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Acceleration of Biological Denitrification by Using Bioelectrochemical Reactor
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 Title & Authors
Acceleration of Biological Denitrification by Using Bioelectrochemical Reactor
Chun, Ji-Eun; Yu, Jae-Cheul; Park, Young-Hyun; Seon, Ji-Yun; Cho, Sun-Ja; Lee, Tae-Ho;
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Nitrate contamination of water environments can create serious problems such as eutrophication of rivers. Conventional biological processes for nitrate removal by heterotrophic denitrification often need additional organic substrates as carbon sources and electron donors. We tried to accelerate biological denitrification by using bioelectrochemical reactor (BER) in which electrode works as an electron donor. Denitrification activity of 8 environmental samples from various sediments, soils, groundwaters, and sludges were tested to establish an efficient enrichment culture for BER. The established enrichment culture from a soil sample showed stable denitrification activity without any nitrite accumulation. Microbial community analysis by using PCR-DGGE method revealed that dominant denitrifiers in the enrichment culture were Pantoea sp., Cronobacter sakazakii, and Castellaniella defragrans. Denitrification rate () of the enrichment culture in BER with electrode poised at -0.5 V (vs Ag/AgCl) was higher than that () of BER without any poised potential. This results suggested that biological denitrification would be improved by supplying potential throughout electrode in BER. Further research using BER without any organic substrate addition is needed to apply this system for bioremediation of water and wastewater contaminated by nitrate.
Bioelectrochemical systems;Denitrification;Enrichment;Nitrate-nitrogen;
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Blaszczyk, M., Mycielski, T., Jaworowska-Deptuch, H., Grzostek, K., 1980, Effect of various sources of organic carbon and high nitrite and nitrate concentrations on the selection of denitrifying bactera. I. Stationary cultures, Acta Microbiol. Pol., 29(4), 397-406.

Clauwaert, P., Rabaey, K., Aelterman, P., Schamphelaire, L. D, Pham, T. H., Boeckx, P., Boon, N., Verstraete, W., 2007, Biological denitrification in microbial fuel cells, Envion. Sci. Technol., 41(9), 3354-3360. crossref(new window)

Dermont, C. B., Mary, K. W., Rao, Y. S., 1992, Nitrate contamination of groundwater: Sources and potential health effects, J. AWWA, 84(9), 85-90.

EPA, US., 2011, National Primary Drinking Water Standards, EPA,

Ergas, S. J., Reuss, A. F., 2001, Hydrogenotrophic denitrification of drinking water using a hollow fibre membrane bioreactor, J. Water Supply: Res. Technol., 50(3), 161-171.

Feleke, Z., Sakakibara, Y., 2002, A bio-electrochemical reactor coupled with adsorber for the removal of nitrate and inhibitory pesticide, Water Research, 36(12), 3092-3102. crossref(new window)

Gregory, K. B., Bond, K. R., Lovley, D. R., 2004, Graphite electrodes as electron donors for anaerobic respiration, Env. Microbiology, 6(6), 596-604. crossref(new window)

Islam, S., Suidan, M. T., 1998, Electrolytic denitrification: Long term performance and effect of current intensity, Water Res., 32(2),528-536. crossref(new window)

Liu, Q. M., Ten, L. N., Im, W. T., Lee, S. T., 2008, Castellaniella caeni sp. nov., a denitrifying bacterium isolated from sludge of a leachate treatment plant, IJSEM, 58(9), 2141-2146.

Meinhold, J., Arnold, E., Isaacs, S., 1999, Effect of nitrite on anoxic phosphate uptake in biological phosphorus removal activated sludge, Water Research, 33(8), 1871-1883. crossref(new window)

Muyzer, G., Waal, E. C., Uitterlinden, A. G., 1993, Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding 16S rRNA, Am. Soc. Microbiol., 59(3), 695-700.

Nishimura, R., Kashishian, W. L. A., Mondino, A., Zhou, M., Cooper, J., Schlessinger, J., 1993, Two signaling molecules share a phosphotyrosine containing binding site in the PDGF receptor, Am. Soc. Microbiol., 13(11), 6889-6896.

Park, H. I., Kim, D., Choi, Y. J., Pak, D., 2005, Nitrate reduction using an electrode as direct electron donor in a biofilm-electrde reactor, Process Biochemistry, 40(10), 3383-3388. crossref(new window)

Park, S., Yu, J., Byun, I., Cho, S., Park, T., Lee, T., 2011, Microbail community structure and dynamics in a mixotrophic nitrigen removal process using recycled spent caustic under different loading conditions, Bioresource Technol., 102(15), 7265-7271. crossref(new window)

Prosnansky, M., Sakakibara, Y., 2002, High-rate denitrification and SS rejection by biofilm-electrode reactor(BER) conbined with microfiltration, Water Res., 36(19), 4801-4810. crossref(new window)

Rijin, J. V., Tal, Y., Schreier, H. J., 2006, Denitrification in recirculating systems: theory and applications, Aquacul. Eng., 34(3), 364-376. crossref(new window)

Shrimali, M., Singh, K. P., 2001, New methodes of nitrate removal from water, Environ. Pollut. 112(3), 351-359. crossref(new window)

Weisburg, W. G., Barns, S. M., Pelletier, D. A., Lane, D. J., Bacteriol, J., 1991, 16S ribosomal DNA amplification for phylogenetic study, Am. Soc. Microbiol., 173(2), 697-703.

Wilderer, P. A., Jones, W. L., Dau, U., 1987, Competition in denitrification systems affecting reduction rate and accumulation of nitrite, Water Res., 21(2), 239-245. crossref(new window)

Yu, J., Kim, D., Lee, T., Microbial diversity in biofilm on water distribution pipes of different materials, Water Science Technology, 61, 163-171(2010). crossref(new window)

Yu, J., Cho, S., Kim, S., Cho, H., Lee, T., Comparison of exoelectrogenic bacteria detected by using two different methods: U-tube microbial fuel cell and plating method, Microbes and Environments, 27(1), 49-53(2012). crossref(new window)

Zhang, Y., Min, B., Huang, L., Angelidaki, I., 2011, Electricity generation and microbial community response to substrate changes in microbial fuel cell, Bioresource Technology, 102(2), 1166-1173. crossref(new window)