JOURNAL BROWSE
Search
Advanced SearchSearch Tips
Characterization and Antimicrobial Activity against Gardnerella vaginalis of Vaginal Lactobacillus spp. Isolated from Korean Women
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
  • Journal title : KSBB Journal
  • Volume 30, Issue 5,  2015, pp.239-244
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2015.30.5.239
 Title & Authors
Characterization and Antimicrobial Activity against Gardnerella vaginalis of Vaginal Lactobacillus spp. Isolated from Korean Women
Kim, YongGyeong; Kang, Chang-Ho; Shin, YuJin; Paek, Nam-Soo; So, Jae-Seong;
  PDF(new window)
 Abstract
Bacterial vaginosis (BV) is caused by microbial imbalance of the vaginal ecosystem and overgrowth of anaerobic bacteria. The antibiotic treatment often results in very high recurrence of BV because it disturbs the vaginal ecosystem. The high recurrence rates suggest a need for alternative therapeutic methods and probiotics are being recognized as alternative or additional treatment method for BV. The purpose of this study was to investigate how human vaginal isolates of Lactobacillus spp. inhibit the BV-associated pathogen Gardnerella vaginalis. Results show that selected strains significantly reduced the viability of G. vaginalis. Among these selected strains KLB410 and KLB416 were further selected based on acid/bile tolerance and identified through 16S rRNA gene sequencing being Lactobacillus plantarum. Further studies are underway to demonstrate that the selected strain can be applied as potential probiotics for recovering vaginal ecosystem.
 Keywords
Bacterial vaginosis;Gardnerella vaginalis;Probiotics;Lactobacillus spp.;
 Language
Korean
 Cited by
 References
1.
Mastromarino, P., B. Vitali, and L. Mosca (2013) Bacterial vaginosis: A review on clinical trials with probiotics. New Microbiologica 36: 229-238.

2.
Chen, K. C., P. S. Forsyth, T. M. Buchanan, and K. K. Holmes (1979) Amine content of vaginal fluid from untreated and treated patients with nonspecific vaginitis. J. Clin. Invest. 63: 826-835.

3.
Verstraelena, H., and A. C Senok (2005) Vaginal lactobacilli, probiotics, and IVF. Reprod. Biomed. Online 11: 674-675. crossref(new window)

4.
Wiesenfeld, H. C, S. L. Hillier, M. A. Krohn, D. V. Landers, and R. L. Sweet (2003) Bacterial vaginosis is a strong predictor of Neisseria gonorrhoeae and Chlamydia trachomatis infection. Clin. Infect. Dis. 36: 663-668. crossref(new window)

5.
Bradshaw, C. S., A. N. Morton, J. Hocking, S. M. Garland, M. B. Morris, L. M. Moss, L. B. Horvath, I. Kuzevska, and C. K. Fairley (2006) High recurrence rates of bacterial vaginosis over the course of 12 months after oral metronidazole therapy and factors associated with recurrence. J. Infect. Dis. 193: 1478-1486. crossref(new window)

6.
Falagas, M. E., G. I. Betsi, and S. Athanasiou (2007) Probiotics for the treatment of women with bacterial vaginosis. Clin. Microbiol. Infect. 13: 657-664. crossref(new window)

7.
Borge, S., J. Silva, and P. Teixeira (2013) The role of lactobacilli and probiotics in maintaining vaginal health. Arch. Gynecol. Obstet. 289: 479-489.

8.
Bastani, P., A. Homayouni, S. Ziyadi, and V. G. Tabrizian (2012) Dairy Probiotic Foods and Bacterial Vaginosis: A Review on Mechanism of Action. pp. 445-456. In: Probiotics, Rigobelo, E. C. (Ed.), Intech., USA.

9.
Chang, C. E., S. I. Pavlova, L. Tao, E. Kim, S. C. Kim, H. S. Yun and J. S. So (2002) Molecular Identification of Vaginal Lactobacillus spp. Isolated from Korean Women. J. Microbiol. Biotechnol. 12: 312-317.

10.
Guo, X. H., J. M. Kim, H. M. Nam, S. Y. Park and J. M. Kim (2010) Screening lactic acid bacteria from swine origins for multistrain probiotics based on in vitro functional properties. Anaerobe 16: 321-326. crossref(new window)

11.
Kassa, I. A., M. Hamze, D. Hober, N. E. Chihib and D. Drider (2014) Identification of Vaginal Lactobacilli with Potential Probiotic Propertied Isolated from Women in North Lebanon. Microb. Ecol. 67: 722-734. crossref(new window)

12.
Borchardt, K. A., B. S. Adly, R. F. Smith, J. Eapen, and C. B. Beal (1989) Importance of Gardnerella vaginalis as an aetiological agent in bacterial vaginosis. Genitourin. Med. 65: 285.

13.
Machado, A., K. K. Jefferson, and N. Cerca (2013) Interactions between Lactobacillus crispatus and Bacterial Vaginosis (BV)-associated bacterial species in initial attachment and biofilm formation. Int. J. Mol. Sci. 14: 12004-12012. crossref(new window)

14.
Dunne, C., L. O'Mahony, L. Murphy, G. Thornton, D. Morrissey, S. O'Halloran, M. Feeney, S. Flynn, G. Fitzgerald, C. Daly, B. Kiely, G. C. O'Sullivan, F. Shanahan, and J K. Collins (2001) In vitro selection criteria for probiotic bacteria of human origin: Correlation with in vivo findings. Am. J. Clin. Nutr. 73: 386S-392S. crossref(new window)

15.
Tasi, C. C., H. Y. Hsih, H. H. Chiu, Y. Y. Lai, J. H. Liu, B. Yu (2005) Antagonistic activity against Salmonella infection in vitro and in vivo for two Lactobacillus strains from swine and poultry. Int. J. Food Microbiol. 102: 185-194. crossref(new window)

16.
Mishra, V., and D. N. Prasad (2005) Application of in vitro methods for selection of Lactobacillus casei strains as potential probiotics. Int. J. Food Microbiol. 103: 109-115. crossref(new window)

17.
Gilliland, S. E., T. E. Staley, and L. J. Bush (1984) Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct. J. Dairy Sci. 67: 3045-5301. crossref(new window)

18.
Lin, W. H., C. F. Hwangb, L. W. Chenc, and H. Y. Tsen (2006) Viable counts, characteristic evaluation for commercial lactic acid bacteria products. Food Microbiol. 23: 74-81. crossref(new window)

19.
Del, R. B. Sgorbati, M. Moglioli and D. Palenzona (2000) Adhesion, autoaggregation and hydrophobicity of 13 strains of bafidobacterium longum. Lett. Appl. Microbiol. 31:438-442. crossref(new window)

20.
Kos, B., J. Suskoviae, S. Vukoviae, M. Simpraga, J. Frece and S. Matosiae (2003) Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J. Appl. Microbiol. 94: 981-987. crossref(new window)

21.
Malik, S., M. I. Petrova, I. J. J. Claes, T. L. A. Verhoeven, P. Busschaert, M. Vaneechoutte, B. Lievens, I. Lambrichts, R. J. Siezen, J. Balzarini, J. Vanderleyden and S. Lebeer (2013) The highly autoaggregative and adhesive phenotype of the vaginal Lactobacillus plantarum strain CMPG5300 is sortase dependent. Appl. Environ. Microbiol. 79: 4576-4585. crossref(new window)

22.
Kleerebezem, M., J. Boekhorst, R. van Kranenburg, D. Molenaar, O. P. Kuipers, R. Leer, R. Tarchini, S. A. Peters, H. M. Sandbrink, M. W. E. J. Fiers, W. Stiekema, R. M. K. Lankhorst, P. A. Bron, S. M. Hoffer, M. N. N. Groot, R. Kerkhoven, M. de Vries, B. Ursing, W. M. de Vos, and R. J. Siezen (2003) Complete genome sequence of Lactobacillus plantarum WCFS1. Proc. Natl. Acad. Sci. USA 100: 1990-1995. crossref(new window)

23.
Ministry of Food and Drug Safety, The standard for health functional food. http://www.mfds.go.kr. (2014).