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Cell Cycle Arrest Effects by Artemisia annua Linné in Hep3B Liver Cancer Cell
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  • Journal title : KSBB Journal
  • Volume 30, Issue 4,  2015, pp.175-181
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2015.30.4.175
 Title & Authors
Cell Cycle Arrest Effects by Artemisia annua Linné in Hep3B Liver Cancer Cell
Kim, Eun Ji; Kim, Guen Tae; Kim, Bo Min; Lim, Eun Gyeong; Kim, Sang Yong; Ha, Sung Ho; Kim, Young Min; Yoo, Je-Geun;
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Cells proliferate via repeating process that growth and division. This process is G1, S, G2 and M four phases consists. Monitoring the progression of the cell cycle is a specific step that to be a continuous process is repeated to adjust the start of the next step. At this time, this process is called a Checkpoint. Currently, there are three known checkpoints that G1-S phase, G2-M phase, and the M phase. In this study, we confirmed that cell cycle arrest effects by ethanol extracts of Artemisia annua Linne (AAE) in Hep3B liver cancer cells. AAE was regulated proteins which involved in cell cycle such as pAkt, pMDM2, p53, p21, pCDK2 (T14/Y15). AAE induced cell cycle arrest in G1 checkpoint through phosphorylation of CDK2. Akt and p53 upstream is inhibited by AAE and p53 activated by non-activated pMDM2, p53 inhibitor. Thereby, activated p53 is transcript to p21 and activated p21 protein is combined with Cyclin E-pCDK2 complex. Therefore, we confirmed that AAE-induced cell cycle arrest was occurred by p21-Cyclin E-pCDK2 complex by inhibition of pAkt signal. Because of this cell cycle can't pass to S phase from G1 phase.
Hep3B;Cell cycle arrest;Cyclin E-Cdk2;AAE;G1 arrest;
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생명과학회지, 2016. vol.26. 6, pp.663-672 crossref(new window)
Apoptotic Effects and Cell Cycle Arrest Effects of Extracts from Cnidium monnieri (L.) Cusson through Regulating Akt/mTOR/GSK-3β Signaling Pathways in HCT116 Colon Cancer Cells, Journal of Life Science, 2016, 26, 6, 663  crossref(new windwow)
Montalto, G., M. Cervello, L. Giannitrapani, F. Dantona, A. Terranova, and L. A. M. Castagnetta (2002) Epidemiology, risk factors, and natural history of hepatocellular carcinoma. Ann. NY Acad. Sci. 963: 13-20.

Yun, H. J., S. G. Hwang, H. J. Yun, C. H. Kim, G. S. Seo, W. H. Park, and S. D. Park (2006) Anticancer effect of Rheum Rhizoma on human liver cancer HepG2 cells. Korea J. Herbol. 4: 27-36.

Ryu, J. H., S. J. Lee, M. J. Kim, J. H. Shin, S. K. Kang, K. M. Cho, and A. J. Sung (2011) Antioxidant and anticancer activities of Artemisia annua L. and determination of functional compounds. J. Korea Soc. Food Sci. Nutr. 40: 509-516. crossref(new window)

Romero, M. R., M. A. Serrano, M .Vallejo, T. Efferth, M. Alvarez, and J. J. Marin (2006) Antiviral effect of artemisinin from Artemisia annua against a model member of the Flaviviridae family, the bovine viral diarrhoea virus (BVDV). Planta Med. 72: 1169-1174. crossref(new window)

Schmid, G., and W. Hofheinz (1983) Total synthesis of qinghaosu. J. Am. Chem. Soc. 105: 624-625. crossref(new window)

Avery, M. A., W. K. M. Chong, and White C. Jennings (1992) Stereoselective total synthesis of (dextro)-artemisinin, the antimalarial constituent of Artemisia annua L. J. Am. Chem. Soc. 114: 974-979. crossref(new window)

Park, M. T. and S. J. Lee (2003) Cell cycle and cancer. J. Biochem. Mol. Biol. 36: 60-65. crossref(new window)

Novak, B., J. C. Sible, and J. J. Tyson (2003) Checkpoints in the Cell Cycle, Encyclopedia of Life Sciences. DOI:10.1038/npg.els.0 001355 crossref(new window)

Lee, M. H. and H. Y. Yang (2003) Regulators of G1 cyclin-dependent kinases and cancers. Cancer Metastasis Rev. 22: 435-449. crossref(new window)

Kang, M. S., S. M. Ju, B. J. Jeon, H. M. Yang, W. S. Kim, and B. H. Jeon (2011) Nardostachys chinensis induces G0/G1 phase cell cycle arrest in U937 cells. Korean J. Oriental Physiol. Pathol. 25: 189-194.

Giono, L. E. and J. J. Manfredi (2007) Mdm2 is required for inhibition of Cdk2 activity by p21, thereby contributing to p53-dependent cell cycle arrest. Mol. Celluar Biol. 11: 4166-4178.

Yoko, O., K. Shohei, O. Toshiyuki, I. Yuko, S. Toshiaki, T. Keiji, M. Norihisa, and G. Yukiko (2002) Akt enhances Mdm2-mediated Ubiquitination and Degradation of p53. J. Biol. Chem. 24: 21843- 21850.

Arnold, J. (1997) p53, the cellular gatekeeper for growth and division. Cell 88: 323-331. crossref(new window)

Moll, U. M. and O. Petrenko (2003) The MDM2-p53 Interaction. Mol. Cancer Res. 1: 1001-1008.

Lee, J. H., W. Y. Choi 1, Y. H. Choi 2 and B. T. Choi (2009) Cell cycle arrest by treatment of D-Ala2-Leu5-enkephalin in human Leukemia cancer U937 cell. J. Life Sci. 5: 620-624.

Arnold, J., F. Zhaohui, W. M. Tak (2014) Coordination and communication between the p53 IGF-1-Akt-TOR signal transduction pathways. Cold Spring Harbor Laboratory Press, 9.

Mirela, F., S. Chintda, E. Carol, and D. Oliver (2012) Effect of OI 3K/Akt pathway inhibition-mediated G1 arrest on chemosensitization in ovarian cancer cells. Anticancer Res. 32: 445-452.

Donnellan, R. and R. Chetty (1999) Cyclin E in human cancers. FASEB J. 13: 773-780. crossref(new window)

Pan, M. H., W. H. Chen, S. Y. Shiau, C. T. Ho, and J. K. Lin (2002) Tangeretin induces cell-cycle G1 arrest through inhibiting cyclindependent kinases 2 and 4 activities as well as elevating Cdk inhibitors p21 and p27 in human colorectal carcinoma cells. Carcinogenesis 10: 1677-1684.