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Temporal Changes in Abundances of the Toxic Dinoflagellate Alexandrium minutum (Dinophyceae) in Chinhae Bay, Korea
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 Title & Authors
Temporal Changes in Abundances of the Toxic Dinoflagellate Alexandrium minutum (Dinophyceae) in Chinhae Bay, Korea
Park, Tae-Gyu; Kang, Yang-Soon;
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Marine dinoflagellate Alexandrium minutum producing paralytic shellfish toxins is responsible for paralytic shellfish poisoning (PSP). To investigate its temporal distributions in Chinhae Bay where PSP occurs annually, SYBR Green I based A. minutum-specific real-time PCR probe was developed on the LSU rDNA region. Assay specificity and sensitivity were tested against related species, and its specificity was further confirmed by sequencing of field-derived samples. Ten months field survey in 2008 (a total 100 surface water samples) by using the real-time PCR probe showed that A. minutum was detected at very low densities of 1-4 cells in May and June being spring in Chinhae Bay, Korea.
Alexandrium minutum;Dinoflagellate;PSP toxins;Real-time PCR;
 Cited by
2013년 하계 국내 연안에서 출현하는 Alexandrium 속 와편모류의 종 다양성,김재성;박경우;윤석현;임월애;유영두;성경아;이원호;

한국해양학회지:바다, 2016. vol.21. 4, pp.158-170 crossref(new window)
Species Diversity of the Dinoflagellate Genus Alexandrium in the Coastal Waters of Korea during Summer 2013, The Sea, 2016, 21, 4, 158  crossref(new windwow)
Hallegraeff G. M., 1993, A review of harmful algal blooms and their apparent global increase, Phycologia, 32, 79-99 crossref(new window)

Hallegraeff G. M., 2003, Harmful algal blooms: a global overview. In Hallegraeff G. M., Anderson D. M., Cembella A. D. (ed.), Manual on Harmful Marine Microalgae, United Nations Educational, Scientific and Cultural Organization, Paris, 25-49

Cembella A. D., 1998, Ecophysiology and metabolism of paralytic shellfish toxins in marine microalgae, In Anderson D. M., Cembella A. D., Hallegraff G. M. (ed.), Physological Ecology of Harmful Algal Blooms, Springer Verlag, Berlin, Heidelberg, 381-404

Cho C. H., 1978, On the Gonyaulax red tide in Jinhae Bay. Bull. Korean Fish. Soc., 11, 111-114

Balech E., 1995, The genus Alexandrium Halim (Dinoflagellata), Sherkin Island Marine Station, Ireland

Bowers H. A., T. Tengs, H. B. Glasgow, J. M. Burkholder, P. A. Rublee and D. W. Oldach, 2000, Development of real-time PCR assays for rapid detection of Pfiesteria piscicida and related dinoflagellates, Appl. Environ. Microbiol., 66, 4641-4648 crossref(new window)

Zhang H. and S. Lin, 2005, Development of a cob-18S rDNA gene real-time PCR assay for quantifying Pfiesteria shumwayae in the natural environment, Appl. Environ. Microbiol., 71, 7053-7063 crossref(new window)

Park T. G., M. F. de Salas, C. J. S. Bolch and G. M. Hallegraeff, 2007, Development of a real-time PCR probe for quantification of the heterotrophic dinoflagellate Cryptoperidiniopsis brodyi (Dinophyceae) in environmental samples, Appl. Environ. Microbiol., 73, 2552-2560 crossref(new window)

Park T. G., Y. T. Park and Y. Lee, 2009a, Development of a SYTO9 based real-time PCR probe for detection and quantification of toxic dinoflagellate Karlodinium veneficum (Dinophyceae) in environmental samples, Phycologia, 48, 32-43 crossref(new window)

Park T. G., G. H. Park, Y. T. Park, Y. S. Kang, H. M. Bae, C. H. Kim, H. J. Jeong and Y. Lee, 2009b, Identification of the dinoflagellate community during Cochlodinium polykrikoides (Dinophyceae) blooms using amplified rDNA melting curve analysis and real-time PCR probes. Harmful Algae, 8, 430-440 crossref(new window)

Guillard R. R. L. and J. H. Ryther, 1962, Studies of marine planktonic diatoms. Ⅰ. Cyclotella nana Hustedt and Detonula confervacea cleve, Can. J. Microbiol., 8, 229-239 crossref(new window)

Hosoi-Tanabe S. and Y. Sako, 2005, Species-specific detection and quantification of toxic marine dinoflagellates Alexandrium tamarense and A. catenella by real-time PCR assay, Mar. Biotechnol., 7, 506-514 crossref(new window)

Thompson J. D., T. J. Gibson, F. Plewnaik, F. Jeanmougin and D. G. Higgins, 1997, The CLUSTALX windows interface: flexible strategies for multiple sequence alignment aided by quality tools, Nucleic Acids Res., 25, 4876-4882 crossref(new window)

Galluzzi L., A. Penna, E. Bertozzini, M. Vila, E. Garcés and M. Magnani, 2004, Development of a real-time PCR assay for rapid detection and quantification of Alexandrium minutum (a Dinoflagellate), Appl. Environ. Microbiol., 70, 1199-1206 crossref(new window)

Wilson I. G., 1997, Inhibition and facilitation of nucleic acid amplification, Appl. Environ. Microbiol. 63, 3741-3751

Hansen G., N. Daugbjerg and J. M. Franco, 2003., Morphology, toxin composition and LSU rDNA phylogeny of Alexandrium minutum (Dinophyceae) from Denmark, with some morphological observations on other European strains. Harmful Algae, 2, 317-335 crossref(new window)

Lin S., H. Zhang and A. Dubois, 2006, Low abundance distribution of Pfiesteria piscicida in Pacific and Western Atlantic as detected by mtDNA-18S rDNA real-time polymerase chain reaction, J. Plank. Res., 28, 667-681 crossref(new window)

NFRDI, 2008, Data on shellfish poisoning. (written in Korean)