Advanced SearchSearch Tips
Optimal Culturing and Enhancement of Lipid Accumulation in a Microalga Botryococcus braunii
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Optimal Culturing and Enhancement of Lipid Accumulation in a Microalga Botryococcus braunii
Kwon, Sung-Hyun; Lee, Eun-Mi; Cho, Dae-Chul;
  PDF(new window)
Several tests and experimental work have been done for identifying the best growth conditions and accumulated amount of lipid moiety in B. braunii, a microalga(UTEX 572) in terms of media composition. The specific growth rate was found to be the highest at 0.15 g/L-day when the phosphorus concentration was doubled with the other ingredients at the normal level. Experiments for varied media compositions revealed that the accumulation of lipid was the highest at 48% (dry cell weight based) in the nitrogen deficient medium and its corresponding specific growth rate was comparative to that in the normal BG 11 medium. In the bubble column experiments, carbon dioxide containing air produced four times more cell mass than air only. Light and glucose addition also enhanced cell mass with maximum, 1.8 g/L and accordingly 42% of lipid composition, which turned out to be a better strategy for higher lipid-producing microalgal culture.
Botryococcus braunii;Lipid content;Optimal culturing;
 Cited by
pH와 탄소원이 Chlorella sorokiniana의 heterotrophic 배양 및 하폐수고도처리능에 미치는 영향,박정은;조용범;장산;황선진;

상하수도학회지, 2013. vol.27. 6, pp.703-709 crossref(new window)
Mixotrophic 미세조류를 이용한 유기물 및 영양염류 제거에 미치는 pH 및 폭기의 영향,김선진;이윤희;황선진;

상하수도학회지, 2013. vol.27. 1, pp.69-76 crossref(new window)
무기탄소원으로서의 NaHCO3가 미세조류 Scenedesmus dimorphus의 성장에 미치는 영향 평가,주성진;장산;최경진;이석민;황선진;

상하수도학회지, 2014. vol.28. 5, pp.555-560 crossref(new window)
Effects of pH and aeration rates on removal of organic matter and nutrients using mixotrophic microalgae, Journal of Korean Society of Water and Wastewater, 2013, 27, 1, 69  crossref(new windwow)
Effects of sodium bicarbonate as an inorganic carbon source on the growth of scenedesmus dimorphus, Journal of Korean Society of Water and Wastewater, 2014, 28, 5, 555  crossref(new windwow)
오희목, 김정석, 이석준, 1998, 미세조류에 의한 지구온난화기체의 고정화, Korean J. Environ. Biol., 16(4), 291-297.

Bajpai, P., Bajpai, P. K., 1993, Eicosapentaenoic acid (EPA) production from microorganisms: a review, Journal of biotechnology, 30(2), 161-183. crossref(new window)

Belarbi, E. -H., Molina, G. E., Chisti, Y., 2006, A process for high yield and scaleable recovery of high purity eicosapentaenoic acid esters from microalgae and fish oil, Enzyme Microbiol. Technol., 26, 516-529.

Chisti, Y., 2007, Diesel from microalgae, Biotechnology Advances, 25, 294-306. crossref(new window)

Dijkstra, A. J., 2006, Revisiting the formation of trans isomers during partial hydrogenation of triacylglycerol oils, Eur. J. Lipid Sci. Technol., 108(3), 249-264. crossref(new window)

Endo, H., Hosoya, H., Koibuchi, T., 1977, Growth yields of Chlorella regularis in dark-heterotrophic continuous cultures using acetate. J. Ferment. Technol., 55, 369-370.

Grima, E. M., Camacho, F. G., Perez, J. A. S., 1994, Biochemical productivity and fatty acid profiles of Isochrysis galbana Parke and Tetraselmis sp. as a function of incident light intensity, Process Biochemistry, 29(2), 119-126. crossref(new window)

Kenyon, C. N., Rippka, R., Stanier, R. Y., 1972, Fatty acid composition and physiological properties of some filamentous blue-green algae, Archiv für Mikrobiologie, 83(3), 216-236. crossref(new window)

Kosaric, N., Velikonja, J., 1995, Liquid and gaseous fuels from biotechnology: Challenge and opportunities, FEMS Microbiol. Rev., 16, 111-142. crossref(new window)

Lee, S. J., Kim, S. B., Kim, J. E., Kwon, G. S., Yoon, B. D., Oh, H. M., 1998, Effects of harvesting time and growth stage on the flocculation of the green alga Botryococcus braunii, Lett. Appl. Microbiol., 27, 14-18. crossref(new window)

Lee, S. J., Yoon, B. D., Oh, H. M., 1998, Rapid method for the determination of lipids from the green alga Botryococcus braunii, Biotechnol. Tech., 12, 553-556. crossref(new window)

Livne, A., Sukenik, A., 1992, Lipid Synthesis and abundance of Acetyl CoA carboxylase in Isochrysis galbana(Prymnesiophyceae) following nitrogen starvation, Plant and Cell Physiology, 33(8), 1175-1181.

Lorenz, R. T., Cysewski, G. R., 2003, Commercial potential for Haematococcus microalga as a natural source of astaxanthin, Trends Biotechnol., 18, 160-167.

Metzger, P., Largeau, C., 2005, Botryococcus braunii: a rich source for hydrocarbons and related ether lipids, Appl. Microbiol. Biotechnol., 66, 486-496. crossref(new window)

Molina, E., Martinez, E., Sanchez, S., 1991, The influence of temperature and the initial N:P ratio on the growth of microalgae Tetraselmis sp., Process Biochemistry, 26(3), 183-187. crossref(new window)

Spolaore, P., Joannis-Cassan, C., Duran, E., Isambert, A., 2006, Commercial applications of microalgae, J. Biosci. Bioeng., 101, 87-96. crossref(new window)

Wolf, F. R., Nonomura, A. M., Bassham, J. A., 1985, Growth and branched hydrocarbon production in a strain of Botryococcus braunii(Chlorophyta). J. Phycol., 21, 388-396.

Wyman, C. E., Goodman, B. J., 1993, Biotechnology for production of fuels, chemicals, and materials from biomass, Appl. Biochem. Biotech., 39/40, 41-59. crossref(new window)

Xu, H., Miao, X., Wu, Q., 2006, High quality biodiesel production from a microalga Chlorella protothecoides by heterotrophic growth in fermenters. Journal of biotechnology, 126(4), 449-507.

Yongmanitchai, W., Ward, O. P., 1991, Screening of algae for potential alternative sources of eicosapentaenoic acid, Phytochemistry, 30(9), 2963-2967. crossref(new window)