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Optimized Production of Selenocysteine Containing Formate Dehydrogenase H in Escherichia coli
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  • Journal title : KSBB Journal
  • Volume 26, Issue 3,  2011, pp.189-192
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.3.189
 Title & Authors
Optimized Production of Selenocysteine Containing Formate Dehydrogenase H in Escherichia coli
Sa, Young-Seung; Kim, Yong-Hwan;
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 Abstract
Much interest has been recently focused on the production of large quantities of hydrogen, due to its potential importance in our economy and needs in the petroleum and chemical industries. Formate dehydrogenase H (FDH-H) from Escherichia coli containing selenocysteine that oxidizes formate to carbon dioxide with the release of hydrogen is a component of the anaerobic formate hydrogen-lyase complex of E. coli. To make full use of FDH-H, we need effective expression condition. In this approach, we investigated the effect of pH on FDH-H stability and observed the effect of selenite and formate concentration on the activity of FDH-H. Additionally, coexpression of selenocysteine insertion genes were tried to improve the expression of FDH-H. The highest level of FDH-H expression was achieved by coexpression of selenocysteine insertion genes (pSUABC) as well as by the addition of selenite and 10 mM formate. At this optimized condition, a 2.6 fold elevation of expression of FDH-H was achieved.
 Keywords
formate dehydrogenase H;FDH-H;selenocysteine;pSUABC plasmid;
 Language
Korean
 Cited by
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