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Participation of protein disulfide isomerase 2 in the tolerance against mercury toxicity in Schizosaccharomyces pombe
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 Title & Authors
Participation of protein disulfide isomerase 2 in the tolerance against mercury toxicity in Schizosaccharomyces pombe
Choi, Jiye; Lim, Chang-Jin; Kim, Kyunghoon;
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The present work was undertaken to address the role of protein disulfide isomerase 2 (Pdi2) in the mercury-tolerance of Schizosaccharomyces pombe, using the Pdi2-overexpressing recombinant plasmid pYPDI2 and the corresponding vector plasmid pRS316. When exposed to mercuric chloride, the PDI2 overepxression cells grew significantly better than the vector control cells. They revealed the lower levels of intracellular reactive oxygen species (ROS) and nitric oxide (NO), when incubated with mercuric chloride for 6 h, than the vector control cells. The PDI2 overepxression cells contained the higher levels of total glutathione (GSH) and superoxide dismutase (SOD) activity than the vector control cells, after 6 h of incubation in mercuric chloride. However, the PDI2 overepxression cells contained similar levels of glutathione peroxidase (GPx) activities, compared to those of the vector control cells. Taken together, the S. pombe Pdi2 promotes the tolerance against mercury toxicity through up-regulating total GSH and SOD and subsequently attenuating ROS and NO elevations.
Schizosaccharomyces pombe;glutathione;mercury;protein disulfide isomerase;reactive oxygen species;superoxide dismutase;
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Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72, 248-254. crossref(new window)

De, A.P.A.M., Verissimo-Filho, S., Guimaraes, L.L., Silva, A.C., Takiuti, J.T., and Lopes, L.R. 2011. Protein disulfide isomerase redox-dependent association with p47 (phox): evidence for an organizer role in leukocyte NADPH oxidase activation. J. Leukoc. Biol. 90, 799-810. crossref(new window)

Genestra, M. 2007. Oxyl radicals, redox-sensitive signalling cascades and antioxidants. Cell. Signal. 19, 1807-1819. crossref(new window)

Gilbert, H.F. 1997. Protein disulfide isomerase and assisted protein folding. J. Biol. Chem. 272, 29399-29402. crossref(new window)

Gueldry, O., Lazard, M., Delort, F., Dauplais, M., Grigoras, I., Blanquet, S., and Plateau, P. 2003. Ycf1p-dependent Hg(II) detoxification in Saccharomyces cerevisiae. Eur. J. Biochem. 270, 2486-2496. crossref(new window)

Jan, A.T., Ali, A., and Haq, Q. 2011. Glutathione as an antioxidant in inorganic mercury induced nephrotoxicity. J. Postgrad. Med. 57, 72-77. crossref(new window)

Jeenes, D.J., Pfaller, R., and Archer, D.B. 1997. Isolation and characterization of a novel stress-inducible PDI-family gene from Aspergillus niger. Gene 193, 151-156. crossref(new window)

Kiani-Esfahani, A., Tavalaee, M., Deemeh, M.R., Hamiditabar, M., and Nasr-Esfahani, M.H. 2012. DHR123: an alternative probe for assessment of ROS in human spermatozoa. Syst. Biol. Reprod. Med. 58, 168-174. crossref(new window)

Kig, C. and Temizkan, G. 2009. Nitric oxide as a signaling molecule in the fission yeast Schizosaccharomyces pombe. Protoplasma 238, 59-66. crossref(new window)

Kim, S.J., Choi, Y.S., Kim, H.G., Park, E.H., and Lim, C.J. 2006. Cloning, characterization and regulation of a protein disulfide isomerase from the fission yeast Schizosaccharomyces pombe. Mol. Biol. Rep. 33, 187-196. crossref(new window)

Lee, E.H., Hyun, D.H., Park, E.H., and Lim, C.J. 2010. A second protein disulfide isomerase plays a protective role against nitrosative and nutritional stresses in Schizosaccharomyces pombe. Mol. Biol. Rep. 37, 3663-3671. crossref(new window)

Lee, Y.Y., Kim, H.G., Jung, H.I., Shin, Y.H., Hong, S.M., Park, E.H., Sa, J.H., and Lim, C.J. 2002. Activities of antioxidant and redox enzymes in human normal hepatic and hepatoma cell lines. Mol. Cells 14, 305-311.

Lenartova, V., Holovska, K., and Javorsky, P. 1998. The influence of mercury on the antioxidant enzyme activity of rumen bacteria Streptococcus bovis and Selenomonas ruminantium. FEMS Microbiol. Ecol. 27, 319-325. crossref(new window)

Lu, D.P. and Christopher, D.A. 2008. Endoplasmic reticulum stress activates the expression of a sub-group of protein disulfide isomerase genes and AtbZIP60 modulates the response in Arabidopsis thaliana. Mol. Genet. Genomics 280, 199-210. crossref(new window)

Lund, B.O., Miller, D.M., and Woods, J.S. 1991. Mercury-induced $H_2O_2$ production and lipid peroxidation in vitro in rat kidney mitochondria. Biochem. Pharmacol. 42 Suppl, S181-S187. crossref(new window)

Nakagawa, K., Saijo, N., Tsuchida, S., Sakai, M., Tsunokawa, Y., Yokota, J., Muramatsu, M., Sato, K., Terada, M., and Tew, K.D. 1990. Glutathione-S-transferase pi as a determinant of drug resistance in transfectant cell lines. J. Biol. Chem. 265, 4296-4301.

Royall, J.A. and Ischiropoulos, H. 1993. Evaluation of 2',7'-dichlorofluorescin and dihydrorhodamine 123 as fluorescent probes for intracellular $H_2O_2$ in cultured endothelial cells. Arch. Biochem. Biophys. 302, 348-355. crossref(new window)

Saloheimo, M., Lund, M., and Penttila, M.E. 1999. The protein disulfide isomerase gene of the fungus Trichoderma reesei is induced by endoplasmic reticulum stress and regulated by the carbon source. Mol. Gen. Genet. 262, 35-45. crossref(new window)

Sherman, M.P., Aeberhard, E.E., Wong, V.Z., Griscavage, J.M., and Ignarro, L.J. 1993. Pyrrolidine dithiocarbamate inhibits induction of nitric oxide synthase activity in rat alveolar macrophages. Biochem. Biophys. Res. Commun. 191, 1301-1308. crossref(new window)

Sorensen, B.S., Horsman, M.R., Vorum, H., Honore, B., Overgaard, J., and Alsner, J. 2009. Proteins upregulated by mild and severe hypoxia in squamous cell carcinomas in vitro identified by proteomics. Radiother. Oncol. 92, 443-449. crossref(new window)

Tanaka, S., Uehara, T., and Nomura, Y. 2000. Up-regulation of protein-disulfide isomerase in response to hypoxia/brain ischemia and its protective effect against apoptotic cell death. J. Biol. Chem. 275, 10388-10393. crossref(new window)

Tian, G., Kober, F.X., Lewandrowski, U., Sickmann, A., Lennarz, W.J., and Schindelin, H. 2008. The catalytic activity of protein-disulfide isomerase requires a conformationally flexible molecule. J. Biol. Chem. 283, 33630-33640. crossref(new window)

Vatamaniuk, O.K., Bucher, E.A., Ward, J.T., and Rea, P.A. 2001. A new pathway for heavy metal detoxification in animals. Phytochelatin synthase is required for cadmium tolerance in Caenorhabditis elegans. J. Biol. Chem. 276, 20817-20820. crossref(new window)

Westwater, J., McLaren, N.F., Dormer, U.H., and Jamieson, D.J. 2002. The adaptive response of Saccharomyces cerevisiae to mercury exposure. Yeast 19, 233-239. crossref(new window)

Whittaker, S.G., Smith, D.G., Foster, J.R., and Rowland, I.R. 1990. Cytochemical localization of mercury in Saccharomyces cerevisiae treated with mercuric chloride. J. Histochem. Cytochem. 38, 823-827. crossref(new window)

Wilkins, B. and Gilbert, H.F. 2004. Protein disulfide isomerase. Biochem. Biophys. Res. Commun. 1699, 35-44.

Zhang, H., He, J., Ji, Y., Kato, A., and Song, Y. 2008. The effect of calnexin deletion on the expression level of PDI in Saccharomyces cerevisiae under heat stress conditions. Cell. Mol. Biol. Lett. 13, 38-48.