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Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase
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  • Journal title : KSBB Journal
  • Volume 26, Issue 6,  2011, pp.552-556
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2011.26.6.552
 Title & Authors
Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase
Jang, Hye-Ji; Lee, Dong-Geun; Lee, Seung-Woo; Jeon, Myong-Je; Chun, Won-Ju; Kwon, Kae-Kyoung; Lee, Hee-Soon; Lee, Sang-Hyeon;
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A novel agar-degrading bacterium mbrc-1 was isolated from seashore of Kyungpo at Gangwon province and cultured in marine broth 2216 medium. Isolated bacterium mbrc-1 was named as Flammeovirga sp. mbrc-1 based on the 16S rDNA sequence. Its agarase showed maximum activity of 923 units/L at pH 7.0 and and sustained 90% remaining activity after exposed to for 2 hours. The enzyme hydrolyzed agarose to yield neoagarohexaose (18.5%), neoagarotetraose (38%) and neoagarobiose (43.5%), indicating that the enzyme is -agarase. Thus, isolated bacterium and its -agarase would be useful for the industrial production of neoagarotetraose and neoagarobiose.
-agarase;agarose;Flammeovirga sp.;neoagarobiose;neoagarotetraose;
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