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Anti-inflammatory Effect of Zostera marina Ethanolic Extract on LPS-induced RAW264.7 Cells and Mouse Model
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  • Journal title : KSBB Journal
  • Volume 30, Issue 4,  2015, pp.182-190
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2015.30.4.182
 Title & Authors
Anti-inflammatory Effect of Zostera marina Ethanolic Extract on LPS-induced RAW264.7 Cells and Mouse Model
Kim, Min-Ji; Bae, Nan-Young; Kim, Koth-Bong-Woo-Ri; Park, Ji-Hye; Park, Sun-Hee; Cho, Young-Je; Ahn, Dong-Hyun;
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 Abstract
The Zostera marina ethanolic extract (ZMEE) was tested in this study to investigate the anti-inflammatory activity in LPS-induced RAW 264.7 cells and mouse model. Nitric oxide production and inducible nitiric oxide synthase expression in cells treated with ZMEE was reduced significantly in a dose-dependent manner. Similarly, the secretion of pro-inflammatory cytokines such as interleukin (IL)-6, IL-, and TNF- was inhibited markedly. In addition, the expression of nuclear factor kappa B (NF-) and the phosphorylation of JNK, ERK, and p38 MAPKs was suppressed by ZMEE as well. In vivo test, ZMEE attenuated the croton oil-induced mouse ear edema and there were no mortalities in mice administered 5,000 mg/kg body weight of ZMEE during the observation periods. The results in photomicrograph of mice ear tissue showed the reduction of dermal thickness and the number of infiltrated mast cells. These results indicate that ZMEE inhibits the production of LPS-induced pro-inflammatory mediators, suggesting that ZMEE may be a potential material for anti-inflammatory therapies.
 Keywords
Zostera marina;Anti-inflammation;Ear edema;Nuclear factor kappa B;MAPKs;
 Language
Korean
 Cited by
1.
LPS로 유발한 대식세포의 염증반응과 마우스 귀 부종에 대한 구멍갈파래 에탄올 추출물의 항염증 효과,김민지;김민주;김꽃봉우리;박선희;최현덕;박소영;장미란;임무혁;안동현;

한국미생물생명공학회지, 2016. vol.44. 4, pp.479-487 crossref(new window)
2.
NF-κB와 MAPKs 활성 저해를 통한 미야베 모자반(Sargassum miyabei Yendo) 에탄올 추출물의 항염증 활성,김민지;배난영;김꽃봉우리;박선희;장미란;임무혁;안동현;

한국미생물생명공학회지, 2016. vol.44. 4, pp.442-451 crossref(new window)
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