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Anti-Inflammatory Effect of Alginate Oligosaccharides Produced by an Alginate-Degrading Enzyme from Shewanella oneidensis PKA1008 on LPS-Induced RAW 264.7 Cells
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 Title & Authors
Anti-Inflammatory Effect of Alginate Oligosaccharides Produced by an Alginate-Degrading Enzyme from Shewanella oneidensis PKA1008 on LPS-Induced RAW 264.7 Cells
Kim, Min-Ji; Bae, Nan-Yong; Bark, Si-Woo; Kim, Koth-Bong-Woo-Ri; Park, Ji-Hye; Park, Sun-Hee; Ahn, Dong-Hyun;
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 Abstract
The anti-inflammatory effect of alginate oligosaccharides on LPS-induced RAW 264.7 cells was investigated at different time points (0-60 h). The alginate oligosaccharides were produced by an alginate-degrading enzyme from Shewanella oneidensis PKA1008. The alginate oligosaccharides decreased the production of nitric oxide and proinflammatory cytokines [tumor necrosis factor-, interleukin (IL)-, and IL-6] in a dose-dependent manner. The alginate oligosaccharides showed peak anti-inflammatory activity after 36 h of incubation; at that time point, reduced protein expression of NF- p65, iNOS, and COX-2 was detected. Furthermore, the alginate oligosaccharide treatment reduced the formation of ear edema at 36 h compared to samples examined at 0 h when the oligosaccharides were administered at 50 and 250 mg/kg body weight, as well as dermal thickness and mast cell numbers in a histological analysis. These results suggest that alginate oligosaccharides are a promising anti-inflammatory agent.
 Keywords
Alginate oligosaccharides;Anti-inflammation;Ear edema;NF-kappaB;
 Language
Korean
 Cited by
 References
1.
Bark SW, Kim KBWR, Kim MJ, Kang BK, Pak WM, Ahn NK, Choi YU, Park JH, Bae NY, Lim SM and Ahn DH. 2015. Antioxidant effect of enzymatic hydrolysate from Sargassum thunbergii using Vibrio crassostreae PKA 1002 crude enzyme. Microbiol Biotechnol Lett 43, 105-111. http://dx.doi.org/10.4014/mbl.1501.01003.

2.
Cho HJ, Shim JH, So HS and Yoon JH. 2012. Mechanism underlying the anti-inflammatory action of piceatannol induced by lipopolysaccharide. J Korean Soc Food Sci Nutr 41, 1226-1234. http://dx.doi.org/10.3746/jkfn.2012.41.9.1226. crossref(new window)

3.
Cho KJ, Lee YS and Ryu BH. 1990. Antitumor effect and immunology activity of seaweeds toward sarcoma-180. Bull Kor Fish Soc 23, 345-352.

4.
Cho M, Kim BY and Rhim JH. 2003. Degradation of alginate solution by using ${\gamma}$-irradiation and orgainc acid. Korean J Food Sci Technol 35, 67-71.

5.
Chun KS and Surh YJ. 2004. Signal transduction pathways regulating cyclooxygenase-2 expression: potential molecular targets for chemoprevention. Biochem Pharmacol 15, 1089-1100. http://dx.doi.org/10.1016/j.bcp.2004.05.031. crossref(new window)

6.
Dinarello CA, Endres S, Meydani SN, Meydani M and Hellerstein MK. 1990. Interleukin-1, anorexia, and dietary fatty acids. Ann N Y Acad Sci 587, 332-338. crossref(new window)

7.
Guha M and Mackman N. 2001. LPS induction of gene expression in human monocytes. Cell Signal 13, 85-94. http://dx.doi.org/10.1016/S0898-6568(00)00149-2. crossref(new window)

8.
Higuchi M, Hisgahi N, Taki H and Osawa T. 1990. Cytolytic mechanism of activated macrophages. Tumor necrosis factor and L-arginine dependent mechanisms act synergistically as the major cytolytic mechanism of activated macrophages. J Immunol 144, 1425-1432.

9.
Hippeli S and Elastner EF. 1999. Inhibition of biochemical model reactions for inflammatory processes by plant extracts; a review on recent developments. Free Radic Res 31, 81-87. http://dx.doi.org/10.1080/10715769900301361. crossref(new window)

10.
Horn SJ, Moen E and Ostgard K. 1999. Direct determination alginate content in brown algae by near infra-red (NIR) spectroscopy. J Appl Phycol 11, 9-13. http://dx.doi.org/10.1023/A:1008024009954. crossref(new window)

11.
Iwamoto Y, XU X, Tamura T, ODA T and Muramatsu T. 2003. Enzymatically depolymerized alginate oligomers that cause cytotoxic cytokine production in human mononuclear cells. Biosci Biotechnol Biochem 67, 258-263. http://dx.doi.org/258-263. 10.1271/bbb.67.258. crossref(new window)

12.
Jeong DH, Kang BK, Kim KBWR, Kim MJ and Ahn DH. 2014. Anti-inflammatory activity of Sargassum micracanthum water extract. J Appl Biol Chem 57, 227-234. http://dx.doi.org/10.3839/jabc.2014.036. crossref(new window)

13.
Jeong HR, Sung MS, Kim YH, Ham HM, Choi YM and Lee JS. 2012. Anti inflammatory activity of Salvia plebeia R. Br. leaf through Heme Oxygenase-${\mu}$induction in LPS-stimulated RAW 264.7 macrophages. J Korean Soc Food Sci Nutr 41, 888-894. http://dx.doi.org/10.3746/jkfn.2012.41.7.888. crossref(new window)

14.
Kang BK, Kim KBWR, Kim MJ, Bark SW, Pak WM, Kim BR, Ahn NK, Choi YU and Ahn DH. 2014. Anti-inflammatory activity of an ethanol extract of Laminaria japonica root on lipopolysaccharide-induced inflammatory responses in RAW 264.7 cells. Korean J Food Sci Technol 46, 729-733. crossref(new window)

15.
Kim DW, Chi YS, Son KH, Chang HW, Kim JS, Kang SS and Kim HP. 2002. Effects of sophoraflavanone G, a prenylated flavonoid from Sophora flavescens, on cyclooxygenase-2 and in vivo inflammatory response. Arch Pharm Res 25, 329-335. http://dx.doi.org/10.1007/BF02976635. crossref(new window)

16.
Kim JH, Kim YH, Kim SK, Kim BW and Nam SW. 2011. Properties and industrial applications of seaweed polysaccharides-degrading enzymes from the marine microorganisms. Korean J Microbiol Biotechnol 39, 189-199.

17.
Kim OJ, Lee DG, Lee SM, Lee SJ, Do HJ, Park HJ, Kim A, Lee JH and Ha MJ. 2010. Isolation and characteristics of alginate-degrading Methylobacterium sp. HJM27. Korean J Microbiol Biotechnol 38, 144-150.

18.
Kim YS, Lee SJ, Hwang JW, Kim EH, Park PJ and Jeong JH. 2012. Anti-inflammatory effects of extracts from Ligustrum ovalifolium H. leaves on RAW 264.7 macrophages. J Korean Soc Food Sci Nutr 41, 1205-1210. http://dx.doi.org/10.3746/jkfn.2012.41.9.1205. crossref(new window)

19.
Laemmli UK. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685. http://dx.doi.org/10.1038/227680a0. crossref(new window)

20.
Lee HJ, Lee CW, Choi MS, Son DJ and Hong JT. 2006. Effects of esthetic essential oils on LPS-induced nitric oxide generation in murin macrophage RAW 264.7 cells. J Soc Cosmet Sci Korea 32, 111-116.

21.
Lee HS, Choi MS, Lee YK, Park SH and Kim YJ. 1996. A study on the development of high-fiber supplements for the diabetic patients- Effect of seaweed supplementation on the lipid and glucose metabolism in streptozotocin induced diabetic rats. J Nutr Health 29, 296-306.

22.
Lee JK, Choi HS, Yoon SK and Kim WJ. 1993. Effect of extraction temperature on some quality of sea tangle extract. J Korean Soc Food Nutr 22, 771-776.

23.
Lee SR, Cho HO and Park SK. 1975. Extraction yield and quality attributes of agar from domestic seaweeds according to various pretreatment. Korean J Food Sci Technol 7, 109-114.

24.
Lee ST, Jeong YR, Ha MH, Kim SH, Byun MW and Jo SK. 2000. Induction of nitric oxide and TNF-${\alpha}$ by herbal plant extracts in mouse macrophages. J Korean Soc Food Sci Nutr 29, 342-348.

25.
Lee YS, Kim DS, Ryu BH and Lee SH. 1992. Antitumor and immunomodulating effects of seaweeds toward sarcoma-180 cell. J Kor Soc Food Nutr 21, 544-550.

26.
Liu M, Li J, Kong F, Lin J and Gao Y. 1998. Induction of immunomodulating cytokines by a new polysaccharide-peptide complex from culture mycelia of Lentinus edodes. Immunopharmacology 40, 187-198. crossref(new window)

27.
Moen E, Larsen B, Ostgard K and Jensen A. 1999. Alginate stability during high salt preservation of Ascohyllum nodosum. J Appl Phycol 11, 21-25. http://dx.doi.org/10.1023/A:1008077412543. crossref(new window)

28.
Park YM, Won JH, Yun KJ, Ryu JH, Han YN, Choi SK and Lee KT. 2006. Preventive effect of Ginkgo biloba extract (GBB) on the lipopolysaccharide-induced expressions of inducible nitric oxide synthase and cyclooxygenase-2 via suppression of nuclear factor-${\kappa}B$ in RAW 264.7 cells. Biol Pharm Bull 29, 985-990. http://dx.doi.org/10.1248/bpb.29.985. crossref(new window)

29.
Saraiva RA, Araruna MK, Oliveira RC, Menezes KD, Leite GO, Kerntopf MR, Costa JG, Rocha JB, Tome AR, Campos AR and Menezes IR. 2011. Topical anti-inflammatory effect of Caryocar coriaceum Wittm. (Caryocaraceae) fruit pulp fixed oil on mice ear edema induced by different irritant agents. J Ethnopharmacol 136, 504-510. http://dx.doi.org/10.1016/j.jep.2010.07.002. crossref(new window)

30.
Scot M, Colin GM, David J, Mills L and Brian JB. 1987. Estimation of meiobenthic nematode diversity by non specialists. Mar Pollut Bull 18, 646-649. http://dx.doi.org/10.1016/0025-326X(87)90398-5. crossref(new window)

31.
Sheeba MS and Asha VV. 2009. Cardiospermum halicacabum ethanol extract inhibits LPS induced COX-2, TNF-${\alpha}$ and iNOS expression, which is mediated by NF-${\kappa}B$ regulation, in RAW264.7 cells. J Ethnopharmacol 124, 39-44. http://dx.doi.org/10.1016/j.jep.2009.04.020. crossref(new window)

32.
Song EJ, Kim AR, Kim MJ, Lee SY, Kim KBWR, Park JG, Kim JH, Lee JW, Byun MW and Ahn DH. 2008. Effect of gamma irradiation on degrading and antioxidation activity of alginate and carrageenan. Food Eng Prog 12, 58-64. http://dx.doi.org/10.3746/jkfn.2008.37.3.357.

33.
Stankiewicz W, Dabrowski MP, Chcialowski A and Plusa T. 2002. Cellular and cytokine immunoregulation in patients with chronic obstructive pulmonary disease and bronchial asthma. Mediators Inflamm 11, 307-312. crossref(new window)

34.
Sunwoo C, Kim KBWR, Kim DH, Jung SA, Kim HJ, Jeong DH, Jung HY, Kang BK, Bark SW, Lim SM, Hong YK and Ahn DH. 2013. Optimization of conditions for the production and properties of alginate-degrading crude enzyme from Shewanella oneidensis PKA 1008. Korean J Microbiol Biotechnol 41, 372-378. http://dx.doi.org/10.4014/kjmb.1212.12002 crossref(new window)

35.
Tak PP and Firestein GS. 2001. NF-kappaB: a key role in inflammatory diseases. J Clin Invest 107, 7-11. http://dx.doi.org/10.1172/JCI11830. crossref(new window)

36.
Towbin H, Pignat W and Wiesenberg I. 1995. Time-dependent cytokine production in the croton oil-induced mouse ear oedema and inhibition by prednisolone. Inflamm Res 44, S160-161. http://dx.doi.org/10.1007/BF01778311. crossref(new window)

37.
Towbin H, Staehelin T and Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76, 4350-4354. crossref(new window)

38.
Uo MH, Joo DS, Cho SY and Min TS. 2006. Purification and characterization of the extracellular alginase produced by Bacillus licheniformis AL-577. J Korean Soc Food Sci Nutr 53, 246-252. http://dx.doi.org/10.3746/jkfn.2006.35.2.231.

39.
Yang EJ, Kim JG, Kim JY, Kim SC, Lee NH and Hyun CG. 2010. Anti-inflammatory effect of chitosan oligosaccharides in RAW 264.7 cells. Cent Eur J Biol 5, 95-102. http://dx.doi.org/10.2478/s11535-009-0066-5.

40.
Yoon HJ, Moon ME, Park HS, Im SY and Kim YH. 2007. Chitosan oligosaccharide (COS) inhibits LPS-induced inflammatory effects in RAW 264.7 macrophage cells. Biochem Biophys Res Commun. 358, 954-959. http://dx.doi.org/10.1016/j.bbrc.2007.05.042. crossref(new window)

41.
Zamora R, Vodovotz Y and Billiar TR. 2000. Inducible nitric oxide synthase and inflammatory diseases. Mol Med 6, 347-373.

42.
Zvyagintseva TN, Shevchenko NM, Nazarenko EL, Gorbach VI, Urvantseva AM, Kiseleva MI, and Isakov VV. 2005. Water-soluble polysaccharides of some brown algae of the Russian Far-East. Structure and biological action of lowmolecular mass polyuronans. J Exp Mar Biol Ecol 320, 123-131. http://dx.doi.org/10.1016/j.jembe.2004.12.027. crossref(new window)