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Isolation and Characteristics of Alginate lyase Producing Microorganism: Sanguibacter keddieii NC9
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  • Journal title : KSBB Journal
  • Volume 26, Issue 6,  2011, pp.538-542
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.6.538
 Title & Authors
Isolation and Characteristics of Alginate lyase Producing Microorganism: Sanguibacter keddieii NC9
Kim, Geun-Hyub; Lee, Sung-Mok; Choi, Soo-Jeong; Lee, Jae-Hwa;
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 Abstract
The research was purposed production of oligosaccharide from alginate hydrolysis the main composition in cell walls of sea weed. We was isolated 252 strains from sea water and mud flat, the highest alginate lyase activity was selected, and identified as Sanguibacter keddieii NC9 by 16S rDNA sequence analysis. In this study was select the sodium alginate concentration, pH, temperature for the production of alginate lyase activity. Alginate lyase activity was confirmed from plate assay with 10% cetylpyridinium chloride. The optimum culture conditions for the production of alginate lyase were sodium alginate 10 g/L, peptone 5 g/L, , pH 9 and 36 hours incubation time. Sanguibacter keddieii NC9, its alginate lyase would be useful for the production of bioenergy and biofunctional oligosaccharides from sea weed.
 Keywords
Alginate lyase;sea water;enzymatic activity;brown algae;
 Language
Korean
 Cited by
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