JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Phytoplankton as Standard Test Species for Marine Ecotoxicological Evaluation
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Phytoplankton as Standard Test Species for Marine Ecotoxicological Evaluation
Park Gyung-Soo; Lee Sang-Hee; Lee Seung-Min;
  PDF(new window)
 Abstract
A series of experiments was conducted to identify the potential of five phytoplankton species as standard test species for marine ecotoxicological tests. The candidate phytoplankton species are Skeletonema costatum, Heterosigma akashiwo, Prorocentrum micans, Isochrysis galbana, and Tetraselmis suecica. Salinity tolerance and sensitivity on potassium dichromate as a reference material were identified. Toxicity of eleven ocean dumped sewage sludges and four red tide expellent extracts were estimated by the inhibition of population growth rates (PGR) of marine diatom S. costatum, While most species revealed relatively weak tolerance on salinity, T. suecica demonstrated the highest salinity tolerance ranged from psu and the others psu. H. akashiwo revealed the highest sensitivity as 72h =0.76mg/L and T. suecica the lowest as 72h =8.89mg/L on potassium dichromate. Sludge extracts from industrial waste, domestic sewage and livestock farm waste sludge showed high toxicity as 72h < and lowest toxicity from filtration bed sludge as 72h = NOEC (No Observed Effective Concentration) of sludge extract ranged from < to and this indicated high phytotoxicity of ocean dumped sewage sludge. The test sensitivity of phytoplankton PGR inhibition was much higher than those of marine rotifer Brachionus plicatilis mortality test and bioluminescent inhibition test by marine bacteria Vibrio fischeri, and comparable with the sea urchin (Strongylocentrotus intermedius) fertilization test. As a result the phytotoxicity test using phytoplankton PGR inhibition () must be a useful tool for marine phyto-toxicological evaluation of ocean dumped materials.
 Keywords
Phytoplankton;Ecotoxicity;Bioassay;Salinity;tolerance;Sewage sludge;Skeletonema costatum;Heterosigma akashiwo;Prorocentrum micans;
 Language
Korean
 Cited by
1.
식물플랑크톤의 개체군성장저해율을 이용한 해양생태독성 시험방법에 관한 연구,이승민;박경수;안경호;박승윤;이상희;

한국해양학회지:바다, 2008. vol.13. 2, pp.112-120
2.
해양생태독성평가를 위한 표준시험방법 개발에 관한 연구,박경수;이승민;한태준;이정석;

한국해양학회지:바다, 2008. vol.13. 2, pp.106-111
3.
해양성 발광박테리아를 이용한 해양환경 독성평가 시험법 개발: N-Tox test,이규태;박경수;김평중;

한국해양학회지:바다, 2008. vol.13. 2, pp.156-163
4.
구리, 카드뮴, 펜벤다졸, 설파티아졸이 국내산 풍년새우 생존에 미치는 영향,문성대;조창현;곽인실;이창훈;

Environmental health and toxicology, 2009. vol.24. 4, pp.311-320
5.
염분과 표준 독성물질(Cu, Cd)이 말똥성게(Hemicentrotus pulcherrimus)의 수정 및 배 발생률에 미치는 영향,황운기;이충원;김광섭;안경호;박승윤;

Environmental health and toxicology, 2009. vol.24. 1, pp.9-16
6.
Genetic Relationships among Multiple Strains of the Genus Tetraselmis Based on Partial 18S rDNA Sequences,;;

ALGAE, 2009. vol.24. 4, pp.205-212 crossref(new window)
7.
해양생태독성 평가용 표준시험생물로서 섬모충류 Mesodinium rubrum에 대한 연구,안경호;박경수;이승민;

Journal of Environmental Science International, 2011. vol.20. 9, pp.1087-1093 crossref(new window)
 References
1.
Chapman, P. M., 1992, Pollution status of North Sea sediments- an international integrative study, Mar. Ecol. Prog. Ser., 91, 313-322 crossref(new window)

2.
Ghirardini, A. V., T. Birkemeyer, A. A. Novelli, E. Delaney, B. Pavoni and P. F. Ghetti, 1999, An integrated approach to sediment quality assessment: the Venetian lagoon as a case study, Aquat. Ecosyst. Health Manage., 2, 435-447 crossref(new window)

3.
Anderson, B. S., J. W. Hunt, B. M. Phillips, R. Fairey, C. A. Roberts, J. M. Oakden, H. M. Puckett, M. Stephenson, R. S. Tjeerdema, E. R. Long, C. J. Wilson and J. M. Lyons, 2001, Sediment quality in Los Angeles Harbor, USA: A triad assessment, Environ. Toxicol. Chem., 20, 359-370 crossref(new window)

4.
Silvia, S., A. Re, P. Pestana, A. Rodrigues and V. Quintino, 2004, Sediment disturbance off the Tagus Estuary, Western Portugal: chronic contamination, sewage outfall operation and runoff events, Mar. Pollut. Bull., 49, 154-162 crossref(new window)

5.
Rand, G. M. and S. R. Petrocelli, 1985, Fundamentals of Aquatic Toxicology, Hemisphere Publishing Corporation, Washington, 666pp

6.
Lee, J. S., K. T. Lee, D. H. Kim, C. K. Kim, J. H. Lee, K. H. Park and G. S. Park, 2005a, Application of indigenous benthic amphipods as sediment toxicity testing organisms, Ocean Science Journal, 40, 17-24

7.
Lee, J. S., K. T. Lee and G. S. Park, 2005b, Acute toxicity of heavy metal tributyltin, ammonia and polycyclic aromatic hydrocarbons to benthic amphipod Grandidierella japonica, Ocean Science Journal, 40, 61-66 crossref(new window)

8.
Park, G. S., C. S. Chung, S. H. Lee, G. H. Hong, S. H. Kim, S. Y. Park, S. J. Yoon and S. M. Lee, 2005, Ecotoxicological evaluation of sewage sludge using bioluminescent marine bacteria and rotifer, Ocean Science Journal, 40, 91-100 crossref(new window)

9.
Burton, G. A., 1992, Sediment Toxicity Assessment, Lewis Publishers Inc., Chelsea, 457pp

10.
ISO, 1995, Water quality- marine algal growth inhibition test with Skeletonema costatum and Phaeodactylum tricomutum. the International Organization for Standardization, ISO 10253, 7pp

11.
NIWA, 1998, Marine Algae (Dunaliella tertiolecta) Chronic Toxicity Test Protocol, National Institute of Water and Atmospheric Research, New Zealand, 30pp

12.
APHA, AWWA and WEF, 1995, Standard Methods for the Examination of Water and Wastewater, American Public Health Association, American Water Works Association, Water Environment Federation, Washington, D.C., pp. 1-47

13.
Hooten, R. L. and R. S. Carr, 1997, Development and application of a marine sediment pore-water toxicity test using Ulva fasciata zoospores, Environ. Toxicol. Chem., 17, 932-940 crossref(new window)

14.
Cheung, Y. H., A. Neller, K. H. Chu, N. F. Y. Tam, C. K. Wong, Y. S. Wong and M. H. Wong, 1997, Assessment of sediment toxicity using different trophic organisms, Arch. Environ. Contam. Toxicol., 32, 260-267 crossref(new window)

15.
Wong, Y. S., N. F. Y. Tam, P. S. Lau and X. Z. Xue, 1995, The toxicity of marine sediments in Victoria Harbour, Hong Kong, Mar. Pollut. Bull., 31, 4-12

16.
Weideborg, M., E. A. Vik, G. D. Ofjord and O. Kjonno, 1997, Comparison of three marine screening tests and four Oslo and Paris commission procedures to evaluate toxicity of offshore chemicals, Environ. Toxicol. Chem., 16, 384-389 crossref(new window)

17.
Rao, M. U. and V. Mohanchand, 1990, Toxicity of zinc smelter wastes to some marine diatoms, Indian J. Marine Sciences, 19, 181-186

18.
Walsh, G. E. and S. V. Alexander, 1980, A marine algal bioassay method: results with pesticides and industrial waste, Water Air Soil Pollut., 13, 45-55 crossref(new window)

19.
Overnell, J., 1976, Inhibition of marine algal photosynthesis by heavy metals, Mar. Biol., 38, 335-342 crossref(new window)

20.
Jensen, A., B. Rystad and S. Melsom, 1974, Heavy metal tolerance of marine phytoplankton. 1. the tolerance of three algal species to zinc in coastal sea water, J. Exp. Mar. Biol. Ecol., 15, 145-157 crossref(new window)

21.
문성기, 최철만, 2003, 국내 해양식물플랑크톤의 주요종과 분포에 대한 조사, 한국환경과학회, 12, 725-733

22.
환경교육연구회, 2000, 환경오염공정시험법(폐기물 토양오염분야), 대학서림, 564pp

23.
Shapiro, S. S. and M. B. Wilk, 1965, An analysis of variance test for normality (complete samples), Biometrika, 52, 591-611 crossref(new window)

24.
Snedecor, G. W. and W. G. Cochran, 1989, Statistical Methods, 8th Edition, Iowa State University Press, 502pp

25.
Dunnett, C. W., 1964, New table for multiple comparisons with a control, Biometrics, 20, 482 crossref(new window)

26.
Ravail, B. and J. M. Robert, 1985, Influence of salinity on the growth of S. costatum (Greville) Cleve in the waters of the Loire Estuary, Cryptogramie: Algol., 6, 51-60

27.
Shimura, S., H. Shibuya and S. Ichimura, 1979, Growth and photosynthesis properties of some planktonic marine diatoms at various salinity regimes, Umi/la mer, Tokyo, 17(3), 149-155

28.
Chapman, D. V., 1985, Preliminary observations on the interaction between plankton and sewage dumped at sea, Marine Environmental Quality Committee, Int. Co. Explor. Sea CM 1985/E, 26, 11pp

29.
Santoro, E. D. and T. J. Fikslin, 1987, Chemical and toxicological characteristics of sewage sludge ocean dumped in the New York Bight, Mar. Pollut. Bull., 18, 394-399 crossref(new window)

30.
Costello, M. J. and P. Read, 1994, Toxicity of sewage sludge to marine organisms: a review, Mar. Environ. Res., 37, 23-46 crossref(new window)

31.
해양수산부, 2005, 폐기물 해양배출 종합관리 시스템 구축(I), 해양수산부, 1057pp

32.
MOMAF, 2004, Development of Assessment Framework on the Sewage Sludge for Ocean Disposal, Ministry of Maritime Affairs and Fisheries, Korea, 390pp