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Conditions for Soluble Phosphate Production by Environment-Friendly Biofertilizer Resources, Pseudomonas fluorescens
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 Title & Authors
Conditions for Soluble Phosphate Production by Environment-Friendly Biofertilizer Resources, Pseudomonas fluorescens
Park, Ki-Hyun; Park, Geun-Tae; Kim, Sung-Man; Lee, Chung-Yeol; Son, Hong-Joo;
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The effects of inorganic salts, inoculum concentration, aeration rate and shaking speed on insoluble phosphate solubilization by Pseudomonas fluorescens RAF15 were investigated. Soluble phosphate production was dependent on the presence of and in the medium. Supplementation of medium with 0.01% and 0.01% NaCl slightly increased soluble phosphate production. The optimal medium compositions for the solubilization of insoluble phosphate by P. fluorescens RAF15 were 1.5% glucose, 0.005% urea, 0.3% , 0.01% , 0.01% and 0.01% NaCl, respectively. Optimal inoculum concentration was 2.0%(v/v). Maximum soluble phosphate production was obtained with 20-50 ml/250-ml flask and 200 rpm of shaking speed, respectively. The addition of EDTA decreased cell growth and soluble phosphate production.
Biofertilizer;Phosphate-solubilizing bacteria;Pseudomonas fluorescens;
 Cited by
RAF15 isolated from ginseng rhizosphere and its plant growth-promoting activities, Letters in Applied Microbiology, 2009, 49, 2, 222  crossref(new windwow)
Vassilev N., Baca M. T., Vassileva M., Franco I., Azcon R., 1995, Rock phosphate solubilization by Aspergillus niger grown on sugar-beet waste medium, Appl. Microbiol. Biotechnol., 44, 546-549

Narsian V., Patel H. H., 2000, Aspergillus aculeatus as a rock phosphate solubilizer, Soil Biol. Biochem., 32, 559-565 crossref(new window)

Reyes I., Bernier L., Simard R. R., Antoun H., 1999, Effect of nitrogen source on the solubilization of different inorganic phosphates by an isolate of Penicillium rugulosum and two UV-induced mutants, FEMS Microbiol. Ecology 28, 182-190

Vassilev N., Vassileva M., 2003, Biotechnological solubilization of rock phosphate on media containing agro-industrial wastes, Appl. Microbiol. Biotechnol., 61, 435-440 crossref(new window)

Rodriguez H., Reynaldo F., 1999, Phosphate solubilizing bacteria and their role in plant growth promotion, Biotechnol. Adv., 17, 319-339 crossref(new window)

Illmer P., Schinner F., 1992, Solubilisation of inorganic phosphates by microorganisms isolated from forest soils, Soil Biol. Biochem., 24, 389-395 crossref(new window)

Illmer P., Schinner F., 1995, Solubilisation of inorganic calcium phosphates: solubilisation mechanisms, Soil Biol. Biochem., 27, 257-263 crossref(new window)

Park K. H., Son H. J., 2006, Isolation and characterization of insoluble phosphate-solubilizing bacteria with antifungal activity, Kor. J. Microbiol., 42, 223- 229

Rodriguez H., Gonzalez T., Selman G., 2000, Expression of a mineral phosphate solubilizing gene from Erwinia herbicola in two rhizobacterial strains, J. Biotechnol., 84, 155-161 crossref(new window)

Clesscerl L. S., Greenberg A. E., Eaton A. D., 1998, Standard methods for the examination of water and wastewater, 20th ed., APHA-AWWA-WEF, Washington, D.C

Nautiyal C. S., Bhadauria S., Kumar P., Lal H., Mondal R., Verma D., 2000, Stress induced phosphate solubilization in bacteria isolated from alkaline soils, FEMS Microbiol. Lett., 182, 291-296 crossref(new window)

Pirt S. J., 1975, Principles of microbe and cell cultivation, John Wiley & Sons, New York