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Isolation and Identification of a Biphenyl-degrading Bacterium, Pseudomonas sp. DS-94
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 Title & Authors
Isolation and Identification of a Biphenyl-degrading Bacterium, Pseudomonas sp. DS-94
Lee, Dae-Sung; Jeong, Seong-Yun;
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Three biphenyl-degrading microorganisms were isolated from polluted soil samples in Sasang-gu, Busan. Among them, isolate DS-94 showing the strong degrading activity was selected. The morphological, physiological, and biochemical characteristics of DS-94 were investigated by API 20NE and other tests. This bacterium was identified as the genus Pseudomonas by 16S rDNA sequencing and designated as Pseudomonas sp. DS-94. The optimum temperature and pH for the growth of Pseudomonas sp. DS-94 were and pH 7.0, respectively. This isolate could utilize biphenyl as sole source of carbon and energy. Biphenyl-degrading efficiency of this isolate was measured by HPLC analysis. As a result of biological biphenyl-degradation at high biphenyl concentration (500 mg/L), biphenyl-removal efficiency by this isolate was 73.5% for 7 days.
Biphenyl-degrading activity;Contaminated soil;Pseudomonas sp. DS-94;
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