Advanced SearchSearch Tips
Production of Viable Lactobacillus crispatus by Using Whey Based Medium
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : KSBB Journal
  • Volume 26, Issue 6,  2011, pp.529-532
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.6.529
 Title & Authors
Production of Viable Lactobacillus crispatus by Using Whey Based Medium
Chang, Chung-Eun; Koo, Ja-Ryong; So, Jae-Seong; Yun, Hyun-Shik;
  PDF(new window)
Whey based medium was optimized for the production of viable Lactobacillus crispatus KLB 46 isolated from the vagina of Korean women. Among the various nitrogen sources such as yeast extract, beef extract, and proteose peptone no. 3 supplemented to whey, beef extract showed the highest viable cell production. The addition of Tween 80 to the whey based medium increased viable cell concentration. As beef extract supplementation is not economically attractive, corn steep liquor was added as a supplementary nitrogen sources. When corn steep liquor was supplied with beef extract with the ratio 5 : 1, the viable cell count was CFU/mL. Also, the addition of mineral salts containing sodium acetate (5 g/L), potassium phosphate dibasic (2 g/L), magnesium sulfate (0.1 g/L) and manganese sulfate (0.05 g/L) to the whey medium increased viable cell count further ( CFU/mL).
Lactobacillus crispatus;cheese whey;media optimization;viable cell;
 Cited by
Eschenbach, D. A., Davick, P. R., Williams, B. L., Klebanoff, S. J., Young-Smith, K., Critchlow, C. M., and K. K. Holmes (1989) Prevalence of hydrogen peroxide-producing Lactobacillus species in normal women and women with bacterial vaginosis. J. Clinical Microbiol. 27: 251-256.

Hughes, V. L. and S. L. Hillier (1990) Microbiologic characteristics of Lactobacillus products used for colonization of the vagina. Obstet. Gynecol. 75: 244-248.

Sobel, J. D. (1989) Vaginal infections in adult women. Med. Clinical North America 74: 1578-1602.

Chang, C. E., S. C. Kim, J. S. So, and H. S. Yun (2001) Cultivation of Lactobacillus crispatus KLB 46 isolated from human vagina. Biotechnol. Bioprocess Eng. 6: 128-132. crossref(new window)

Parent, D., Bossens, M., Bayot, D., Kirkpatrick, C., Graf, F., Wilkinson, F. E., and R. R. Kaisser (1996) Therapy of bacterial vaginosis using exogenously-applied Lactobacilli Acidophili and a low dose of Estriol a placebo-controlled multicentric clinical trial. Arzne-Fors/Drug Res. 46: 68-73.

Lund, B., Norddahl, B., and B. Ahring (1992) Production of lactic acid from whey using hydrolysed whey protein as nitrogen source. Biotechnol. Lett. 14: 851-856. crossref(new window)

Amrane, A. and Y. Prigent (1998) Lactic acid production rates during the different growth phases of Lactobacillus helveticus cultivated on whey supplemented with yeast extract. Biotechnol. Letters 20: 379-383. crossref(new window)

Arasaratnam, V., Senthuran, A., and K. Balasubramaniam (1996) Supplementation of with glucose and different nitrogen sources for lactic acid production by Lactobacillus delbrueckii. Enz. Microbial Technol. 19: 482-486. crossref(new window)

De Man, J. C., Rogosa, M., and M. E. Sharpe (1960) A medium for the cultivation of Lactobacilli. J. Appl. Bacteriol. 23: 130-135. crossref(new window)