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Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase
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  • Journal title : KSBB Journal
  • Volume 26, Issue 6,  2011, pp.552-556
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.6.552
 Title & Authors
Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase
Jang, Hye-Ji; Lee, Dong-Geun; Lee, Seung-Woo; Jeon, Myong-Je; Chun, Won-Ju; Kwon, Kae-Kyoung; Lee, Hee-Soon; Lee, Sang-Hyeon;
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 Abstract
A novel agar-degrading bacterium mbrc-1 was isolated from seashore of Kyungpo at Gangwon province and cultured in marine broth 2216 medium. Isolated bacterium mbrc-1 was named as Flammeovirga sp. mbrc-1 based on the 16S rDNA sequence. Its agarase showed maximum activity of 923 units/L at pH 7.0 and and sustained 90% remaining activity after exposed to for 2 hours. The enzyme hydrolyzed agarose to yield neoagarohexaose (18.5%), neoagarotetraose (38%) and neoagarobiose (43.5%), indicating that the enzyme is -agarase. Thus, isolated bacterium and its -agarase would be useful for the industrial production of neoagarotetraose and neoagarobiose.
 Keywords
-agarase;agarose;Flammeovirga sp.;neoagarobiose;neoagarotetraose;
 Language
Korean
 Cited by
1.
유자즙 첨가량을 달리한 마요네즈 저장 중 품질특성,김경미;이종은;김진숙;최송이;장영은;

한국식품저장유통학회지, 2014. vol.21. 6, pp.799-807 crossref(new window)
2.
신규 한천분해세균 Maribacter sp. SH-1의 분리 및 효소 특성조사,이창은;이솔지;이동근;이상현;

한국미생물생명공학회지, 2016. vol.44. 2, pp.156-162 crossref(new window)
3.
해양성 Marinomonas sp. SH-2 균주가 생성하는 agarase의 분리 및 특성조사,조정권;이솔지;이동근;이상현;

생명과학회지, 2016. vol.26. 2, pp.198-203 crossref(new window)
4.
분리된 Simiduia sp. SH-4가 생산하는 β-agarase의 특성조사,김재덕;이솔지;조정권;이동근;이상현;

생명과학회지, 2016. vol.26. 4, pp.453-459 crossref(new window)
1.
Industrial Applications of Saccharification Technology for Red Seaweed Polysaccharide, KSBB Journal, 2014, 29, 5, 307  crossref(new windwow)
2.
Characterization of α-agarase from Alteromonas sp. SH-1, KSBB Journal, 2016, 31, 2, 113  crossref(new windwow)
 References
1.
Duckworth, M. and W. Yaphe (1971) Structure of agar. I. Fractionation of a complex mixture of polysaccharides. Carbohyd. Res. 16: 189-197. crossref(new window)

2.
Hong, J.-H., J.-J. Lee, S.-H. Hur, H. Choi, and J.-Y. Kong (2001) Effect of agarooligosaccharides on the growth of intestinal bacteria. J. Food Hyg. Safety 16: 11-15.

3.
Do, J.-H. (1997) Extraction and purification of agar from Gelidium amansii. J. Kor. Fish. Soc. 30: 423-427.

4.
Yang, J.-I., L.-C. Chen, Y.-Y. Shih, C. Hsieh, C.-Y. Chen, W.-M. Chen, and C.-C. Chen (2011) Cloning and characterization of ${\beta}$-agarase AgaYT from Flammeovirga yaeyamensis strain YT. J. Biosci. Bioeng. 112: 225-232. crossref(new window)

5.
Potin, P., C. Richard, C. Rochas, and B. Kliarg (1993) Purification and characterization of the ${\alpha}$-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B. Eur. J. Biochem. 214: 599-607. crossref(new window)

6.
Kirimura, K., N. Masuda, Y. Iwasaki, H. Nakagawa, R. Kobayashi, and S. Usami (1999) Purification and characterization of a novel ${\beta}$-agarase from an alkalophilic bacterium, Alteromonas sp. E-1. J. Biosci. Bioeng. 87: 436-441. crossref(new window)

7.
Schafer, H. (2007) Isolation of Methylophaga spp. from marine dimethylsulfide-degrading enrichment cultures and identification of polypeptides induced during growth on dimethylsulfide. Appl. Environ. Microbiol. 73: 2580-2591. crossref(new window)

8.
Suzuki, H., Y. Sawai, T. Suzuki, and K. Kawai (2002) Purification and characterization of an extracellular ${\alpha}$neoagarooligosaccharide hydrolase from Bacillus sp. MK03. J. Biosci. Bioeng. 93: 456-463. crossref(new window)

9.
Park, G. T., D. G. Lee, N. Y. Kim, E. J. Lee, J. G. Jung, J. H. Lee, M. S. Heo, and S. H. Lee (2005) Isolation and characterization of a marine bacterium producing thermotolerant agarase. J. Life Sci. 15: 884-888. crossref(new window)

10.
Lee, D.-G., N. Y. Kim, M. K. Jang, and S.-H. Lee (2007) Isolation and characterization of a marine bacterium Thalassomonas sp. SL-5 producing ${\beta}$-agarase. J. Life Sci. 17: 70-75. crossref(new window)

11.
Lee, D.-G., O.-H. Lee, H. J. Jang, M.-K. Jang, K. H. Yoo, and S.-H. Lee (2008) Isolation and characterization of a marine derived bacterium Glaciecola sp. SL-12 producing ${\beta}$-agarase. J. Life Sci. 18: 58-62. crossref(new window)

12.
Jang, M.-K., O. H. Lee, K. H. Yoo, D.-G. Lee, and S.-H. Lee (2007) Secretory overexpression of ${\beta}$-agarase in Bacillus subtilis and antibacterial activity of enzymatic products. J. Life Sci. 17: 1601-1604. crossref(new window)

13.
Yoshizawa, Y., A. Ametani, J. Tsunehiro, K. Nomura, M. Itoh, F. Fukui, and S. Kaminogawa (1995) Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structure-function relationships and improved solubility. Biosci. Biotechnol. Biochem. 59: 1933-1937. crossref(new window)

14.
Lee, D.-G., G.-T. Park, N. Y. Kim, E.-J. Lee, M. K. Jang, Y. G. Shin, G.-S. Park, T.-M. Kim, J.-H. Lee, J.-H. Lee, S.-J. Kim, and S.-H. Lee (2006) Cloning, expression, and characterization of a glycoside hydrolase family 50 ${\beta}$-agarase from marine Agarivorans isolate. Biotechnol. Lett. 28: 1925-1932. crossref(new window)

15.
Kobayashi, R., M. Takisada, T. Suzuki, K. Kirimura, and S. Usami (1997) Neoagarobiose as a novel moisturizer with whitening effect. Biosci. Biotechnol. Biochem. 61: 162-163. crossref(new window)

16.
Jang, M.-K., D.-G. Lee, N.-Y. Kim, K.-H. Yu, H. J. Jang, S. W. Lee, H. J. Jang, Y. J. Lee, and S.-H. Lee (2009) Purification and characterization of neoagarotetraose from hydrolyzed agar. J. Microbiol. Biotechnol. 19: 1197-1200.

17.
Duckworth, M. and W. Yaphe (1970) Thin-layer chromatographic analysis of enzymic hydrolysate of agar. J. Chromatogr. 49: 482-487. crossref(new window)

18.
Groleau, D. and W. Yaphe (1977) Enzymatic hydrolysis of agar: purification and characterization of ${\beta}$-neoagarotetraose hydrolase from Peudomonas atlantica. Can. J. Microbiol. 23: 672-679. crossref(new window)

19.
Shoichi, H. and Y. Akira (2007) Flammeovirga kamogawensis sp. nov., isolated from coastal seawater in Japan. Int. J. Syst. Evol. Microbiol. 57: 1327-1330. crossref(new window)

20.
Takshashi, M., K. Suzuki, and Y. Nakagawa (2006) Emendation of the genus Flammeovirga and Flammeovirga aprica with the proposal of Flammeovirga arenaria nom. rev., comb. nov. and Flammeovirga yaeyamensis sp. nov. Int. J. Syst. Evol. Microbiol. 56: 2095-2100. crossref(new window)

21.
Kim, B.-J., S.-H. Hwang, H.-J. Kim, Y.-S. Kang, S.-D. Ha, and J.-Y. Kong (1999) Characteristics of ${\beta}$-agarase produced by marine bacterium Bacillus cereus ASK202. Kor. J. Biotechnol. Bioeng. 14: 96-102.

22.
Jung, I. S., Y. J. Kim, H. J., Song, S. W. Gal, and Y. J. Choi (2008) Purification and properties of a novel extracellular agarase from marine bacterium, Sphingomonas paucimobilis AS-1. J. Life Sci. 18: 103-108. crossref(new window)

23.
Araki, T., Z. Lu, and T. Morishita (1998) Optimization of parameters for isolation of protoplasts from Gracilaria verrucosa (Rhodophyta). J. Mar. Biotechnol. 6: 193-197.

24.
Ohta, Y., Y. Hatada, M. Miyazaki, Y. Nogi, S. Ito, and K. Horikoshi (2005) Purification and characterization of a novel alpha-agarase from a Glaciecola sp. Curr. Microbiol. 50: 212-216. crossref(new window)

25.
Yoshizawa, Y., A. Ametani, J. Tsunehiro, K. Nomura, M. Itoh, F. Fukui, and S. Kaminogawa (1995) Macrophage stimulation activity of the polysaccharide fraction from a marine alga (Porphyra yezoensis): structurefunction relationships and improved solubility. Biosci. Biotechnol. Biochem. 59: 1933-1939. crossref(new window)

26.
Lee, S. A., J. U. Kim, J. G. Jung, I. H. Kim, S. H. Lee, S. J. Kim, and J. H. Lee (2006) Production of ${\beta}$-agarase in batch and fed-batch culture by Agarivorans sp. JA-1. Kor. J. Biotechnol. Bioeng. 21: 389-393.

27.
Kong, J. Y., S. K. Bae, S. H. Hwang, S. D. Ha, H. T. Kim, S. K. Kim, and B. J. Kim (1996) Purification of extracellular agarase from marine bacterium (Pseudomonas sp. W7) and molecular cloning of the agarase gene. Kor. J. Biotechnol. Bioeng. 11: 37-45.