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The Electrophysiology Application on Guard Cells to See the Influence of Carbon Dioxide
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 Title & Authors
The Electrophysiology Application on Guard Cells to See the Influence of Carbon Dioxide
Lee, Joon-Sang;
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The effect of on the opening of stomata in the intact leaf of Commelina communis has been investigated. Full opening of stomatal apertures(around ) was achieved in the intact leaf by addition of (). At 90 minutes, the stomatal apertures of leaves treated with free air were reduced. In contrast, stomata opened most widely with the treatment of air at 90 minutes. The effects of light, air and free air on the change of membrane potential difference(PD) were measured. Fast hyperpolarization of guard cell membrane PD was recorded reaching up to -12 mV in response to light. If free air was given firstly, there was no response. When light was given after free air, the light effect was very clear. At the onset of air, the PD showed a dramatic hyperpolarization to about -25 mV. Changes in the pH of apoplast in intact leaves in response to air were observed. air caused a change of 0.4 pH unit. Therefore, it can be hypothesized that flowing could stimulate proton efflux which is a necessary precursor of stomatal opening.
;Commelina communis;Stomatal aperture;Membrane potential difference;
 Cited by
Bowing, D. J. F., 1989, Intracellular and intercellular pH measurement with microelectrodes, Methods Enzym., 174, 331-338. crossref(new window)

Edwards, M. C., Smith, G. N., Bowing, D. J. F., 1988, Guard cells extrude protons prior to stomatal opening, J Exp. Bot., 39, 1541-1547. crossref(new window)

Farquhar, G. D., Dubbe, D. R., Raschke, K., 1978, Gain of the feedback loop involving carbon dioxide and stomata, Plant Physiol., 62, 406-412. crossref(new window)

Gotow, K., Taylor, S., Zeiger, E., 1988, Photosynthetic carbon fixation in guard cell protoplasts of Vicia faba L, Plant Physiol., 86, 700-705. crossref(new window)

Higinbotham, N., 1973, Electropotentials of plant cells, Ann. Rev. Plant Physiol., 24, 25-46. crossref(new window)

Hsiao, T. C., Allaway, W. G., Evans, L. T., 1973, Action spectra for guard cell $Rb^{2+}$ uptake and stomatal opening in Vicia faba, Plant Physiol., 51, 82-88. crossref(new window)

Jarvis, P. G., Morison, J. I. L., 1981, The control of transpiration and photosynthesis by the stomata, in: P. G. Jarvis, and Mansfield, T. A. (eds,), Stomatal physiology, Cambridge University Press, 247-279.

Kim, C. Y., 2012, Stomatal responses of C3 and C4 cyperus species(Cyperaceae) in Korea to elevated $CO_2$ concentration, M.S.D. Dessetation, Sungshin Women's University, Seoul, Korea.

Kim, D. J., Lee, J. S., 2007, Current theories for mechanism of stomatal opening, J Plant Biol., 50, 523-526. crossref(new window)

Kuiper, P. J. C., 1964, Dependence upon wavelength of stomatal movement in epidermal tissue of Senecio odoris, Plant Physiol., 39, 952-955. crossref(new window)

Lee, J. S., Bowling, D. J. F., 1995, Influence of the mesophyll on stomatal opening, Aust. J Plant Physiol., 22, 357-363. crossref(new window)

Lee, J. S., 2010. Stomatal opening mechanism of CAM plants, J Plant Biol., 53, 19-23. crossref(new window)

Lee, J. S., 2013. Do really close stomata by soil drying ABA produced in the roots and transported in transpiration stream? American J Plant Science, 4: 169-173. crossref(new window)

Lee, J. S., Bowling, D. J. F., 1992, Effect of the mesophyll on stomatal opening in Commelina communis, J Exp. Bot., 43, 951-957. crossref(new window)

Lee, J. S., Bowling, D. J. F., 1993, Influence of the mesophyll on the change of electrical potential difference of guard cells induced by red light and $CO_2$ in Commelina communis L. and Tradescantina virginiana L, Kor. J. Bio., 36, 383-389.

Leymarie, J., Vavasseur, A., Lsaceve, G., 1999, $CO_2$ sensingin stomata of abi1-1 and abi2-1 mutants of Arabidopsis thaliana, Plant Physiol. Biochem, 36, 539-543.

Melis, A., Zeiger, E., 1982, Modulation of guard cell photophsphorylation by $CO_2$, Plant Physiol., 69, 642-647. crossref(new window)

Meidner, H., Mansfield, T. A., 1965, Stomatal response to illumination, Biological Review, 40, 483-509. crossref(new window)

Morison, J. I. L., 1987, Intercellular concentration and stomatal response to $CO_2$, in: Zeiger, E., Farquhar, G.D., Cowan, I. R., (eds.), Stomatal function, Stanford, California, USA, 229-251.

Ogawa, T., Ishikawa, K., Shimada, K., Shibata, K., 1978, Synergistic action of red and blue light and action spectra for malate formation in guard cells of Vicia faba L., Planta, 142, 61-65. crossref(new window)

Pallaghy, C. K., 1968, Electrophysiological studies in guard cells of tobacco. Plant Physiol., 64, 79-82.

Ramos, C., Hall, A. E., 1983, Effects of photofluorescence rate and intercellular partial pressure on leaf conductance and $CO_2$ uptake rate in Capsicum and Amaranthus, Photosynthetica, 17, 34-42.

Raschke, K., 1976. How stomata resolve the dilemma of opposing priorities. Philosophical Transactions of the Royal Society, ser, B, 273, 551-560. crossref(new window)

Raschke, K., Humble, G. D., 1973, No uptake of ions required by opening stomata of Vicia faba, Planta, 115, 47-57. crossref(new window)

Shaish, A., Roth-Bejerano, N., Itai, C., 1989, The response of stomata to relate to its effect on respiration and ATP level, Physiol. Plantarum, 76, 107-111. crossref(new window)

Taiz, L., Zeiger, E., 2007, Plant Physiology. 4th ed, Sunderland: Sinauet Associates.

Weyers, J. D. B., Travis, A. J., 1981, Selection and preparation of leaf epidermis for experiments on stomatal physiology, J Exp. Bot., 32, 837-850. crossref(new window)

Wong, S. C., Cowan, I. R., Farquhar, G. D., 1978, Leaf conductance in relation to assimilation in Eucalyptus pauciflora Sieb. ex Spreng, Plant Physiol., 62, 670-674. crossref(new window)

Zeiger, E., 1986, The photobiology of stomatal movements. in: Kendrick, R. E. and Krononberg, G. H. M., (eds,), Photomorphogenesis in plants, Martinus Nijhorff Publishers, Dordrecht, Netherlands, 391-413.

Zeiger, E., 1983, The biology of stomatal guard cells. Ann. Rev. Plant Physiol., 34, 441-475. crossref(new window)