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Antibiotics Resistance and Molecular Analysis of Enterococcus Isolated from the Han-river in Korea
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  • Journal title : The Korean Journal of Microbiology
  • Volume 48, Issue 2,  2012, pp.116-124
  • Publisher : The Microbiological Society of Korea
  • DOI : 10.7845/kjm.2012.48.2.116
 Title & Authors
Antibiotics Resistance and Molecular Analysis of Enterococcus Isolated from the Han-river in Korea
Kwon, Oh-Min; Kim, Mal-Nam;
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Identification was performed in March 2008 for the 76 Enterococcus strains isolated from the Han-river, which is used as water supply for Seoul citizens. The antibiotic susceptibility, antibiotic resistant structural analysis, trans-conjugation, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were also carried out for the isolated strains. Among the isolated strains, 25 strains were E. casseliflavus, 4 strains were E. faecalis and 1 strain was E. hirae. Investigation of antibiotic susceptibility indicated that 15 strains demonstrated tolerance against vancomycin, and that 11 strains of E. faecium and 4 strains of E. casseliflavus were VRE. The vanA gene detection of the VRE strains revealed that 6 E. faecium strains were vancomycin-resistant Enterococcus faecium (VREF) possessing vanA. Analyses of transposon Tn1546 structure containing vanA demonstrated that Km36 and Km37 belonged to Tn1 type, Km20 and Km38 was Tn2 type, and Km39 and Km40 was Tn3 type. PFGE disclosed that among the 6 VREF strains, Km36 and Km37 exhibited equivalent subtype, while the rest 4 strains showed subtypes different to each other. MLST for the 6 VREF strains disclosed that 3 strains were ST78, while the rest 3 strains were ST18, ST192 and ST230, respectively. All these clonal complexes were derived from CC17 which has been isolated from clinical sources. 4 strains belonged to CC78, while the rest 2 strains were CC18 and CC192, respectively.
Enterococcus;Tn1546;vanA;multilocus sequence typing;vancomycin;
 Cited by
축산물과 수산물에서 분리된 장구균의 항생제 감수성 및 유전형 분석,김윤정;오미현;김용훈;김순한;박건상;주인선;

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