. Org. 111(2):231-238. crossref(new window)

Bakry, F., N.P. de la Reberdiere, S. Pichot and C. Jenny. 2007. In liquid medium colchicine treatment induces non chimerical doubled-diploids in a wide range of mono-and interspecific diploid banana clones. Fruits 62(1):3-12. crossref(new window)

Chang, H.G., J.H. Bae, D.T. Lee, S.K. Chun and J.G. Kim. 1987. Mineral constituents of honey produced in Korea. Korean J. Food Sci. Technol. 19(5):426-428.

Cockerham, L.E. and G.J. Galletta. 1976. A survey of pollen characteristics in certain Vaccinium species. J. Amer. Soc. Hort. Sci. 101(6):671-676.

Dhooghe, E., K. Van Laere, T. Eeckhaut, L. Leus and J. Van Huylenbroeck. 2011. Mitotic chromosome doubling of plant tissues in vitro. Plant Cell Tiss. Org. 104(3):359-373. crossref(new window)

Eenink A.H. and J.M. Alvarez. 1975. Indirect selection for tetraploidy in lettuce (Lactuca sativa L.). Euphytica 24(3):661-668. crossref(new window)

Esen, A., K.S. Rebert and G. Giuseppe. 1978. Seed set, size, and development after 4x × 2x and 4x × 4x crosses in Citrus. Euphytica 27(1):238-294.

Gao, S.L., D.N. Zhu, Z.H. Cai and D.R. Xu. 1996. Autotetraploid plants from colchicine-treated bud culture of Salvia miltiorrhiza Bge. Plant Cell Tiss. Org. 47(1):73-77. crossref(new window)

Grouh, M.S.H., H. Meftahizade, N. Lotfi, V. Rahimi and B. Baniasadi. 2011. Doubling the chromosome number of Salvia hains using colchicine: Evaluation of morphological traits of recovered plants. J. Med. Plants Res. 5(19):4892-4898.

Hadlaczky, G., G. Bistrary, T. Parznovszky and D. Dudits. 1983. Mass isolation of plant chromosomes and nuclei. Planta 157(3):278-385. crossref(new window)

Hahn, S.J. 1969. Studies on the tetraploid radish (Raphanum Sativus L.) induced by colchicine. On the resistance the virus diseases. J. Kor. Soc. Hort. Sci. 5(1):48-56 (in Korean).

Hancock, J. F. 1997. The colchicine story. HortScience 32(6): 1011-1012.

Kehr, A.E. 1996. Woody plant polyploidy. American Nurseryman 183:40-48.

Kim, I.H., H.H Kim, E.Y. Hong, J.S. Yun, T.Y. J.K. Hwang and C.H. Lee. 2003. Breeding of tetraploid in Platycodon grandiflorum (Jacq.) A. DC. by colchicine treatment. Plant Resources 6(3):188-194.

Kwon, S.J., G.Y. Cho and H.H. Kim. 2013. A tetraploid induction in Hypericum patulum Thunberg by colchicine soaking treatment. Korean J. Plant Res. 26(2):284-288 (in Korean). crossref(new window)

Kwon, S.J., S.K. Roy, K.Y. Cho, Y.J. Moon, S.H. Woo and H.H. Kim. 2015. The effective approach to induce tetraploid plant of Prunella vulgaris for. albiflora Nakai. Korean J. Crop Sci. 60(1):107-113. crossref(new window)

Lapin, K.O. 1975. Polyploidy and mutations induced in apricot by colchicine treatment. Can. J. Genet. Cytol. 17(4):591-599. crossref(new window)

Moghbel, N., M.K. Borujeni and F. Bernard. 2015. Colchicine effect on the DNA content and stomata size of Glycyrrhiza glabra var. glandulifera and Carthamus tinctorius L. cultured in vitro. J. Gen. Eng. Biotechnol. 13(1):1-6. crossref(new window)

Moon, K.S. 1984. Components of medicinal plant and their use. Ilwol Pub Co., Seoul, Korea. pp. 586-587.

Nilanthi, D., X.L.Chen, F.C. Zhao, Y.S. Yang and H. Wu. 2009. Induction of tetraploids from petiole explants through colchicine treatments in Echinacea purpurea L. BioMed Research International 2009.

Oh, S. A, K.H. Min, Y.S. Choi, S.B. Park, Y,C, Kim and S.M. Cho. 2015. Development of tetraploid watermelon using chromosome doubling reagent treatments. Korean J. Plant Res. 28(5):656-664 (in Korean). crossref(new window)

Park, K.J. 1994. Cold-hardiness tetraploid induced by colchicine treatment in mulberry seedlings (Morus alba L. Yongchonppong/Kaeryanppong). Korean J. Seric. Sci. 36(1):1-7 (in Korean).

Sourour, A., B. Ameni and C. Mejda. 2014. Efficient production of tetraploid barley (Hordeum vulgare L.) by colchicine treatment of diploid barley. J. Exp. Biol. 2(1S):113-119.

Suh, J.T., K.S. Cho, M.H. Yang and S.Y. Hong. 2005. Characteristics of mutant by colchicine treatment of wild Lilium leichtlinii seed native to Korea. Kor. J. Hort. Sci. Technol. 23(4):430-432 (in Korean).

Tan, G.Y. and G.M. Dunn. 1973. Relationship of stomatal length and frequency and pollen-grain diameter to ploidy level in Bromus inermis Leyss. Crop Sci. 13(3):332-334. crossref(new window)

Thao, N.T.P., K. Ureshino, I. Miyajima, Y. Ozaki and H. Okubo. 2003. Induction of tetraploids in ornamental alocasia through colchicine and oryzalin treatments. Plant Cell Tiss. Org. 72(1):19-25. crossref(new window)

Wu, Y., F. Yang, X. Zhao and W. Yang. 2011. Identification of tetraploid mutants of Platycodon grandiflorus by colchicine induction. Caryologia 64(3):343-349. crossref(new window)