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Optimization for Production of Exo-β-1,3-glucanase (Laminarinase) from Aspergillus oryzae in Saccharomyces cerevisiae
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  • Journal title : KSBB Journal
  • Volume 26, Issue 5,  2011, pp.427-432
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.5.427
 Title & Authors
Optimization for Production of Exo-β-1,3-glucanase (Laminarinase) from Aspergillus oryzae in Saccharomyces cerevisiae
Kim, Min-Jung; Nam, Soo-Wan; Tamano, Koichi; Machida, Masayuki; Kim, Sung-Koo; Kim, Yeon-Hee;
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 Abstract
In this study, a EXGA gene code for exo-β-1,3-glucanase from Aspergillus oryzae was overexpressed and secretory produced in Saccharomyces cerevisiae. To overexpress the β-1,3-glucanase, pGInu-exgA and pAInu-exgA plasmids having GAL10 and ADH1 promoter, respectively, and exoinulinase signal sequence (Inu s.s) were constructed and introduced in S. cerevisiae SEY2102 and 2805. The recombinant β-1,3-glucanase was successfully expressed and secreted into the medium and the β--1,3-glucanase activity in 2102/pGInu-exgA and 2102/pAInu-exgA strain were 5.01 unit/mL and 4.09 unit/mL, respectively. In the 2805/pGInu-exgA and 2805/pAInu-exgA strain, the β-1,3-glucanase activity showed 3.23 unit/mL and 3.22 unit/mL, respectively. Secretory efficiency in each strain reached 95% to 98%. Subsequently, the recombinant β1,3-glucanase was used for ethanol production. Ethanol productivity in 2102/pAInu-exgA strain was 0.83 g/L when pre-treated Laminaria japonica which has initial reducing sugar of 1.4 g/L was used as substrate. It is assumed that the polysaccharides of Laminaria japonica was effectively saccharified by recombinant β-1,3-glucanase, resulting in increase of ethanol productivity. These results suggested that recombinant β-1,3-glucanase was efficiently overexpressed and secreted in S. cerevisiae SEY2102 as host strain by using ADH1 promoter-Inu s.s system.
 Keywords
β1,3-glucanase;ADH1 promoter;GAL10 promoter;Aspergillus oryzae;Saccharomyces cerevisiae;
 Language
Korean
 Cited by
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Xylitol 생산에 최적화된 xylose reductase (GRE3)의 분비발현 시스템,정회명;김재운;김연희;

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Enhancement of β-1,3-Glucanase Activity by Sequential δ-Sequence Mediated Integration in Saccharomyces cerevisiae, Journal of Life Science, 2014, 24, 10, 1046  crossref(new windwow)
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Construction of an expression system for the secretory production of recombinant α-agarase in yeast, Biotechnology Letters, 2012, 34, 6, 1041  crossref(new windwow)
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