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Defecation of Norovirus from the Oyster Crassostrea gigas by Depuration Following Translocation of the Growing Area
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 Title & Authors
Defecation of Norovirus from the Oyster Crassostrea gigas by Depuration Following Translocation of the Growing Area
Yu, Hongsik; Park, Yong Su; An, Sera; Park, Kunbawui; Shim, Kil Bo; Song, Ki Cheol; Lee, Tae Seek;
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The efficacy of depuration following growing area translocation for the defecation of norovirus was evaluated under experimental conditions using oysters Crassostrea gigas previously subjected to bioaccumulation of this virus at a waste treatment plant discharge site. Three trials were assayed in an open experimental system with a commercial oyster farm located in a shellfish growing area approved by the Korean Shellfish Sanitation Program. Real-time reverse-transcription polymerase chain reaction (RT-PCR) was used to quantify viruses in the digestive glands of oysters. The final viral loads in oysters after 12 days remained under the detection limit (10 copies/g digestive gland) of the real-time RT-PCR. This reduction trend showed two-phase removal kinetics, with an initial slow reduction or slight increase in viruses during the first 2 days of depuration and subsequent stabilization with 0.12 to 2.64 log unit norovirus copies/g digestive gland per 2 days of depuration for the remaining time.
Norovirus;Crassostrea gigas;Oyster;Translocation;Depuration;
 Cited by
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