Advanced SearchSearch Tips
Effects of Ultraviolet-B Radiation on Growth and Photosynthesis in Cucumber Primary Leaves
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of Ultraviolet-B Radiation on Growth and Photosynthesis in Cucumber Primary Leaves
Kim, Hyo-Jin; Kim, Tae-Yun; Hong, Jung-Hee;
  PDF(new window)
In the present study we studied the growth, photosynthetic traits and protective mechanisms against oxidative stress in the primary loaves of cucumber (Cucumis sativus L.) seedlings with or without UV-B treatment. Cucumber seedings were irradiated with UV-B for 10 days in environment-controlled growth chambers. The primary leaves irradiated with UV-B showed reduction in leaf length and decreased biomass production. The reduced biomass production seemed to be due to a negative effect of UV-B radiation on the photosynthetic process. Changes in chemical properties of leaf, such as chi a/b ratio affected photosynthesis. UV-B significantly affected chl b content compared with chi a in the light harvesting complex resulting reduced photosynthetic activity Fv/Fm decreased with an UV-B stress, suggesting that the photosynthetic apparatus, and particularly, PS II was damaged under UV-B stress. Malondialdehyde(MDA) concentration which represents the state of membrane lipid peroxidation Increased significantly under UV-B stress confirming an oxidative stress. UV-B exposure with SA solution(0.1-1.0 mM) can partially ameliorated some of the detrimental effects of UV-B stress. Leaf injuries including loss of chlorophyll and decreased ratio of Fv/Fm were reduced with combined application of UV-B and SA. ABA and JA showed similar mode of action in physiological effects on photosynthetic activities though the levels were lower than those from SA treated plants. Chloroplast ultrastructure was also affected by UV-B exposure. The thickness of leaf tissue components decreased and the number of grana and thylakoids was reduced in chloroplast applied UV-B or SA alone. At combined stress granal and stromal thylakoids were less affected. The leaves under combined stress acquired a significant tolerance to oxidative stress. From these results, it can be suggested that SA may have involved a protective role against UV-B induced oxidative damage.
Biomass production;Chlorophyll fluorescence;Primary leaf;Cucumber(Cucumis sativus L.);Malondialdehyde;Salicylic acid;UV-B;
 Cited by
Madronich, S., R. L. Mckencie, L. O. Bjorn and M. M. Caldwell, 1998, Changes in biologically ultraviolet radiation reaching in Earth's surface, J. Photochem. Photobiol. B. Biol., 46, 5-19 crossref(new window)

Britt, A. B., 1996, DNA damage and repair in plants, Ann. Rev. Plant Physiol. Plant Mol. Biol., 47, 75-100 crossref(new window)

Hideg, E., J. Mano, C. Ohno and K. Asada, 1997, Increased level of monodehydro- ascorbate radical in UV-B irradiated broad bean leaves, Plant Cell Physiol., 38, 684-690 crossref(new window)

Jayakumar, M., P. Amudha and G. Kulandaivelu, 2004, Effect of low doses of UV-A and UV-B radiation on photosynthetic activities in Phaseolus mungo L., J. Plant Biol., 47, 105-110 crossref(new window)

Bornman, J. F., C. Sundby-Emanuelsson, Y. P. Cen and C. Alenius, 1994, UV-B radiation and the photosynthetic process, In Biggs R. H. and M. E. B. Joyner (eds.) Stratospheric ozone delpletion / UV-B radiation in the biosphere, Berlin, Germany, Springer-Verlag, 67-76

Olsson, L. C., M. Veit and J. F. Bornman, 1999, Epidermal transmittance and phenolic composition leaves of atrazine-tolerant and sensitive cultivars of Brassica napus grown under enhanced UV-B radiation, Physiol. Plant., 107, 259-266 crossref(new window)

Cockell, C. S. and J. Knowland, 1999, Ultraviolet radiation screening compounds, Biological Reviews of the Cambridge Philosophical Society, 74, 311-345 crossref(new window)

Day, T. A., B. W. Howells and W. J. Rice, 1994, Ultraviolet absorption and epidermal-transmittance spectra in foliage, Physiol. Plant., 92, 207-218 crossref(new window)

Adamse, P., H. E. Reed, D. T. Krizk, S. J. Britz and R. M. Mirecki, 1997, An inexpensive set up for assessing the impact of ambient solar ultraviolet radiation on seedlings, J. Nat. Res. Life Sci. Edu., 26, 139-144

Saile-Mark, M. and M. Tevini, 1997, Effect of solar UV-B radiation on growth, flowering and yield of central and southern European bush bean cultivars (Phaseolus vulgaris L.), Plant Ecol., 128, 115-125 crossref(new window)

Larkindale, J. and M. R. Knight, 2002, Protection against heat stress-induced oxidative damage in Arabidopsis involves calcium, abscisic acid, ethylene and salicylic acid, Plant Physiol., 128, 682-695 crossref(new window)

Gong, M., Y. J. Li and S. Z. Chen, 1998, Abscisic acid induced thermotolerance in maize seedlings is mediated by $Ca^{2+}$ and associated with antioxidant systems, J. Plant Physiol., 153, 488-496 crossref(new window)

Koda, Y., 1997, Possible involvement of jasmonates in various morphogenetic event, Physiol. Plant., 100, 639-646 crossref(new window)

Inskeep, W. P. and P. R. Bloom, 1985, Extinction coefficients of chlorophyll a and b in N, N-dimethylformamide and 80 % acetone, Plant Physiol., 77, 483-485 crossref(new window)

Schreiber, U., U. Schliwa and W. Bilger, 1986, Continuous recording of photochemical and nonphotochemical chlorophyll fluoroscence quenching with a new type of modulation fluorometer, Photosynth. Res., 10, 51-62 crossref(new window)

Zhao, S. J., C. C. Xu, Q. Zhou and Q. W. Meng, 1994, Improvements of method for measurement of malondialdehyde in plant tissues, Plant Physiol. Comm., 30, 207-210

Reynolds, E. S., 1963, The use of lead citrate and high pH as electron opaque stain on electron microscopy, J. Cell Biol., 17, 208-212 crossref(new window)

Mark, U. and M. Tevini, 1997, Effects of solar ultraviolet-B radiation, temperature and $CO_{2}$ on growth and physiololgy of sunflower and maize seedlings, Plant Ecology, 128, 225-234 crossref(new window)

Barsig, M. and R. Malz, 2000, Fine structure, carbohydrates and photosynthetic pigments of sugar maize leaves under UV-B radiation, Environ. Exp. Bot., 43, 121-130 crossref(new window)

Jayakumar, M., P. Amudha and G. Kulandaivelu, 2003, Changes in growth and yield of Phaseolus mungo L. induced by UV-A and UV-B enhanced radiation, J. Plant Bio., 46, 59-61 crossref(new window)

Boddi, B., A. R. Oravecz and E. Lehoczki, 1995, Effect of cadimium on organization and photoreduction of photochlorophyllide in dark-grown leaves and etioplast inner membrane preparations of wheat, Photosynthetica, 31, 411-420

Karpinski, S., G. Wingsle, B. Karpinska and J. E. Hallgren, 2001, Redox sensing of photooxidative stress and acclimatory mechanisms in plants, In Aro, E. M. and B. Andersson (eds.) Regulation of Photosynthesis (Advances in Photosynthesis and Regulation, Vol. II), Kluwer Acadmic Publishers, Dordrecht, The Netherlands, pp.469-486

van Grondelle, R., J. P. Dekker, T. Gillbro and V. Sundstrom, 1994, Energy transfer and trapping in photosynthesis, Biochim. Biophys. Acta., 1187, 1-65 crossref(new window)

Olsson, L. C., L. Fraysee and J. F. Bornmann, 2000, Influence of high light and UV-B irradiation on photosynthesis and D1 turnover in atrazine-tolerant and -sensitive cultivars of Brassica napus, J. Exp. Bot., 51, 265-274 crossref(new window)

Kristian, R. A., T. N. Mikkelsen and H. R. Poulsen, 2005, Effects of ambient versus reduced UV-B radiation on higy arctic Salix arctica assessed by measurements and calculations of chlorophyll a fluorescence parameters from fluorescence transients, Physiol. Plant., 124, 208-226 crossref(new window)

Uzunova, A. N. and L. P. Popova, 2000, Effect of salicylic acid on leaf anatomy and chloroplast ultrastructure of barley plants, Photosynthetica, 38, 243-250 crossref(new window)

Dekov, I., T. Tsonev and I. Yordanov, 2000, Effects of water stress and high-temperature stress on the structure and activity of photosynthetic apparatus of Zea mays and Helianthus annuus, Photosynthetica, 38, 361-366 crossref(new window)