JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Anti-inflammatory Effect of Onion (Allium cepa) Peel Hot Water Extract in vitro and in vivo
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : KSBB Journal
  • Volume 30, Issue 4,  2015, pp.148-154
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2015.30.4.148
 Title & Authors
Anti-inflammatory Effect of Onion (Allium cepa) Peel Hot Water Extract in vitro and in vivo
Kang, Bo-Kyeong; Kim, Koth-Bong-Woo-Ri; Ahn, Na-Kyung; Choi, Yeon-Uk; Kim, Min-ji; Bark, Si-Woo; Pak, Won-Min; Kim, Bo-Ram; Park, Ji-Hye; Bae, Nan-Young; Ahn, Dong-Hyun;
  PDF(new window)
 Abstract
Onion (Allium cepa) is one of the flavonoids-rich materials in human diet and onion peel, which is the onion by-products, contains over 20 times more quercetin than the flesh. In this study, to examine the anti-inflammatory effects of onion peel hot water extract (OPHWE), the cell viability, nitric oxide (NO), pro-inflammatory cytokines, such as interluekin-6 (IL-6), tumor necrosis factor- (TNF-), and IL-, were measured using the murine macrophage cell line RAW 264.7 cells. The Balb/c mice were used for an in vivo acute toxicity test and ICR mice were used for measurement of inhibition effects of croton oil-induced mouse ear edema. As a result, NO levels decreased in a dose-dependent manner. The production of IL-6, TNF-, and IL- was suppressed by 38%, 41%, and 34% respectively, compared with that of the LPS only group, without any cytotoxicity. The edema formation in the ICR mouse ear was also reduced compared to that in control. Moreover, there were no mortalities occurred in mice administered 5,000 mg/kg body weight of OPHWE. These results suggest that OPHWE has considerable anti-inflammatory activities and can be regarded as a potent candidate material to treat inflammatory diseases.
 Keywords
Onion peel hot water extract;Anti-inflammatory effect;Cytokine;Mouse ear edema;
 Language
Korean
 Cited by
1.
LPS로 유도된 RAW 264.7 세포와 마우스 귀 조직에 대한 양파(Allium cepa L.) 껍질 에탄올 추출물의 항염증 효과,안나경;강보경;김꽃봉우리;김민지;배난영;박지혜;박선희;안동현;

한국식품영양과학회지, 2015. vol.44. 11, pp.1612-1620 crossref(new window)
 References
1.
Ferero-Miliani, L., O. H. Nielsen, P. S. Andersen, and S. E. Girardin (2007) Chronic inflammation: importance of NOD2 and NALP3 in interleukin-1beta generation. Clin. Exp. Immunol. 147: 227-235.

2.
Medzhitov, R (2008) Origin and physiological roles of inflammation. Nature 454: 428-435. crossref(new window)

3.
Davies, P., P. J. Bailey, M. M. Goldenberg, and A. W. Ford-Hutchinson (1984) The role of arachidonic acid oxygenation products in pain and inflammation. Annu, Rev. Immunol. 2: 335-357. crossref(new window)

4.
Pestka, J. and H. R. Zhou. (2006) Toll-like receptor priming sensitizes macrophages to proinflammatory cytokine gene induction by deoxynivalenol and other toxicants. Toxicol. Sci. 92: 445-455. crossref(new window)

5.
Baldwin, A. S. Jr. (1996) The NF-kappa B and I kappa B proteins: new discoveries and insights. Annu. Rev. Immunol. 14: 649-683. crossref(new window)

6.
Giuliani, C., G. Napolitano, I. Bucci, V. Montani, and F. Monaco (2001) NF-${\kappa}B$ transcription factor: role in the pathogenesis of inflammatory, autoimmune, and neoplastic diseases and therapy implications. Clin. Ter. 152: 249-253.

7.
Ding, X., Y, Hiraku, N. Ma, T. Kato, K. Saito, M. Nagahama, R. Semba, K. Kuribayashi, and S. Kawanishi (2005) Inducible nitric oxide synthase-dependent DNA damage in mouse model of inflammatory bowel disease. Cancer Sci. 96: 157-163. crossref(new window)

8.
Majdalawieh, A and H. S. Ro (2010) Regulation of IκBα function and NF-${\kappa}B$ signaling: AEBP1 is a novel proinflammatory mediator in macrophages. Mediators Inflamm. 2010: 1-27.

9.
Tak, P. P and G. S. Firestein (2001) NF-${\kappa}B$: A key role in inflammatory diseases. J. Clin. Invest. 107: 7-11. crossref(new window)

10.
Hippeli, S and E. F. Elastner (1999) Inhibition of biochemical model reactions for inflammatory processes by plant extracts; a review on recent developments. Free Radic. Res. 31: 81-87. crossref(new window)

11.
Iwalewa, E. O., L. J. McGaw, V. Naidoo, and J. N. Eloff (2007) Inflammation: the foundation of diseases and disorders. A review of phytomedicines of south African origin used to treat pain and inflammatory conditions. Afr. J. Biotechnol. 6: 2868-2885.

12.
Yoon, Y. I., M. Y. Chung, J. S. Hwang, T. W. Goo, M. Y. Ahn, Y. B. Lee, M. S. Han, and E. Y. Yun (2014) Anti-inflammatory effect of Oxya chinensis sinuosa ethanol extract in LPS-induced RAW 264.7 cells. J. Life Sci. 24: 370-376. crossref(new window)

13.
Sheo, H. J. and D. L. Jung (1993) Effects of onion juice on toxicity of lead in rat. J. Korean Soc. Food Nutr. 22: 138-143.

14.
Lakhanpal,, P. and D. K. Rai (2007) Quercetin: a versatile flavonoid. I. J. M. U. 2: 22-37.

15.
Bang, H. A. and J. S. Cho (1998) Antioxidant effects on various solvent extracts from onion peel and onion flesh. J. Korean Diet. Assoc. 4: 14-19.

16.
Jeon, S. Y., J. H. Baek, Jeong, E. J. and Y. J. Cha (2012) Potential of onion peel extract as a functional ingredient for functional foods. J. Life Sci. 22: 1207-1213. crossref(new window)

17.
Son, J. Y., H. S. Son, and W. D. Cho (1998) Antioxidant effect of onion skin extract. Korean J. Food Cookery Sci. 14: 16-20.

18.
Ra, K. S., S. H. Chung, H. J. Suh, J. Y. Son, and H. K. Lee (1998) Inhibitor of xanthine oxidase from onion skin. Korean J. Food Sci. Technol. 30: 697-701.

19.
Lee, H. J., K. H. Lee, Y. J. Cha, E. Park, and M. J. Shin (2010) Effects of onion peel extracts on blood circulation in male smokers. J. Korean Soc. Food Sci. Nutr. 39: 1790-1799. crossref(new window)

20.
Eloff, J. N. (1998) Which extractant should be used for the screening and isolation of antimicrobial components from plants? J. Ethnopharmacol. 60: 1-8. crossref(new window)

21.
Park, Y. M., J. H. Won, K. J. Yun, J. H. Ryu, Y. N. Han, S. K. Choi, and K. T. Lee (2006) Preventive effect of Ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression nuclear factor-${\kappa}B$ in RAW 264.7 cells. Biol. Pharm. Bull. 29: 985-990. crossref(new window)

22.
Lee, S. T., Y. R. Jeong, M. H. Ha, S. H. Kim, and M. W. Byun (2000) Induction of nitric oxide and TNF-$\alpha$ by herbal plant extract in mouse macrophage. J. Korean Soc. Food Sci. Nutr. 29: 342-348.

23.
Ministry of Food and Drug Safety. http://www.mfds.go.kr/index. do?x=15&searchkey=title:contents&mid=1161&searchword=μ¹º½ &y=1&pageNo=1&seq=6218&cmd=v (2012).

24.
Yoon, S. B., H. S. Han, and Y. J. Lee (2011) Effect of Scutellariae radix extract on the proinflammatory mediators in RAW 264.7 cells induced by LPS. Korean J. Herbol. 26: 75-81.

25.
Lee, Y. H. and E. M. Lim (2013) Anti-inflammatory effect of Ligustri lucidi fructus water extract in RAW 264.7 cells by LPS. J. Korean Obstet. Gynecol. 26: 66-81.

26.
Ippouchi, K., H. Itou, K. Azuma, and H. Higashio (2002) Effect of naturally occurring organosulfur compounds on nitric oxide production in lipopolysaccharide-activated macrophages. Life Sci. 71: 411-419. crossref(new window)

27.
Park, C. M., J. Y. Park, K. H. Noh, J. H. Shin, and Y. S. Song (2011) Taraxacum officinale Weber extracts inhibit LPS-induced oxidative stress and nitric oxide production via the NF-${\kappa}B$ modulation in RAW 264.7 cells. J. Ethnopharmacol. 133: 834-842. crossref(new window)

28.
Lee, A. K., S. H. Sung, Y. C. Kim, and S. G. Kim (2003) Inhibition of lipopolysaccharide-inducible nitric oxide synthase, TNF-$\alpha$ and COX-2 expression by sauchinone effects on I-κBα phosphorylation, C/EBP and AP-1 activation. Br. J. Pharmacol. 139: 11-20. crossref(new window)

29.
Perez-Vizcaino, F. and J. Duarte (2010) Flavonols and cardiovascular disease. Mol. Aspects Med. 31:478-494. crossref(new window)

30.
Lee, H. A., S. J. Han, S. H. Hong, and O. J. Kim (2014) Effects of onion peel water extract on the blood lipid profiles in mice fed a high-fat diet. Korean J. Med. Crop. Sci. 22: 203-209. crossref(new window)

31.
Cho, S. Y., S. J. Park, M. J. Kwon, T. S. Jeong, S. H. Bok, W. Y. Choi, W. I. Jeong, S. Y. Ryu, S. H. Do, C. S. Lee, J. C. Song, and K. S. Jeong (2003) Quercetin suppresses proinflammatory cytokines production through MAP kinases and NF-${\kappa}B$ pathway in lipopolysaccharide- stimulated macrophage. Mol. Cell Biochem. 243: 153-160. crossref(new window)

32.
Anandakumar, S., J. A. Joseph, B. Bethapudi, A. Agarwal, and E. B. Jeong (2014) Anti-inflammatory effects of turmeric (Curcuma longa L.) extract on acute and chronic inflammation models. J. Korean Soc. Food Sci. Nutr. 43: 612-617. crossref(new window)

33.
Towbin, H., W. Pignat, and I. Wiesenberg (1995) Time-dependent cytokine production in the croton oil-induced mouse ear oedema and inhibition by prednisolone. Inflamm. Res. 44: 160-161. crossref(new window)

34.
Kim, J. H., Y. J. Park, N. S. Jo, D. K. Kim, S. H. Kim, and T. Y. Shin (2014) Acute oral toxicity of the bark of Diospyros kaki in mice. Korean J. Pharmacogn. 45: 181-185.

35.
Organizaion for economic cooperation and development (OECD) (2001) OECD guideline for the testing of chemicals revised graft guideline 420.