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Antioxidant Activity of the Halophyte Ligustrum japonicum
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  • Journal title : KSBB Journal
  • Volume 30, Issue 6,  2015, pp.275-282
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2015.30.6.275
 Title & Authors
Antioxidant Activity of the Halophyte Ligustrum japonicum
Baek, Seung Oh; Kim, Hojun; Jeong, Heejeong; Ju, Eunsin; Kong, Chang-Suk; Seo, Youngwan;
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 Abstract
Dried samples of Ligustrum japonicum were extracted twice: with methylene chloride and with methanol (MeOH), respectively. The combined crude extracts were successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by liquid-liquid partition. Antioxidant activities of crude extract and its solvent fractions were evaluated by measuring authentic , and generated from 3-morpholinsydnonimine (SIN-1) as well as degree of occurrence of intracellular ROS in HT 1080 cells, and genomic DNA oxidation. The 85% aq.MeOH and n-BuOH fractions exhibited the good antioxidant activity. Further purification of the 85% aq.MeOH fracition led to the isolation of Oleanolic acid (1), Maslinic acid (2), and Ursolic acid (3). All compounds showed the significant antioxidant effects in all assay systems.
 Keywords
Ligustrum japonicum;Antioxidant;Reactive oxygen species (ROS);Peroxynitrite;
 Language
Korean
 Cited by
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