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Improvement of Virus Productivity by Sodium Butyrate in the Production of Porcine Transmissible Gastroenteritis Virus Vaccine
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  • Journal title : KSBB Journal
  • Volume 26, Issue 2,  2011, pp.107-111
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.2.107
 Title & Authors
Improvement of Virus Productivity by Sodium Butyrate in the Production of Porcine Transmissible Gastroenteritis Virus Vaccine
Lee, Chang-Jin; Kim, Cheol-Min; Jeong, Yeon-Ho;
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 Abstract
The essential operating parameters in virus vaccine production are multiplicity of infection (MOI), harvest time, and infection time. Stimulating agents also can be applied in order to improve vaccine productivity further. We investigated the optimum operating conditions in porcine transmissible gastroenteritis virus (TGEV) vaccine production and the applicability of sodium butyrate (NaBu) as a stimulating agents for the improvement of vaccine productivity. The optimum MOI, infection time, and harvest time for high production of TGEV by swine testicle (ST) cells were found to be 0.0001 pfu/cell, 3 day after cell inoculation, and 24 hpi, respectively. NaBu is known as a histone deacetylase inhibitor that has been widely used for the high expression of recombinant protein using mammalian cells and for the enhancement of virus propagation. So we tried to examine the potential of NaBu as a stimulating agent and to determine the optimum concentration by comparing TGEV titers with different range of NaBu concentration. TGEV titer with 5 mM NaBu was 1.5 times higher than control. Therefore, we concluded that NaBu can be a promising agent for stimulating various vaccine production including TGEV and the optimum NaBu concentration for TGEV production was determined to be 5 mM.
 Keywords
Porcine transmissible gastroenteritis virus;Vaccine;ST cell;Sodium butyrate;Multiplicity of infection;
 Language
Korean
 Cited by
 References
1.
Jang, J. and I. H. Kim (2010) Current status and perspectives of cell culture-based vaccine production. Kor. J. Microbial. Biotechnol. 38: 124-128.

2.
Allen, M. J., J. P. Boyce, M. T. Trentalange, D. L. Treiber, B. Rasmussen, B. Tillotson, R. Davis, and P. Reddy (2008) Identification of novel small molecule enhancers of protein production by cultured mammalian cells. Biotechnol. Bioeng. 100: 1193-1204. crossref(new window)

3.
Kruh, J. (1982) Effects of sodium butyrate, a new pharmacological agent, on cells in culture. Mol. Cell. Biochem. 42: 65-82.

4.
Chang, K. H., K. S. Kim, and J. H. Kim (1999) N-acetylcysteine increases the biosynthesis of recombinant EPO in apoptotic Chinese hamster ovary cells. Free Rad. Res. 30: 85-91. crossref(new window)

5.
Palermo, D. P., M. E. DeGraaf, K. R. Marotti, E. Rehberg, and L. E. Post (1991) Production of analytical quantities of recombinant proteins in Chinese hamster ovary cells using sodium butyrate to elevate gene expression. J. Biotechnol. 19: 35-47. crossref(new window)

6.
Kim, N. S. and G. M. Lee (2001) Overexpression of bcl-2 inhibits sodium butyrate-induced apoptosis in Chinese hamster ovary cells resulting in enhanced humanized antibody production. Biotechnol. Bioeng. 71: 184-193.

7.
Kim, N. S. and G. M. Lee (2002) Inhibition of sodium butyrateinduced apoptosis in recombinant Chinese hamster ovary cells by constitutively expressing antisense RNA of Caspase-3. Biotechnol. Bioeng. 78: 217-228. crossref(new window)

8.
Laubach, V. E., E. P. Garvey, and P. A. Sherman (1996) High-level expression of human inducible nitric oxide synthase in Chinese hamster ovary cells and characterization of the purified enzyme. Biochem. Biophy. Res. Commun. 218: 802-807. crossref(new window)

9.
Tsao, Y. S., R. Condon, E. Schaefer, P. Lio, and Z. Liu (2001) Development and improvement of a serum-free suspension process for the production of recombinant adenoviral vectors using HEK293 cells. Cytotechnology 37: 189-198. crossref(new window)

10.
Brorson, K., C. de Wit, E. Hamilton, M. Mustafa, P. G. Swann, R. Kiss, R. Taticek, G. Polastri, K. E. Stein, and X. Yuan (2002) Impact of cell culture process changes on endogenous retrovirus expression. Biotechnol. Bioeng. 80: 257-267. crossref(new window)

11.
Scholtissek, E. and K. Muller (1988) Effect of dimethylsulfoxide (DMSO) on virus replication and maturation. Arch. Virol. 100: 27-35. crossref(new window)

12.
Boriskin, Y. S., L. L. Steinberg, L. V. Dorofeeva, I. N. Zasorina, and E. P. Barkova (1988) Salt-induced enhancement of measles virus yields in cultured cells. Arch. Virol. 101: 131-136. crossref(new window)

13.
Pando, V., P. Isa, C. F. Arias, and S. Lopez (2002) Influence of calcium on the early steps of rotavirus infection. Virolog. 295: 190-200. crossref(new window)

14.
Fenner, F. J., E. P. J. Gibbs, F. A. Murphy, R. Rott, M. J. Syuddert, and D. O. White (1993) Veterinary Virology. 2nd ed., pp. 457-469. Academic Press, Inc., San Diego, CA, USA.

15.
Saif, L. J. and K. Sestak (2006) Transmissible gastroenteritis and porcine respiratory coronavirus. pp. 489-516. In: B. E. Straw, J. J. Zimmerman, S. D'Allaire, and D. J. Taylor (eds.). Diseases of Swine. 9th ed., Blackwell Publishing, Iowa, USA.

16.
Nguyen, B., K. Jarnagin, S. Williams, H. Chan, and J. Barnett (1993) Fed-batch culture of insect cells: a method to increase the yield of recombinant human nerve growth factor (rhNGF) in the baculovirus expression system. J. Biotechnol. 31: 205-217. crossref(new window)

17.
Radsak, K., R. Fuhrmann, R. P. Frank, D. Schneider, A. Kollert, K. H. Brucher, and D. Drenckhahn (1989) Induction by sodium butyrate of cytomegalovirus replication in human endothelial cells. Arch. Virol. 107: 151-158. crossref(new window)