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Screening of Functional Materials from Solvent Fractions of Apple Flower Leaf Extract
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  • Journal title : KSBB Journal
  • Volume 26, Issue 2,  2011, pp.165-171
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.2.165
 Title & Authors
Screening of Functional Materials from Solvent Fractions of Apple Flower Leaf Extract
Choi, Sun-Ju; Cho, Eun-Ah; Cho, Eun-Hye; Jeong, Yoon-Joo; Ku, Chang-Sub; Ha, Byung-Jhip; Chae, Hee-Jeong;
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Fractional solvent extraction by organic solvents such as hexane, chloroform, ethylacetate, and butanol was carried out using 70% ethanol extract of apple flower leaves. Biological activities including antioxidant, whitening, antimicrobial and anti-wrinkle activities were investigated and bio-active materials of the extracts were identified using GC/MSD. Among the tested solvent fractions, ethylacetate fraction showed the highest total polyphenol content (1218.94 ), and flavonoid (140 ). The DPPH radical scavenging activities was over 80% at a dry matterbased concentration of 200 and SOD-like activity was over 90% at 50 concentration in ethylacetate fraction that was slightly lower than of ascorbic aicd. Tyrosinase inhibition activity related to skin-whitening was over 60% by ethylacetate fraction of 100 . As an anti-aging effect, elastase inhibitory activity was about 45% in ethylacetate fraction. Also, it showed a significantly antimicrobial activity against P. acenes. From GC/MSD analysis, a characteristic peak of high content in ethylacetate fraction was identified as kaempferol, which has been reported as a bioactive compound.
apple flower leaf;kaempferol;polyphenol;solvent fraction;antioxidant;
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Fantone, J. C. and P. A Ward (1982) Role of oxygen-derived free radicals and metabolites in leukocyte dependent inflammatory reaction. Ann. J. Path. 107: 395-418.

Davies, K. J. (1987) Protein damage and degradation by oxygen radical. J. Biol. Chem. 262: 9895-9901.

Foote, C. S. (1976) Photosensitized oxidation and singlet oxygen: consequences in biological systems. Ed. W. A. Pryor. 2, 85, Acdemic Press, New York.

Temple, N. J. (2000) Antioxidants and diseases: more question than answers. Nutr. Research. 20: 449-459. crossref(new window)

Lee, S. E., D. W. Son, Y. P., Yoon, S. Y., Lee, B. J. Lee, and S. H. Lee (2006) Protective effects of the methanol extracts of Acanthopanax koreanum against oxidative stress. Kor. J. Pharm. 37: 16-20.

Nordmann, R. (1993) Free radicals, oxidative stress and antioxidant vitamins. C. R. Seances Soc. Biol. Fil. 187: 277-285.

Kenkt, P., Kumpulainen, J., Jarvinen, R., Rissanen, H., Heliovaara, M., Reunanen, A., Hakulinen, T and A. Aromaa (2002) Flavonoid intake and risk of chronic diseases. Am. J. Clin. Nutr. 76: 560-568. crossref(new window)

Cha, J. Y. and Y. S. Cho (1999) Effect of potato polyphenolics on lipid peroxidation in rats. J. Kor. Soc. Food Sci. Nutr. 28: 1131-1136.

Zhoh, C. K. and T. I. Kim (2002) The extracting methods of the seeds of yew (tsxus cusoidata sieb) by solvent extraction and its efficacy. Kor. J. 33: 364-371.

Thaycr, D. W., G. Boyd, A. Kim, J. B. Fox, and H. M. Farrcll (1998) Fate of gamma-irradiated Listeria monocytogenes during refregerated storage on rqw of cooked turkey breast meat. J. Food Prot. 61: 979-987. crossref(new window)

Cho, E. J., T. Yokozawa, D. Y. Rhyu, S. C. Kim, N. Shibahara, and J. C. Park (2003) Study on the inhibitory effects of Korean medicinal plants and their main compounds in the 1,1-diphenyl- 2-picrylhydrazyl radical. Phyromedicin. 10: 544-551. crossref(new window)

Oku, H., Y. Ueda, and K. Ishifruo (2003) Anti-pruritic effects of the fruits of chaenomeles sinensis. Biol. Pharm. Bull. 25: 1031-1034.

Gao, H., L. Wu, M. Kuroyanagi, K. Harada, N. Kawahar, T. Nakane, K. Umehara, A. Kirasawa, and Y. Nakamura (2003) Antitumor-promoting constituents from Chaenomeles sinensis K. and their acrivities in B16 mouse epidermal cells. Chem. Pharm. Bull. 51: 1318-1321. crossref(new window)

Kim, Y. M., J. Yun, C. K. Lee, H. Lee, K. R. Min, and Y. Kim (2002) Oxyresveratrol and hydroxystibene compounds, inhibitory effect on tyrosinase and mechanisim of action. J. Biol. Chem. 277: 16340-16344. crossref(new window)

Abassi, N. A., M. N. Kushad, and A. G. Endress (1998) Active oxygen scavenging enzyme activities in developing apple flowers and fruits. Sci. Hort. 74: 183-194. crossref(new window)

Park, S. Y. (2008) Protective effects of kaempferol and quercetin on oxidative stresee in CPAE cell. Kor. J. Plant Res. 24: 395-401.

Cho, C. H., G. J. Yoo, M. H. Son, K. H. Park, B. L. Lim, D. C. Kim, and H. J. Chae (2008) Resveratrol extraction from grape fruit stem and its antioxidant activity. J. Korean Soc. Appl. Biol. Chem. 51: 11-16.

Nivea, M. M., A. R. Sampoetr, and M. A. Vattuone (2000) Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. J. Ethnopharmacol. 71: 109-114. crossref(new window)

Jeong, M. S., G. S. Lee, and H. J. Chae (2004) In vitro biological activity assay of ethanol extract of radish. J. Kor. Soc. 47: 67-71.

Lee, S. O., H. J. Lee, M. H. Yu, H. K. Im, and I. S. Lee (2005) Total polyphenol contents and antioxidant activities of methanol extracts from vegetables produced in Ullung Island. J. Kor. Food Sci. Technol. 37: 233-240.

Labuza, T. P. (1973) Kinetic of lipid oxidation in foods. CRS Crit. Rev. Food Technol. p. 335.

Boo, H. O., H. H. Lee, J. W. Lee, S. J. Hwang, and S. U. Park (2009) Different of total phenolics and flavonoids, radical scavenging activities and nitrite scavenging effects of Momordica charantia L. according to cultivars. Korean J. Med. Crop Sci. 17: 15-20.

Chung, M. S., H. J. Um, C. K. Kim, and G. H. Kim (2007) Development of functional tea product using Cirsium japonicum. Kor. J. Food Culture 22: 261-265.

Park, J. M. J. Y. Lee, T. S. Park, S. J. Hyun, H. H. Kim, Y. J. Cho, O. J. Kwon, A. R. Son, D. S. Kim, and B. J. An (2008) A study on the cosmeceutical activities of Prunus sargentii R. J. Kor. Soc. Appl. Biol. Chem. 51: 70-78.

Lee, C. E., Y. H. Kim, B. G. Lee, and D. H. Lee (2010) Study on anti-oxidant effect of extracts from Pyrous ussuriensis leaves. J. Kor. For. Soc. 4: 546-552.

Kwon, T. D., S. W. Choi, S. J. Lee, K. W. Chung, and S. C. Lee (2001) Effects of polyphenol or vitamin C ingestion on antioxidative activity during exercise in rats. Kor. J. Phys. Edu. 3: 891-899.

Sung, K. C. and K. J. Kim (2005) Tyrosinase activated inhibition effect and analysis of pine-needles extract. Kor. Oil Chem. Soc. 22: 71-76.

Curelier, M. E., Richahae, H, and C. Breset (1996) Antioxidative activity of phenolic composition of pilot plant and commercial extracts of sage and rosemary. J. Am. Oil. Chem. Soc. 73: 645-652. crossref(new window)

Ra, K. S., H. J. Suh, S. H. Chung, and J. Y. Son (1997) Antioxidant activity of solvent extract from onion skin. Kor. J. Food Sci. Technol. 29: 595-6001.

Lee, S. Y., J. H. An, and H. Y. Cho (2003) Isolation and characterization of MMP-1 inhibitor peptide from crataegus pinnatifida bunge in fibroblast cell line HS68 cells. J. Kir. Soc. Agric. Chem. Biotechono. 46: 60-65.

Kwak, Y. J., D. H. Lee, N. M. Kim, and J. S. Lee (2005) Screening and extraction condition of anti-skin aging elastase ingibitior from medicinal plants. Kor. J. Medicind Crop. Sic. 13: 213-216.

Park, C. S. (2005) Component and quality characteristics of powdered green tea cultivated in Hwagae area. Kor. J. Food Preserv. 12: 36-42.

Kajiya, K., M. Ichiba, M. Kuwabara, S. Kumazawa, and T. Nakayama (2001) Role of lipophilicity and hydrogen peroxide formation in the cytotoxicity of flavonols. Biosci. Biotechnol. Biochem. 65: 1227-1229. crossref(new window)

Nakayama, T., M. Tamada, T. Osawa, and S. Kawakishi (1993) Pression of active oxygen-induced cytotoxicity by flavonoids. Biochem. Prarmacol. 45: 265-267. crossref(new window)

Kaneko, T. and N. Baba (1999) Protective effect of flavonoids on endothelial cells against linoleic acid hydroperoxideinduced toxicity. Biosci. Biochnol. Biochem. 63: 323-328. crossref(new window)