JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Preparation of Alginate-fibroin Beads with Diverse Structures
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : KSBB Journal
  • Volume 26, Issue 5,  2011, pp.422-426
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.5.422
 Title & Authors
Preparation of Alginate-fibroin Beads with Diverse Structures
Lee, Jin-Sil; Lee, Shin-Young; Hur, Won;
  PDF(new window)
 Abstract
Alginate bead has been supplemented with various polymers to control permeability and to enhance mechanical strength. In this report, fibroin-reinforced alginate hydrogel was prepared, in which spatial localization of fibroin molecules was investigated. Confocal laser scanning microscopy revealed that fibroin molecules formed a fibrous network in the alginate-fibroin beads, which was expected to enhance mechanical strength as same as in many composite materials. Uniaxial compression test showed that fibroin-reinforced alginate beads had increased mechanical strength only after methanol treatment that caused -sheet formation among fibroin molecules. Simultaneous curing and dialysis of alginate beads were carried out to remove excesscalcium but to retain fibroin in the dialysis chamber, which fabricated beads without internal fibrous fluorescent stains. Fibroin molecules were only found beneath the surface of the beads. The fibroin-diffused shell was further processed to form a thick wall after drying or was mobilizedto the centre of the bead by methanol treatment. Accordingly, the structure analyses provide processing methods of fibroin to form a wall or center clumps, which could be applied to design controlled delivery device.
 Keywords
alginate bead;fibroin;wall material;methanol treatment;core-shell;
 Language
Korean
 Cited by
 References
1.
Tam, S. K., J. Dusseault, S. Bilodeau, G. Langlois, J. Hallé, and L. Yahia (2011) Factors influencing alginate gel biocompatibility. J. Biomed. Mater. Res. A 98A: 40-52. crossref(new window)

2.
Krasaekoopt, W., B. Bhandari, and H. Deeth (2004) The influence of coating materials on some properties of alginate beads and survivability of microencapsulated probiotic bacteria. Int. Dairy J. 14: 737-743. crossref(new window)

3.
Silva, C. M., A. J. Ribeiro, M. Figueiredo, D. Ferreira, and F. Veiga (2006) Microencapsulation of hemoglobin in chitosancoated alginate microspheres prepared by emulsification/internal gelation. AAPS J. 7: E903-913.

4.
Sahasathian, T., N. Praphairaksit, and N. Muangsin (2010) Mucoadhesive and floating chitosan-coated alginate beads for the controlled gastric release of amoxicillin. Arch. Pharm. Res. 33: 889-899. crossref(new window)

5.
Pongjanyakul, T. and T. Rongthong (2010) Enhanced entrapment efficiency and modulated drug release of alginate beads loaded with drugclay intercalated complexes as microreservoirs. Carbohydr. Polym. 81: 409-419. crossref(new window)

6.
Stancu, I., D. M. Dragusin, E. Vasile, R. Trusca, I. Antoniac, and D. S. Vasilescu (2011) Porous calcium alginate-gelatin interpenetrated matrix and its biomineralization potential. J. Mater. Sci. Mater. Med. 22: 451-460. crossref(new window)

7.
Kong, H. J. and D. J. Mooney (2003) The effects of poly (ethyleneimine) (PEI) molecular weight on reinforcement of alginate hydrogels. Cell Transplant. 12: 779-785. crossref(new window)

8.
Altman, G. H., F. Diaz, C. Jakuba, T. Calabro, R. L. Horan, J. Chen, H. Lu, J. Richmond, and D. L. Kaplan (2003) Silk-based biomaterials. Biomaterials 24: 401-416. crossref(new window)

9.
Sashina, E., A. Bochek, N. Novoselov, and D. Kirichenko (2006) Structure and solubility of natural silk fibroin. Russian J. Appl. Chem. 79: 869-876. crossref(new window)

10.
Barbetta, A., E. Barigelli, and M. Dentini (2009) Porous alginate hydrogels: synthetic methods for tailoring the porous texture. Biomacromolecules 10: 2328-2337. crossref(new window)

11.
Bernhardt, A., F. Despang, A. Lode, A. Demmler, T. Hanke, and M. Gelinsky (2009) Proliferation and osteogenic differentiation of human bone marrow stromal cells on alginate-gelatinehydroxyapatite scaffolds with anisotropic pore structure. J. Tissue Eng. Regen. Med. 3: 54-62. crossref(new window)

12.
Zmora, S., R. Glicklis, and S. Cohen (2002) Tailoring the pore architecture in 3-D alginate scaffolds by controlling the freezing regime during fabrication. Biomaterials 23: 4087-4094. crossref(new window)

13.
Wilson, D., R. Valluzzi, and D. Kaplan (2000) Conformational transitions in model silk peptides. Biophys. J. 78: 2690-2701. crossref(new window)

14.
Melekaslan, D., N. Gundogan, and O. Okay (2003) Elasticity of poly(acrylamide) gel beads. Polym. Bull. 50: 287-294.

15.
Chan, E., T. Lim, W. Voo, R. Pogaku, B. T. Tey, and Z. Zhang (2011) Effect of formulation of alginate beads on their mechanical behavior and stiffness. Particuology 9: 228-234. crossref(new window)

16.
Mandal, B. B. and S. C. Kundu (2009) Calcium alginate beads embedded in silk fibroin as 3D dual drug releasing scaffolds. Biomaterials 30: 5170-5177. crossref(new window)

17.
Wang, X., E. Wenk, X. Hu, G. R. Castro, L. Meinel, X. Wang, C. Li, H. Merkle, and D. L. Kaplan (2007) Silk coatings on PLGA and alginate microspheres for protein delivery. Biomaterials 28: 4161-4169. crossref(new window)