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The Effects of tDCS and Montoya Stair Task on Sensorimotor Recovery and GFAP Expression in MCAo induced Stroke Rat Model
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 Title & Authors
The Effects of tDCS and Montoya Stair Task on Sensorimotor Recovery and GFAP Expression in MCAo induced Stroke Rat Model
Sim, Ki-Cheol; Kim, Gi-Do; Kim, Kyung-Yoon; An, Ho-Jung; Lee, Joon-Hee; Min, Kyoung-Ok; Kim, Gye-Yeop;
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This study is intended to examine the tDCS and Montoya stair task(MST) on sensorimotor recovery and glial scar expression in MCAo induced stroke model of rat. To achieve this goal, this study selected 80 SD rats of 8 weeks. The experiment groups were divided them into four groups, and assigned 20 rats to each group. Group I was a experimental control group; GroupII was a tDCS application group after MCAo; Group III was a MST application group after MCAo; Group IV was a tDCS and MST application group after MCAo. In each group, neurological function test measurement, motor behavior test, montoya stair task test, immunohistochemistric finding of GFAP expression finding were analyzed. In motor behavior test, the outcome of group I was significantly difference than the other group, especially from 14days. In montoya stair task test, the outcome of group I was significantly lower than the other group especially, group II were significantly different on 14days and group IV was most significantly difference than the other group. In immunohistochemistric finding, group II, III, IV were decrease GFAP expression on depend on time stream. These results throughout the MCAo due to focal ischemic brain injury rat model four weeks tDCS and MST was applied, when the neurobehavioural, upper extremity function and ability, histopathologic data suggest that sensorimotor function recovery and a positive influence on glial scar decrease and confirmed that.
MCAo;tDCS;Montoya Stair Task;GFAP;
 Cited by
Warlow C, Sudlow C, Dennis M, Wardlaw J, Sandercock P. stroke. Lancet 2003; 362(9391): 1211-1224. crossref(new window)

Widespread and long-lasting alterations in GABA(A)-receptor subtypes after focal cortical infarcts in rats: mediation by NMDA-dependent processes. J Cereb Blood Flow Metab 2002; 22(12): 1463-1475. crossref(new window)

Reinecke S, Lutzenburg M, Hagemann G, Bruehl C, Neumann-Haefelin T, Witte OW. Electrophy siological transcortical diaschisis after middle cerebral artery occlusion (MCAO) in rats. Neurosci Lett 1999; 12;261(1-2): 85-88.

Ward NS, Cohen LG. Mechanisms underlying recovery of motor function after stroke. Arch Neurol 2004; 61(12): 1844-1848. crossref(new window)

Boggio PS, Castro LO, Savagim EA, Braite R, Cruz VC, Rocha RR, Rigonatti SP, Silva MT, Fregni F. Enhancement of non-dominant hand motor function by anodal transcranial direct current stimulation. Neurosci Lett 2006; 404(1-2): 232-236. crossref(new window)

Montoya CP, Campbell-Hope LJ, Pemberton KD, Dunnett SB. The "staircase test": a measure of independent forelimb reaching and grasping abilities in rats. J Neurosci Methods 1991; 36(2-3): 219-228. crossref(new window)

Steiner J, Bernstein HG, Bielau H, Berndt A, Brisch R, Mawrin C, Keilhoff G, Bogerts B. Evidence for a wide extra-astrocytic distribution of S100B in human brain. BMC Neurosci 2007; 2: 8-20.

Eng LF, Lee YL, Yu AC, Fu WY. Gene expression in astrocytes during and after ischemia. Prog Brain Res 1995; 105: 245-253. crossref(new window)

Reymond I, Almarghini K, Tappaz M. Immunocy tochemical localization of cysteine sulfinate decarboxylase in astrocytes in the cerebellum and hippocampus: a quantitative double immunofluorescence study with glial fibrillary acidic protein and S-100 protein. Neuroscience 1996; 75(2): 619- 633. crossref(new window)

Ridet JL, Alonso G, Chauvet N, Chapron J, Koenig J, Privat A. Immunocytochemical characterization of a new marker of fibrous and reactive astrocytes. Cell Tissue Res 1996; 283(1): 39-49.

Matsui T, Tateishi N, Mori T, Kagamiishi Y, Satoh S, Katsube N, Morikawa E, Morimoto T, Asano T. Astrocytic activation and delayed infarct expansion after permanent focal ischemia in rats. Part II: suppression of astrocytic activation by a novel agent (R)-2-propyloctanoic acid(ONO-2506) leads to mitigation of delayed infarct expansion and early improvement of neurologic deficits. J Cereb Blood Flow Metab 2002; 22(6): 723-734. crossref(new window)

Yasuda Y, Tateishi N, Shimoda T, Satoh S, Ogitani E, Fujita S. Relationship between S100beta and GFAP expression in astrocytes during infarction and glial scar formation after mild transient ischemia. Brain Res 2004; 1021(1): 20-31. crossref(new window)

Fitch MT, Silver J. CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure. Exp Neurol 2008; 209(2): 294-301. crossref(new window)

Yiu G, He Z. Glial inhibition of CNS axon regeneration. Nat Rev Neurosci 2006; 7(8): 617-627. crossref(new window)

Li GZ, Zhong D, Yang LM, Sun B, Zhong ZH, Yin YH, Cheng J, Yan BB, Li HL. Expression of interleukin- 17 in ischemic brain tissue. Scand J Immunol 2005; 62(5): 481-486. crossref(new window)

Nagasawa H, Kogure K. Correlation between cerebral blood flow and histologic changes in a new rat model of middle cerebral artery occlusion. Stroke 1989; 20(8): 1037-1043. crossref(new window)

Kim SJ. Mechanism of motor recovery induced by repeated transcranial direct current stimulation in stroke rat model. Seoul university 2009; Doctoral thesis.

Bederson JB, Germano IM, Guarino L. Cortical blood flow and cerebral perfusion pressure in a new noncraniotomy model of subarachnoid hemorrhage in the rat. Stroke 1995; 26: 1086-1091. crossref(new window)

Garcia JH, Wagner S, Liu KF, Hu XJ. Neurological deficit and extent of neuronal necrosis attributable to middle cerebral artery occlusion in rats. Statistical validation. Stroke 1995; 26: 627-634. crossref(new window)

Nitsche MA, Cohen LG, Wassermann EM, Priori A, Lang N, Antal A, Paulus W, Hummel F, Boggio PS, Fregni F, Pascual-Leone A. Transcranial direct current stimulation: State of the art 2008. Brain Stimul 2008; 1(3): 206-223. crossref(new window)

Nitsche MA, Liebetanz D, Lang N, Antal A, Tergau F, Paulus W. Safety criteria for transcranial direct current stimulation (tDCS) in humans. Clin Neurophysiol 2003; 114(11): 2220-2222. crossref(new window)

Liebetanz D, Nitsche MA, Tergau F, Paulus W. Pharmacological approach to the mechanisms of transcranial DC-stimulation-induced aftereffects of human motor cortex excitability. Brain 2002; 125(10): 2238-2247. crossref(new window)

Nitsche MA, Paulus W. Sustained excitability elevations induced by transcranial DC motor cortex stimulation in humans. Neurology 2001; 27; 57(10): 1899-1901.

Greenough WT, Weiler IJ, Wang X. Synapseactivated protein synthesis as a possible mechanism of plastic neural change. Prog Brain Res 1994; 100: 189-194. crossref(new window)

Kloth V, Timmer M, Muller-Ostermeyer F, Winkler C, Grothe C, Nikkhah G. Enhanced survival, reinnervation, and functional recovery of intrastriatal dopamine grafts co-transplanted with Schwann cells overexpressing high molecular weight FGF-2 isoforms. Exp Neurol 2004; 187(1): 118-36. crossref(new window)

Berry SC, Anis NA, Burton NR, Lodge D. The dissociative anaesthetics, ketamine and phencyclidine, selectively reduce excitation of central mammalian neurones by N-methyl-aspartate. Br J Pharmacol 1983; 79(2): 565-575. crossref(new window)

Eng LF, Ghirnikar RS, Lee YL. lial fibrillary acidic protein: GFAP-thirty-one years (1969- 2000). Neurochem Res 2000; 25(9-10): 1439-1451. crossref(new window)