Advanced SearchSearch Tips
In vitro Biocompatibility Evaluation of Biomaterial-elution Using Inflammatory Cell Lines
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : KSBB Journal
  • Volume 26, Issue 3,  2011, pp.248-254
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.3.248
 Title & Authors
In vitro Biocompatibility Evaluation of Biomaterial-elution Using Inflammatory Cell Lines
Shin, Youn-Ho; Song, Kye-Yong; Seo, Min-Ji; Kim, Sung-Min; Park, Jung-Keug; Kim, Dong-Sup; Park, Ki-Jung; Hur, Chan-Hoi; Cha, Ji-Hun; Seo, Young-Kwon;
  PDF(new window)
Various biometerials have been researched and have been developed for treatment of some disease through transplantation to body. They have been evaluated by in vitro cytotoxicity test using some skin-derived cell lines for prediction of their biocompatibility in vivo. However, the results of experiments using mesenchymal or epithelial cells could not be considered in vivo immune reaction. In this study, we evaluated the biomaterial-elution (elute from high density polyethylene film) using some cell lines (L929, Jurkat, U937) in vitro, and then that results were compared with in vivo results from guinea pig sensitization test. In sensitization test, saline and elution of syringe could not induce erythema, but only DNCB (hypersensitive chemical) induce erythema at guinea pig sensitization test. In cell experiment, the cytotoxicity results of inflammatory cells (Jurkat; T lymphocyte, U937; monocyte) was no difference with L929 (fibroblast) in the overall trend. However, inflammatory cell lines were only secreted inflammatory cytokine (TNF-, INF-) in some materials (biomateriallution, FAC, DNCB). And the biomaterial-elution did not have toxicity to the cells, but it induced the inflammatory cytokines in inflammatory cell lines only. So, we were predicted inflammatory reaction through the cytokine resultes of inflammatory cell lines, and it was more correlated with in vivo results than cytotoxicity test. Therefore, we suggested that the inflammatory cytokine assay using inflammatory cell lines are more effective method in vitro for evaluation of biocompatibility of biomaterials or chemicals.
inflammation;biocompatibility;inflammatory cell line;fibroblast;cytokine;
 Cited by
Wikipedia, the free encyclopedia.

Kimber, I., J. S. Pichowski, and C. J. Betts, et al. (2001) Alternative approaches to the identification and characterization of chemical allergens. Toxicol. In Vitro 15: 307-312. crossref(new window)

Steiling, W., D. Basketter, and K. Berthold, et al. (2001) Skin sensitisation testing-new perspectives and recommendations. Food Chern. Toxicol. 39: 293-301. crossref(new window)

Kay, A. B. (2000) Overview of 'allergy and allergic diseases: with a view to the future'. Br. Med. Bull. 56: 843-864. crossref(new window)

Cavani, A., C. Albanesi, and C. Traidl, et al. (2001) Effector and regulatory T cells in allergic contact dermatitis. Trends Immunol. 22: 118-120. crossref(new window)

Buehler, E. V. (1965) Delayed Contact Hypersensitivity in the Guinea Pig. Arch. Dermatol. 91: 171-177. crossref(new window)

Magnusson, B. and A. M. Kligman (1970) Allergic contact dermatitis in the guinea pig. Illinois: Charles C. Thomas. Springfield.

Gad, S. C., B. J. Dunn, and D. W. Dobbs, et al. (1986) Development and validation of an alternative dermal sensitization test: the mouse ear swelling test (MEST). Toxieol. Appl. Pharmacol. 84: 93-114. crossref(new window)

Kimber, I., R. J. Dearman, and E. W. Scholes, et al. (1994) The local lymph node assay: developments and applications. Toxicology 93: 13-31. crossref(new window)

GECD guideline for testing of chemicals -skin sensitisation. 1992.

Sandoe, P., S. B. Christiansen, and M. C. Appleby (2003) Farm animal welfare: the interaction of ethical questions and animal welfare science. Animal Welfare 12: 10.

Collins, F. S., G. M. Gray, and J. R. Bucher (2008) Toxicology. Transforming environmental health protection. Science 319: 906-907. crossref(new window)

Martin, S. F., P. R. Esser, and S. Schmucker, et al. (2010) T-cell recognition of chemicals, protein allergens and drugs: towards the development of in vitro assays. Cell. Mol. Life Sci. 67: 4171-4184. crossref(new window)

Krasteva, M., J. Peguet-Navarro, and C. Moulon, et al. (1996) In vitro primary sensitization of hapten-specific T cells by cultured human epidermal Langerhans S. F. Martin et al cells - a screening predictive assay for contact sensitizers. Clin. Exp. Allergy 26: 563-570. crossref(new window)

Rougier, N., G. Redziniak, and D. Mougin, et al. (2000) In vitro evaluation of the sensitization potential of weak contact allergens using Langerhans-like dendritic cells and autologous T cells. Toxicology 145: 73-82. crossref(new window)

Moed, H., M. von Blomberg, and D. P. Bruynzeel, et al. (2005) Improved detection of allergen-specific T-cell responses in allergic contact dermatitis through the addition of 'cytokine cocktails'. Exp. Dermatol. 14: 634-640. crossref(new window)

Song, K. Y., M. K. Kim, and J. G. Chi (2007) Core Pathology. 2nd ed., pp. 45-69. Korea Medical Book, Korea.

Barbara, J. A., X. Van ostade, and A. Lopez (1996) Tumour necrosis factor-alpha (TNF -alpha): the good, the bad and potentially very effective. Immunol. Cell Biol. 74: 434-443. crossref(new window)

Schroder, K., P. J. Hertzog, and T. Ravasi, et al. (2004) Interferongamma: an overview of signals, mechanisms and functions. J. Leukoc. Biol. 75: 163-189.

Masui, T., S. Sakano, and Y Hasegawa, et al. (2005) Expression of inflammatory cytokines, RANKL and OPG induced by titanium, cobalt-chromium and polyethylene particles. Biomaterials 26: 1695-1702. crossref(new window)

Bosetti, M., L. Zanardi, and P. Bracco, et al. (2003) In vitro evaluation of the inflammatory activity of ultra-high molecular weight polyethylene. Biomaterials 24: 1419-1426. crossref(new window)

Holding, C. A., D. M. Findlay, and R. Stamenkov, et al. (2006) The correlation of RANK, RANKL and TNFalpha expression with bone loss volume and polyethylene wear debris around hip implants. Biomaterials 27: 5212- 5219. crossref(new window)

Warashina, H., S. Sakano, and S. Kitamura, et al. (2003) Biological reaction to alumina, zirconia, titanium and polyethylene particles implanted onto murine calvaria. Biomaterials 24: 3655-3661. crossref(new window)