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Establishment of In vitro Detection System for iNOS Expression and the Verification of Suppressive Effect by Pine Needle Extract
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  • Journal title : KSBB Journal
  • Volume 26, Issue 2,  2011, pp.172-176
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.2.172
 Title & Authors
Establishment of In vitro Detection System for iNOS Expression and the Verification of Suppressive Effect by Pine Needle Extract
Kim, Nam-Young; Jang, Hye-Ji; Lee, Dong-Geun; Jang, Min-Kyung; Lee, Seung-Woo; Jeon, Myong-Je; Kim, Mi-Hyang; Kim, Sung-Gu; Lee, Sang-Hyeon;
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This study was aimed to verify suppressive effect of pine-needle extract on lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression. In order to evaluate suppressive effect on iNOS expression, RAW 264.7 cells were stably transfected using an iNOS promoterluciferase reporter plasmid yielding RAW 264.7/pGL2-NeomiNOS_ pro11 cells. Established in vitro detection system revealed to diminish LPS-induced iNOS expression by 0.1~500 of saponin at the concentration-dependant manner. Pine needle extract also diminished LPS-induced iNOS expression to 92 and 88% at 500 and 50 , respectively. These results suggest that the in vitro detection system developed here could be useful for the verification of suppressive materials on iNOS expression and pine needle extract could be used for the development of functional foods.
inducible nitric oxide synthase (iNOS);luciferase assay;pine needles;RAW 264.7 cells;
 Cited by
톳(Hizikia fusiformis) 에탄올 추출물 및 분획물의 미백활성,전명제;김미향;장혜지;이승우;김재훈;김형석;이상현;

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Moncada, S. (1999) Nitric oxide: discovery and impact on clinical medicine. J. R. Soc. Med. 92: 164-169. crossref(new window)

Nathan, C. (1992) Nitric oxide as a secretory product of mammalian cells. The FASEB journal 6: 3051-3064. crossref(new window)

Andreakos, E., B. Foxwell, and M. Feldmann (2004) Is targeting Toll-like receptors and their signaling pathway a useful therapeutic approach to modulating cytokine-driven inflammation? Immunol. Rev. 202: 250-265. crossref(new window)

Bredt, D. S. and S. H. Snyder (1994) Transient nitric oxide synthase neurons in embryonic cerebral cortical plate, sensory ganglia, and olfactory epithelium. Neuron 13: 301-313. crossref(new window)

Jaffrey, S. R. and S. H. Snyder (1995) Nitric oxide: a neural messenger. Annu. Rev. Cell Dev. Biol. 11: 417-440. crossref(new window)

Tsao, L. T., C. Y. Lee, L. J. Huang, S. C. Kuo, and J. P. Wang (2002) Inhibition of lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 macrophages by a synthetic carbazole, LCY-2-CHO. Biochem. Pharmacol. 63: 1961-1968. crossref(new window)

Vodovotz, Y., D. Russell, Q. W. Xie, C. Bogdan, and C. Nathan (1995) Vesicle membrane association of nitric oxide synthase in primary mouse macrophage. J. Immunol. 154: 2914-2925.

Lowenstein, C. J., E. W. Alley, P. Raval, A. M. Snowman, S. H. Synder, S. W. Russell, and W. J. Murphy (1993) Macrophage nitric oxide synthase gene: two upstream regions mediate induction by interferon gamma and lipopolysaccharide. Proc. Natl. Acad. Sci. USA 90: 9730-9734. crossref(new window)

Chabrier, P. E. and M. Auguest (1999) Nitric oxide synthases: targets for therapeutic strategies in neurological diseases. Cell Mol. Life Sci. 55: 1029-1035. crossref(new window)

Lee, Y. H., S. H. Shin, Y. S. Choi, and S. Y. Lee (1996) Development of the health foods containing the extract from Pinus strobus leave. J. Kor. Soc. Food Nutr. 25: 379-389.

Kim, Y. M., Y. K. Jeong, M. H. W. Y. Lee, and H. I. Rhee (2006) Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition 21: 756-761.

Busserolles, J., E. Gueux, B. Balasinska, Y. Piriou, E. Rock, Y. Rayssiguier, and A. Mazur (2006) In vivo antioxidant activity of procyanidin-rich extracts from grape seed and pine (Pinus maritima) barks in rats. Int. J. Vitam. Nutr. Res. 77: 22-27.

Park, H. G., M. R. Cha, J. H. Hwang, J. Y. Kim, M. S. Park, S. U. Choi, H. R. Park, and Y. I. Hwang (2006) Antimicrobial activity of the extract from Pyrola japonica against Bacillus subtilis. J. Life Sci. 16: 989-993. crossref(new window)

Wei, F. X., M. Y. Li, Y. H. Song, and H. Z. Li (2008) Apoptosis and activity changes of telomerase induced by essential oil from pine needles in HepG2 cell line. Zhong. Yao. Cai. 31: 1197-1200.

Chung, Y. J., M. W. Bae, J. S. Chung, and M. S. Chung (2002) Cytotoxic effect of the distilled pine-needle extracts on several cancer cell lines in vitro. J. Korean Soc. Food Sci. Nutr. 31: 691-695. crossref(new window)

Park, N. H. and S. H. Lee (2003) Antimicrobial activity of pine needle extract and horseradish on the growth of vibrio. J. Korean Soc. Food Sci. Nutr. 32: 185-190. crossref(new window)

Kim, Y. M., Y. K. Jeong, M. H. Wang, W. Y. Lee, and H. I. Rhee (2006) Inhibitory effect of pine extract on alpha-glucosidase activity and postprandial hyperglycemia. Nutrition 21: 756-761.

Kim, N. Y., M. K. Jang, D. G. Lee, K. H. Yu, H. J. Jang, M. H. Kim, S. G. Kim, B. H. Yoo, and S. H. Lee (2010) Comparison of methods for proanthocyanidin extraction from pine (Pinus densiflora) needles and biological activities of the extracts. Nut. Res. and Practice 4: 16-22. crossref(new window)

Kim, N. Y., M. K. Jang, M. J. Jeon, D. G. Lee, H. J. Jang, S. W. Lee, M. H. Kim, S. G. Kim, and S. H. Lee (2010) Verification of antimicrobial activities of various pine needle extracts against antibiotic resistant strains of Staphylococcus aureus. J. Life Sci. 20: 589-596. crossref(new window)

Kim, Y., H. Y. Min, H. J. Park, E. J. Lee, E. J. Park, H. J. Hwang, C. Jin, Y. S. Lee, and S. K. Lee (2004) Suppressive effects of nitric oxide production and inducible nitric oxide synthase (iNOS) gene expression by Calystegia soldanella methanol extract on lipopolysaccharide-activated RAW 264.7 cells. Eur. J. Cancer Prev. 13: 419-424. crossref(new window)

Ryu, D. S., S. H. Kim, and D. S. Lee (2008) Immunomodulating activity of Salicorniaherbacea extract. Kor. J. Microbiol. Biotechnol. 36: 135-141.

Estrada, A., G. S. Katselis, B. Laarveld, and B. Barl (2000) Isolation and evaluation of immunological adjuvant activities of saponins from Polygala senega L. Comparative Immuno. Microbiol. Infectious Diseases 23: 27-43. crossref(new window)

Jeon, H. K., S. C. Kim, and N. P. Jung (1991) Effects of Ginseng Saponin Fraction and Cyclophosphamide on the Tumoricidal Activity of Mouse Macrophage and the Antitumor Effect. Kor. J. Ginseng Sci. 15: 99-105.

Yoon, J. A., K. W. Yu, S. H. Shin, and H. Y. Cho (2010) Activation of intestinal immune system by an orally administered methanol extract from pine needles. J. Kor. Soc. Food Sci. Nutr. 39: 356-362. crossref(new window)

Choi, H. S., D. Hang, S. J. Cho, S. C. Kang, E. S. Sohn, S. P. Lee, S. N. Pyo, and E. W. Son (2006) Immunomodulatory activity of pine needle (Pinus densiflora) extracts in macrophages. J. food Sci. Nutr. 11: 105-109. crossref(new window)