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Effect of Heat Treatment on Male specific Coliphage and Norovirus Concentrations in Norovirus Contaminated Oyster Crassostrea gigas
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 Title & Authors
Effect of Heat Treatment on Male specific Coliphage and Norovirus Concentrations in Norovirus Contaminated Oyster Crassostrea gigas
Park, Kunbawui; Park, Yong Su; Kwon, Ji Young; Yu, Hong Sik; Lee, Hee Jung; Kim, Ji Hoe; Lee, Tae Seek; Kim, Poong Ho;
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 Abstract
Noroviruses (NoV) are known to cause acute epidemic gastroenteritis worldwide. Outbreak strains are predominantly genogroup I (GI) and genogroup II (GII) in oysters Crassostrea gigas. We investigated the changes in concentration of male specific coliphage (MSC) and NoV under heat treatment of the naturally contaminated oyster, Crassostrea gigas. After heat treatment for 5 min in , no viable MSC was detected. The concentrations of GI and GII NoV decreased by 1.65 log and 2.25 log, respectively, following heat treatment for 5 min at . Moreover, both GI and GII NoV were completely deactivated by heat treatment for 10 min at . Therefore, in order to reduce the risk of norovirus infection from contaminated oysters, immersion in boiling water for at least 10 min is recommended.
 Keywords
Heat-treatment;Male specific coliphage;Norovirus;Oyster;
 Language
Korean
 Cited by
 References
1.
Arcangeli G, Terregino C, De Benedictis P, Zecchin B, Manfrin A, Rossetti E, Magnabosco C, Mancin M and Brutti A. 2012. Effect of high hydrostatic pressure on murine norovirus in Manila clams. Lett Appl Microbiol 54, 325-329. http://dx.doi.org/10.1111/j.1472-765X.2012.03211.x. crossref(new window)

2.
Alfano-Sobsey E, Sweat D, Hall A, Breedlove F, Rodriguez R, Greene S, Pierce A, Sobsey M, Davies M and Ledford SL. 2012. Norovirus outbreak associated with undercooked oysters and secondary household transmission. Epidemiol Infect 140, 276-282. http://dx.doi.org/10.1017/S0950268811000665. crossref(new window)

3.
Blackburn, BG, Craun GF, Yoder JS, Hill V, Calderon RL, Chen N, Lee SH, Levy DA and Beach MJ. 2004. Surveillance for waterborne-disease outbreaks associated with drinking water-United States, 2001-2002. MMWR Surveill Summ 53, 23-45.

4.
Burkhardt W 3rd, Watkins WD and Rippey SR. 1992. Survival and replication of male-specific bacteriophages in molluscan shellfish. Appl Environ Microbiol 58, 1371-1373.

5.
Cabelli VJ. 1988. Microbial indicator levels in shellfish, water, and sediments from the upper Narragansett Bay conditional shellfish-growing area. Report to the Narragansett Bay Project. Narragansett Bay Project, Providence R.I.

6.
Croci L, Ciccozzi M, De Medici D, Di Pasquale S, Fiore A, Mele A and Toti L. 1999. Inactivation of hepatitis A virus in heat-treated mussels. J Appl Microbiol 87, 884-888. crossref(new window)

7.
Hewitt J and Greening GE. 2006. Effect of heat treatment on hepatitis A virus and norovirus in New Zealand greenshell mussels (Perna canaliculus) by quantitative real-time reverse transcription PCR and cell culture. J Food Prot 69, 2217-2223.

8.
Iritani N, Kaida A, Abe N, Kubo H, Sekiguchi JI, Yamamoto SP, Goto K, Tanaka T and Noda M. 2014. Detection and genetic characterization of human enteric viruses in oysterassociated gastroenteritis outbreaks between 2001 and 2012 in Osaka City, Japan. J Med Virol 86, 2019-2025. http://dx.doi.org/10.1002/jmv.23883. crossref(new window)

9.
Jothikumar N, Lowther JA, Henshilwood K, Lees DN, Hill VR and Vinje J. 2005. Rapid and sensitive detection of noro-viruses by using TaqMan-based one-step reverse transcription-PCR assays and application to naturally contaminated shellfish samples. Appl Environ Microbiol 71, 1870-1875. crossref(new window)

10.
Koopmans M, Bonsdor CH, Vinje J, Medici D and Monroe S. 2002. Foodborne viruses. FEMS Microbiol 26, 187-205. crossref(new window)

11.
Kwon JY, Park K, Song KC, Lee HJ, Park JH, Kim JD and Son KT. 2007. Evaluation of the bacteriological quality of a shellfish-growing area in Kamak bay, Korea. Korean J Fish Aquat Sci 11, 7-14. http://dx.doi.org/10.5657/KFAS.2007.0007.

12.
Lowther JA, Gustar NE, Powell AL, Hartnell RE and Lees DN. 2012. Two-year systematic study to assess norovirus contamination in oysters from commercial harvesting area in the United Kingdom. Appl Environ Microbiol 78, 5812-5817. crossref(new window)

13.
Moon A, Hwang IG and Choi WS. 2011. Prevalence of noroviruses in oyster in Korea. Food Sci Biotechnol 20, 1151-1154. crossref(new window)

14.
Park K, Jo MR, Lee HJ, Kwon JY, Son KT and Lee TS. 2011. Evaluation of the effect of the dischatged water from Bong stream after events on the bacteriological water quality in Gangjinman, korea. Korean J Fish Aquat Sci 44, 622-629. http://dx.doi.org/10.5657/KFAS.2011.0622. crossref(new window)

15.
Park K, Jo MR, Kim YK, Lee HJ, Kwon JY, Son KT and Lee TS. 2012. Evaluation of the effects of the inland pollution sources after rainfall events on the bacteriological water quality in Narodo area, korea. Korean J Fish Aquat Sci 45, 414-422. http://dx.doi.org/10.5657/KFAS.2012.0414. crossref(new window)

16.
Shin SB, Oh EG, Yu HS, Son KT, Lee HJ, Park JY and Kim JH. 2013. Genetic diversity of noroviruses detected in Oyster in Jinhae bay, Korea. Food Sci Biotechnol 22, 1453-1460. http://dx.doi.org/10.1007/s10068-013-0237-z.

17.
Shin SB, Oh EG, Lee HJ, Kim YK, Lee TS and Kim JH. 2014. Norovirus quantification in oysters Crassostrea gigas Collected from Tongyeoung, Korea. Korean J Fish Aquat Sci 47, 501-507. http://dx.doi.org/10.5657/KFAS.2014.0501. crossref(new window)

18.
Simmons G, Garbutt C, Hewitt J and Greening G. 2007. A New Zealand outbreak of norovirus gastroenteritis linked to the consumption of imported raw Korean oysters. N Z Med J 120, U2773.

19.
Sow H, Desbiens M, Morales-Rayas R, Nqazoa SE and Jean J. 2011. Heat inactivation of hepatitis A virus and a norovirus surrogate in soft-shell clams (Mya arenaria). Foodborne Pathog Dis 8, 387-393. http://dx.doi.org/10.1089/fpd.2010.0681. crossref(new window)

20.
Ueki Y, Shoji M, Suto A, Tanabe T, Okimura Y, Kikuchi Y, Saito N, Sano D and Omura T. 2007. Persistence of caliciviruses in artificially contaminated oysters during depuration. Appl Environ Microbiol 73, 5698-5701. crossref(new window)

21.
Webby RJ, Carville KS, Kirk MD, Greening G, Ratcliff RM, Crerar SK, Dempsey K, Sarna M, Stafford R, Patel M and Hall G. 2007. Internationally distributed frozen oyster meat causing multiple outbreaks of norovirus infection in Australia. Clin Infect Dis 44, 1026-1031. crossref(new window)