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Characterization of α-agarase from Alteromonas sp. SH-1
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  • Journal title : KSBB Journal
  • Volume 31, Issue 2,  2016, pp.113-119
  • Publisher : Korean Society for Biotechnology and Bioengineering
  • DOI : 10.7841/ksbbj.2016.31.2.113
 Title & Authors
Characterization of α-agarase from Alteromonas sp. SH-1
Lee, Sol-Ji; Shin, Da-Young; Kim, Jae-Deog; Lee, Dong-Geun; Lee, Sang-Hyeon;
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A novel agar-degrading marine bacterium, SH-1 strain, was isolated from seashore of Namhae at Gyeongnam province, Korea. The SH-1 strain exhibited 98% similarity with Alteromonas species based on 16S rDNA sequencing and named as Alteromonas sp. SH-1. Alteromonas sp. SH-1 showed agarase activity of 348.3 U/L (1.67 U/mg protein). The molecular masses of the enzymes were predicted as about 85 kDa and 110 kDa by SDS-PAGE and zymogram. The enzymatic activity was optimal at and the relative agarase activity was decreased as temperature increase from and thus about 90% and 70% activities were shown at and , respectively. The optimum pH was 6.0 for agarase activity in 20 mM Tris-HCl buffer and activities were less than 70% and 85% activity at pH 5.0 and pH 7.0, respectively, compared with that at pH 6. Agarase activity has remained over 90% at after 1.5 hour exposure at this temperature. However, its activity was less than 60% at after 0.5 h exposure at this temperature. The enzymes produced agarooligosaccharides such as agaropentaose and agarotriose from agarose, indicating that the agarases are -agarases. Thus, Alteromonas sp. SH-1 and its agarases would be useful for the industrial production of agarooligosaccharides which are known as having anticancer and antioxidation activities.
-Agarase;agaropentaose;agarooligosaccharide;Alteromonas sp. SH-1;marine derived bacterium;
 Cited by
Jang, H. J., D. G. Lee, S. W. Lee, M. J. Jeon, W. J. Chun, K. K. Kwon, H. S. Lee, and S. H. Lee (2011) Isolation of a marinederived Flammeovirga sp. mbrc-1 strain and characterization of its agarase. KSBB J. 26: 552-556. crossref(new window)

Lee, D. G., N. Y. Kim, M. K. Jang, O. H. Lee, and S. H. Lee (2007) Isolation and characterization of a marine bacterium Thalassomonas sp. SL-5 producing ${\beta}$-agarase. J. Life Sci. 17: 70-75. crossref(new window)

Hong, J. H., J. J. Lee, S. H. Hur, H. S. Choi, and J. Y. Kong (2001) Effect of agarooligosaccharides on the growth of intestinal bacteria. J. Fd. Hyg. Safety 16: 11-15.

Seo, Y. B., Y. Lu, W. J. Chi, H. R. Pack, K. J. Jeong, S. K. Hong, and Y. K. Chang (2014) Heterologous expression of a newly screened ${\beta}$-agarase from Alteromonas sp. GNUM1 in Escherichia coli and its application for agarose degradation. Process Biochem. 49: 430-436. crossref(new window)

Yang, M., X. Mao, N. Liu, Y. Qiu, and C. Xue (2014) Purification and characterization of two agarases from Agarivorans albus OAY 02. Process Biochem. 49: 905-912. crossref(new window)

Hassairi, I., R. Ben Amar, M. Nonus, and B. B. Gupta (2001) Production and separation of ${\alpha}$-agarase form Alteromonas agarlyticus strain GJ1B. Biores. Technol. 79: 47-51. crossref(new window)

Kim, Y. J. (2010) Properties of the agarase and its gene isolated from a marine bacterium, Tamlana agarivorans. M.S. dissertation, University of Chungnam, Taejon, Korea.

Kim, Y. N. (2011) Isolation and purification of new agarase from marine bacterium. M.S. dissertation, University of Kyungsang, Jinju, Korea.

Lee, D. G., M. K. Jang, O. H. Lee, N. Y. Kim, S. A. Ju, and S. H. Lee (2008) Over-production of a glycoside hydrolase family 50 ${\beta}$-agarase from Agarivorans sp. JA-1 in Bacillus subtilis and the whitening effect of its product. Biotechnol. Lett. 30: 911-918. crossref(new window)

Kim, H. T., S. Lee, D. Lee, H. S. Kim, W. G. Bang, K. H. Kim, and I. G. Choi (2010) Overexpression and molecular characterization of Aga50D from Saccharophagus degradans 2-40: An exo-type ${\beta}$-agarase producing neoagarobiose. Appl. Microbiol. Biotechnol. 86: 227-234. crossref(new window)

Lakshmikanth, M., S. Manohar, Y. Souche, and J. Lalitha (2009) Extracellular ${\beta}$-agarase LSL-1 producing neoagarobiose from a newly isolated agar-liquefying soil bacterium, Acinetobacter sp., AG LSL-1. Process Biochem. 44: 999-1003. crossref(new window)

Yang, J. I., L. C. Chen, Y. Y. Shih, C. Hsieh, C. Y. Chen, W. M. Chen, and C. C. Chen (2011) Cloning and characterization of ${\beta}$-agarase AgaYT from Flammeovirga yaeyamensis strain YT. J. Biosci. Bioeng. 112: 225-232. crossref(new window)

Temuujin, U., W. J. Chi, Y. K. Chang, and S. K. Hong (2012) Identification and biochemical characterization of Sco3487 from Streptomyces coelicolor A3(2), an exo- and endo-type ${\beta}$-agarase-producing neoagarobiose. J. Bacteriol. 194: 142-149. crossref(new window)

Lee, D. G., O. H. Lee, H. J. Jang, M. K. Jang, K. H. Yoo, and S. H. Lee (2008) Isolation and characterization of a marine derived bacterium Glaciecola sp. SL-12 producing ${\beta}$-agarase. J. Life Sci. 18: 58-62. crossref(new window)

Jang, M. K., S. W. Lee, D. G. Lee, N. Y. Kim, K. H. Yu, H. J. Jang, S. Kim, A. Kim, and S. H. Lee (2010) Enhancement of the thermostability of a recombinant ${\beta}$-agarase, AgaB, from Zobellia galactanivorans by random mutagenesis. Biotechnol. Lett. 32: 943-949. crossref(new window)

Chi, W. J., J. H. Lim, D. Y. Park, M. C. Kim, C. J. Kim, Y. K. Chang, and S. K. Hong (2013) Isolation and characterization of a novel agar degrading bacterium Alteromonas macleodii subsp. Gnum08120, from red macroalgae. Korean J. Microbiol. Biotechnol. 41: 8-16. crossref(new window)

Kim. J. H., and S. K. Hong (2012) Isolation and characterization of an agarase-producing bacterial strain, Alteromonas sp. GNUM-1, from the west Sea, Korea. J. Microbiol. Biotech. 22: 1621-1628. crossref(new window)

Leon, O., L. Quintana, G. Peruzzo and J. C. Slebe (1992) Purification and properties of an extracellular agarase from Alteromonas sp. strain C-1. Microbiol. 58: 4060-4063.

Wang, J., H. Mou, X. Jiang, and H. Guan (2006) Characterization of a novel ${\beta}$-agarase from marine Alteromonas sp. SY37-12 and its degrading products. Appl. Microbiol. Biotechnol. 71: 833-839. crossref(new window)

Ohta, Y., Y. Hatada, M. Miyazaki, Y. Nogi, S. Ito, and K. Horikoshi (2005) Purification and characterization of a novel from a Thalassomonas sp. Curr. Microbiol. 50: 212-216. crossref(new window)

Fu, X. T. and S. M. Kim (2010) Agarase: Review of major sources, categories, purification method, enzyme characteristics and applications. Mar. Drugs 8: 200-218. crossref(new window)

Kim, J. H., Y. H. Kim., S. K. Kim, B. W. Kim, and S. W. Nam (2011) Properties and Industrial applications of seaweed polysaccharides-degrading enzymes from the marine microorganisms. Korean J. Microbiol. Biotechnol. 39: 189-199.

Kohtaro, K., M. Masuda, Y. Iwasaki, H. Nakagawa, R. Kobayashi, and S. Usami (1999) Purification and characterization of a novel ${\beta}$-agarase from an alkalophilic bacterium, Alteromonas sp. E-l. J. Biosci. Bioengi. 87: 436-441. crossref(new window)