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Comparison of 5 Assays for Quantification of Antibody to Hepatitis B Virus Surface Antigen with Immunoglobulin G Preparations
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  • Journal title : KSBB Journal
  • Volume 26, Issue 2,  2011, pp.157-164
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.2.157
 Title & Authors
Comparison of 5 Assays for Quantification of Antibody to Hepatitis B Virus Surface Antigen with Immunoglobulin G Preparations
Shin, In-Soo; Lee, Yoo-Kyoung; Kim, Oh-Jung; Ban, Sang-Ja;
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 Abstract
Five assays for anti-HBs were compared to improve potency test of Human lgG preparations. The three commercial EIA kits were optimized including dose response curve ranges and compared by conducting a co-laboratory study. After selecting the most reproducible EIA kit, methods comparison was performed with 22 samples in 5 different days. As a result, EIA (7.7 5.3%) and MEIA (AxSYM: 3.7 1.9%, IMx: 1.6 0.8%) showed precision and accuracy (100.1 12.6%). Therefore, the validated EIA assay was established and it is believed to be comparable to current MEIA.
 Keywords
Anti-HBs;Immunoglobulin preparation;Potency;National Lot-Release Test;
 Language
Korean
 Cited by
 References
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