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Copper and Zinc Uptake Capacity of a Sorghum-Sudangrass Hybrid Selected for in situ Phytoremediation of Soils Polluted by Heavy Metals
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 Title & Authors
Copper and Zinc Uptake Capacity of a Sorghum-Sudangrass Hybrid Selected for in situ Phytoremediation of Soils Polluted by Heavy Metals
Oh, Soon-Ja; Koh, Seok-Chan;
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As essential trace elements, copper and zinc play important roles in many physiological events in plants. In excess, however, these elements can limit plant growth. This study selected a heavy metal-tolerant plant by analyzing seed germination and biomass of alfalfa (Medicago sativa), canola (Brassica campestris subsp. napus var. nippo-oleifera), Chinese corn (Setaria italica), and a sorghum-sudangrass hybrid (Sorghum bicolor S. sudanense), and determined heavy metal uptake capacity by analyzing biomass, chlorophyll a fluorescence, and heavy metal contents under high external copper or zinc levels. The seed germination rate and biomass of the sorghum-sudangrass hybrid were higher under copper or zinc stress compared to the other three plants. The plant biomass and photosynthetic pigment contents of the sorghum-sudangrass hybrid seedlings were less vulnerable under low levels of heavy metals ( copper or zinc). The maximum quantum yield of PSII () and the maximum primary yield of PSII () decreased with increasing copper or zinc levels. Under high copper levels, the decline in was caused only by the decline in , and was accompanied by an increase in non-photochemical quenching (NPQ). The declined under high levels of zinc due to both a decrease in the maximum fluorescence () and an increase in the initial fluorescence (), and this was accompanied by a marked decrease in photochemical quenching (qP), but not by an increase in NPQ. Accumulations of copper and zinc were found in both aboveand below-ground parts of plants, but were greater in the below-ground parts. The uptake capacity of the sorghum-sudangrass hybrid for copper and zinc reached 4459.1 mg/kg under 400 ppm copper and 9028.5 mg/kg under 1600 ppm zinc. Our results indicate that the sorghum-sudangrass hybrid contributes to the in situ phytoremediation of copper or zinc polluted soils due to its high biomass yield.
Biomass;Chlorophyll a fluorescence;Copper;Photosynthetic pigments;Seed germination;Sorghum-sudangrass hybrid;Zinc;
 Cited by
Arnon, D., 1949, Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris, Plant Physiol., 24, 1-15. crossref(new window)

Baker, A. J. M., Brooks, R. R., 1989, Terrestrial higher plants which hyperaccumulate metallic elements-a review of their distribution, ecology and phytochemistry, Biorecovery, 1, 81-126.

Clark, R. B., Pier, P. A., Knudsen, D., Maranville, J. W., 1981, Effect of trace element deficiencies and excesses on mineral nutrients in sorghum, J. Plant Nutri., 3, 357-374. crossref(new window)

Daniels, R. R., Stuckmeyer, B. E., Peterson, L. A., 1972, Copper toxicity in Phaseolus vulgaris L. as influenced by iron nutrition. I. An anatomical study, J. Amer. Soc. Hort., 9, 249-254.

Jung, K. C., Kim, B. J., Han, S. G., 1993, Survey on heavy metals content in native plant near old zinc - mining sites, Kor. J. Environ. Agric., 12, 105-111.

Kahle, H., 1993, Response of roots of trees to heavy metals, Environ. Exp. Bot., 33, 99-119. crossref(new window)

Kelly, J. M., Parker, G. R., McFee, W. W., 1979, Heavy metal accumulation and growth of seedlings of five forest species as influcnced by soil cadmium level, J. Environ. Qual., 8, 361-364.

Kriedemann, P. E., Graham, R. D., Wiskich, J. T., 1985, Photosynthetic dysfunction and in vivo changes in chlorophyll a fluorescence from manganese-deficient wheat leaves, Aust. J. Agric. Res., 36, 157-169. crossref(new window)

Kumar, P. N., Dushenkov, V., Motto, H., Raskin, I., 1995, Phytoextraction: the use of plants to remove heavy metals from soils, Environ. Sci. Technol., 29, 1232- 1238. crossref(new window)

L'Herroux, L., Le Roux, S., Appriou, P., Martinez, J., 1997, Behaviour of metals following intensive pig slurry applications to a natural field treatment process in Brittany (France)., Environ. Pollu., 97, 119-130. crossref(new window)

Lee, S. G., Kang, B. H., 2001, Feasibility of tabacco (Nicitiana tabacum L.) for phytoremediation of soil contaminated with Zn and Cu, Kor. J. Environ. Agric., 20, 211-217.

Legros, S., Doelsch, E., Feder, F., Moussard, G., Sansoulet, J., Gaudet, J. P., Bottero, J. Y., 2013, Fate and behaviour of Cu and Zn from pig slurry spreading in a tropical watersoilplant system, Agri. Ecos. Environ., 164, 70-79. crossref(new window)

Lichtenthaler, H. K., Wellburn, A. R., 1983, Determi-nations of total carotenoids and chlorophyll a and b of leaf extracts in different solvents, Biochem. Soc. Trans., 603, 591-592.

Maksymiec, W., Wjcik, M., Krupa, Z., 2007, Variation in oxidative stress and photochemical activity in Arabidopsis thaliana leaves subjected to cadmium and excess copper in the presence or absence of jasmonate and ascorbate, Chemosphere, 66, 421-427. crossref(new window)

Mallick, N., Mohn, F. H., 2003, Use of chlorophyll fluorescence in metal-stress research: a case study with the green microalga Scenedesmus, Ecotox. Environ. Saf., 55, 64-69. crossref(new window)

Martnez, C. E., Motto, H. L., 2000, Solubility of lead, zinc and copper added to mineral soils, Environ. Pollution, 107, 153-158. crossref(new window)

Maxwell, K., Johnson, G. N., 2000, Chlorophyll fluorescence-a practical guide, J. Exp. Bot., 51, 659- 668. crossref(new window)

Ministry of Environment (ME), 2014, Soil Environment Conservation Low, The Korean warning standard for agricultural land, Korean Ministry of Environment, Seoul, Korea.

Mousavi, S. R., Galavi, M., Rezaei, M., 2013, Zinc (Zn) importance for crop production, Intl. J. Agron. Plant Prod., 4, 64-68.

National Institute of Agricultural Science and Technology (NIAST), 2000, Analytical methods of soil and plant, NIAST, Rural Development Administration, Sammi Publishing Co., Suwon, Korea.

Nriagu, J. O., Panyna, J. M., 1988, Quantitative assess-ment of worldwide contamination of air, water and soils by trace metals, Nature, 333, 134-139. crossref(new window)

Ouzounidou, G., 1995, Cu-ions mediated changes in growth, chlorophyll and other ion contents in a Cutolerant Koeleria splendens, Biologia plantarum, 37, 71-78. crossref(new window)

Penha, H. G. V., Menezes, J. F. S., Silva, C. A., Lopes, G., de Andrade Carvalho, C., Ramos, S. J., Guilherme, L. R. G., 2015, Nutrient accumulation and availability and crop yields following long-term application of pig slurry in a Brazilian Cerrado soil, Nutri. Cycling Agroeco., 101, 259-269. crossref(new window)

Strasser, B. J., Strasser, R. J., 1995, Measuring fast fluorescence transients to address environmental questions: The JIP test, in: Mathis, P. (ed.), Photosynthesis: From Light to Biosphere, Kluwer Academic, Dordrecht, 977-980.

Thounaojam, T. C., Panda, P., Mazumdar, P., Kumar, D., Sharma, G. D., Sahoo, L., Panda, S. K., 2012, Excess copper induced oxidative stress and response of antioxidants in rice, Plant Physiol. Biochem., 53, 33-39. crossref(new window)