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Chromosome Aberration in Suspension Culture of Ginseng(Panax ginseng C. A. Meyer) Callus
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 Title & Authors
Chromosome Aberration in Suspension Culture of Ginseng(Panax ginseng C. A. Meyer) Callus
Park, Jong-Bum;
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 Abstract
This study was to examine the variations of chromosome number and the ranges of variety in the suspension culture of ginseng (Panax ginseng C. A. Meyer) callus cell, and the effect of plant hormones for the chromosome aberration. Plant hormones added with MS medium in the suspension culture were 2,4-D, kinetin, and 2,4-D+kinetin and concentration of the plant hormones were and respectively. As a result of these experiment the following conclusion has been obtained. Media contained with 2,4-D+kinetin in concentration was very effective in the suspension culture result from 26.4% mitosis frequency, and found the various variation of chromosome number. Variety of chromosome number was diversed (), espicially frequency of hypohaploid and hyperhaploid cells were very higher than hyperdiploid cells. In this experiments, it is suggested that 2,4-D+kinetin added with medium in the suspension culture of ginseng callus was effect in the variations of chromosome number.
 Keywords
Chromosome aberration;Suspension culture;Ginseng;Callus;
 Language
Korean
 Cited by
1.
장뇌삼의 2차 체세포배 발생을 통한 식물체 유도 및 순화,이수광;김지희;강호덕;

한국임학회지, 2008. vol.97. 1, pp.127-133
 References
1.
Partanen, C. R., 1963, Plant tissue culture in relation to developmental cytology, Intern. Rev. Cytol., 15, 215-243 crossref(new window)

2.
Murashige, T. and R. Nakano, 1967, Chromosome complement as a determinant of the morphogenic potential of tobacco cells, Amer. J. Bot., 54(8), 963-970 crossref(new window)

3.
Yazawa, S., 1967, Cytological and morphological observations on the callus tissue of Crepis capillaris, Bot. Mag., 80, 413-420 crossref(new window)

4.
Kao, K. N., R. A. Miller, O. L. Gamborg and B. L. Harvey, 1970, Variations in chromosome number and structure in plant cells grown in suspension culture, Can. J. Genet. Cytol., 12, 297-301 crossref(new window)

5.
Sacristan, M. D., 1971, Karyotypic changes in callus cultures from haploid and diploid plants of Crepis capillaris Wallr., Chromosoma, 33, 273-283

6.
Torrey, J. G., 1959, Experimental modification of development in the root, Symp. Soc. Study Develop. Growth, 17, 189-222

7.
Venketeswaran, S., 1963, Tissue culture studies on Vicia faba, II. Cytology, Caryologia, 16, 91-100 crossref(new window)

8.
Mitra, J. and F. C. Steward, 1961, Growth induction in cultures of Haploppus gracilis, II. The behaviour of the nucleus, Amer. J. Bot., 48, 358-368 crossref(new window)

9.
Torrey, J. G., J. Reinert and M. Merkel, 1962, Mitosis in suspension cultures of higher plant cells in a synthetic medium, Amer. J, Bot. 49, 420-425 crossref(new window)

10.
Heinz, D. J., G. W. P. Mee and L. G. Nickell, 1969, Chromosome number of some Saccharum species hybrids and their cell suspension cultures, Amer. J. Bot., 56(4), 450-456 crossref(new window)

11.
DeTorok, D. and P. R. White, 1960, Cytological instability in tumor of Picea glaica, Cytochem., 17, 266-272

12.
Nishiyama, I. and T. Taira, 1966, The effect of kinetin and indol acetic acid on callus growth and organ formation in two species of Nicotiana, Jap. J. Gentics., 41, 357-366 crossref(new window)

13.
Singh, B. D. and B. L. Harvey, 1975, Cytogenetic studies on Haplopappus gracilis cells cultured on agar and in liquid media, Cytologia, 40, 347-354

14.
Cooper, L. S., D. C. Cooper, A. C. Hildebrandt and A. J. Riker, 1964, Chromosome numbers in single cell clones of tobacco tissue, Amer. J. Bot., 31, 284-190

15.
Shimada, T. and M. Tabata, 1967, Chromosome numbers in cultured pith tissue of tobacco, Jap. J. Genet., 42, 195-201 crossref(new window)

16.
竹中要(Y. Takenaka), 1937, 人蔘細胞の染色体に關よろ硏究, 朝鮮博物誌, 22, 53-61

17.
Lee, C. D. and S. S. Hong, 1980, Study on breeding of Korean ginseng (Panax ginseng C. A. Meyer), Sung Kyun Kwan Univesity Journal, 27, 1-11

18.
Lee, C. D. and S. S. Hong, 1978, Studies on callus cultures of medical plant in vitro, II. On the callus culture of Panax ginseng C. A. Meyer, Sung Kyun Kwan Univesity Journal Natural Science, 6, 5-11

19.
Masuda, Y., 1965, RNA in relation to the effect of auxin, kinetin and gibberellic acid on the tuber tissue of Jerusalem artichoke, Physiol. Plant., 18, 15-22 crossref(new window)

20.
Ogura, H., 1975, Morphactin-kinetin interaction on growth and shoot formation in tobacco callus cultures, Plant Physiol., 16, 563-569

21.
Torrey, J. G., 1961, Kinetin as trigger for mitosis in mature endomitotic plant cells, Exper. Cell Res., 23, 281-299 crossref(new window)

22.
Prasad, G. and K. Das, 1977, Effects of some growth substances on mitosis, Cytologia, 42, 323-329 crossref(new window)

23.
Vig, B. K., 1969, Relationship between mitotic events and leaf spotting in Glycine max, Can. J. Genet. Cytol., 11, 147-152 crossref(new window)