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Optimization of gamma-Aminobutyric Acid Bioconversion by Recombinant Escherichia coli
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  • Journal title : KSBB Journal
  • Volume 27, Issue 2,  2012, pp.127-130
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2012.27.2.127
 Title & Authors
Optimization of gamma-Aminobutyric Acid Bioconversion by Recombinant Escherichia coli
Le Vo, Tam Dinh; Hong, Soon-Ho;
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 Abstract
In this study, the effects of pH, temperature, IPTG concentration and substrate (MSG) concentration on gamma-aminobutyric acid (GABA) production in engineered Escherichia coli were investigated. Glutamate decarboxylase and glutamate/GABA antiporter were overexpressed in GABA aminotransferase knock-out strain for GABA production. The result of optimization study showed the GABA bioconversion was optimized at pH 3.5, , 0.5 mM IPTG, 10 g/L MSG. At this condition, 5.23 g/L of final GABA concentration of was achieved from 10 g/L of MSG, which corresponded to a GABA yield of 85.77%.
 Keywords
gamma-Aminobutyric acid;Escherichia coli;Bioconversion;Optimization;
 Language
Korean
 Cited by
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Gamma-aminobutyric acid production through GABA shunt by synthetic scaffolds introduction in recombinant Escherichia coli, Biotechnology and Bioprocess Engineering, 2016, 21, 2, 261  crossref(new windwow)
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