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Screening and Purification of an Antimicrobial Peptide from the Gill of the Manila Clam Ruditapes philippinarum
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 Title & Authors
Screening and Purification of an Antimicrobial Peptide from the Gill of the Manila Clam Ruditapes philippinarum
Seo, Jung-Kil;
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This study screened the biological activity of an acidified gill extract of the Manila clam Ruditapes philippinarum including antimicrobial, hemolytic, membrane permeabilization, and DNA-binding activity, and purified the antimicrobial material. The acidified gill extract showed potent antimicrobial activity against Bacillus subtilis and Escherichia coli without significant hemolytic activity, but showed no membrane permeabilization or DNA-binding ability. An antimicrobial material was purified from the acidified gill extract using C18 reversed-phase and cation-exchange high-performance liquid chromatography (HPLC). Treatment of the purified material with trypsin completely abolished all of the antibacterial activity against Bacillus subtilis, suggesting that the purified material is a proteinaceous antibiotic. The molecular weight of the purified material was 2571.9 Da, but no primary structural information was obtained due to N-terminal blocking. A future study should confirm the primary structure. Our results suggest that the Manila clam gill contains proteinaceous antibiotics that have a role in first-line defense. This information could be used to better understand the Manila clam innate immune system.
Manila clam;Ruditapes philippinarum;Antimicrobial peptide;Innate defense;Purification;
 Cited by
참담치(Mytilus coruscus) 유래 항균 펩타이드 분리 및 정제,오륜경;이민정;김영옥;남보혜;공희정;김주원;안철민;김동균;

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