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Optimization of Cellulase Production from Paenibacillus jamilae BRC 15-1
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  • Journal title : KSBB Journal
  • Volume 30, Issue 6,  2015, pp.283-290
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2015.30.6.283
 Title & Authors
Optimization of Cellulase Production from Paenibacillus jamilae BRC 15-1
Cha, Young-Lok; Yoon, Young-Mi; Yoon, Ha-Yan; Kim, Jung Kon; Yang, Ji-Young; Na, Han-Beur; Ahn, Jong-Woong; Moon, Youn-Ho; Choi, In-Hu; Yu, Gyeong-Dan; Lee, Ji-Eun; An, Gi Hong; Lee, Kyeong-Bo;
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In this study was selected the cellulolytic microorganism and investigated optimum condition of cellulase production for the cellulosic bioethanol production. A bacterial strain Paenibacillus jamilae BRC15-1, was isolated from soil of domestic reclaimed land. For optimizing cellulase production from the selected strain, various culture parameters were investigated such as culture medium, pH (pH 4~10), temperature () and culture time (2~72 h). As a result, P. jamilae BRC15-1 efficiently produced cellulase from cellulosic biomass under following conditions: 24 h of culture time (pH 7, ) in manufactured media of CMC (carboxymethyl cellulose) with peptone. Optimum saccharifying condition of crude enzyme produced from P. jamilae BRC15-1 was identified on pH 6 and of reaction temperature, respectively. This crude enzyme from P. jamilae BRC15-1 was used for saccharification of pretreated sweet sorghum (Sorghum bicolor var. dulciusculum Ohwi) bagasse under the optimal condition. Finally, pretreated sweet sorghum bagasse including 0.1 g of glucan was saccharified by crude enzyme of P. jamilae BRC15-1 into 2.75 mg glucose, 0.79 mg xylose and 1.12 mg arabinose.
Cellulase;Bioethanol;Saccharification;Paenibacillus sp.;Sweet sorghum bagasse;
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