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Isolation and characterization of a Bacillus spp. for manufacturing the feed additives in livestock
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 Title & Authors
Isolation and characterization of a Bacillus spp. for manufacturing the feed additives in livestock
Park, Hae Suk; Jo, Seung Wha; Yim, Eun Jung; Kim, Yun Sun; Moon, Sung Hyun; Cho, Ho Seong; Kim, Hyun-Young; Cho, Yong Sik; Cho, Sung Ho;
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The aims of this study were to isolate spore-forming Bacillus strains that exhibit high digestibility and anti-pathogenic bacteria toward feed for calves. Total 136 spore-forming strains were isolated from finished feeds and their ingredients. Among them, 93 strains were identified as Bacillus species when analyzed by 16S rRNA sequencing. For industrial use, three strains named as Bacillus licheniformis SHS14, B. subtilis LCB7, B. amyloliquefaciens LCB10 were selected after evaluating the industrial standards that are related with heat and acid resistance, enzyme activities, and anti-pathogenic activities against Samonella dublin ATCC15480 and E. coli K99. After each culture, 3 selected strains were mixed together at 1:1:1 (v/v/v) ratio and then prepared as the mixed starter culture for feeding. The changes in microbial community were analyzed via 16S rRNA metagenomics. The initial community ratio among three strains was maintained even after manufacturing into final products. Also, in vitro, enzymatic and anti-pathogenic activities were almost same as those when cultured in single culture, and results of anti-pathogenic activities conducted with calves showed 90% activities against lincomycin, which would be indicative of a promising feed starter.
Bacillus;fermented feed;spore forming bacteria;starter culture;
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Banihashemi, A., Dyke, M., and Huck, P. 2012. Long-amplicon propidium monoazide - PCR enumeration assay to detect viable Campylobacter and Salmonella. J. Appl. Microbiol. 113, 863-873. crossref(new window)

De Clerck, E., Gevers, D., De Ridder, K., and De Vos, P. 2004. Screening of bacterial contamination during gelatin production by means of denaturing gradient gel electrophoresis, focused on Bacillus and related endospore-forming genera. J. Appl. Microbiol. 96, 1333-1341. crossref(new window)

EFSA Panel on Biological Hazards (BIOHAZ). 2010. Scientific opinion on the maintenance of the list of QPS biological agents intentionally added to food and feed. EFSA J. 8, 1944.

Kang, M.I., Han, D.U., Chung, Y.U., Chung, D.Y., Lee, C.Y., Lee, C.G., Wee, S.H., and Cho, J.J. 2001. Survey on Korean-native calves diseases and mortality. Korean J. Vet. Serv. 24, 223-241.

Kim, J.W., Jun, K.D., Kang, J.S., Jang, J.S., Ha, B.J., and Lee, J.H. 2005. Characterization of Bacillus licheniformis as a probiotic. Korean J. Biotechnol. Bioeng. 20, 359-362.

Kim, K.H. and Song, M.K. 2013. Effects of feeding level of feedstuffs fermented with complex probiotics on growth of Holstein male calves. Korean Bull. Animal Biotechnol. 5, 1-9.

Larsen, N., Thorsen, L., Kpikpi, E.N., Lauridsen, B.S., Cantor, M.D., Nielsen, B., Brockmann, E., Derkx, P.M.F., and Jespersen, L. 2014. Characterization of Bacillus spp. strains for use as probiotic additives in pig feed. Appl. Microbiol. Biotechnol. 98, 1105-1118. crossref(new window)

Leser, T.D., Knarreborg, A., and Worm, J. 2008. Germination and outgrowth of Bacillus subtilis and Bacillus licheniformis spores in the gastrointestinal tract of pigs. J. Appl. Microbiol. 104, 1025-1033. crossref(new window)

Paik, H.D., Jung, M.Y., Jung, H.Y., Kim, W.S., and Kim, K.T. 2002. Characterization of Bacillus polyfermenticus SCD for oral bacteriotherapy of gastrointestinal disorders. Korean J. Food. Sci. Technol. 34, 73-78.

Sanders, M.E., Morelli, L., and Tompkins, A. 2003. Sporeformers as human probiotics: Bacillus, Sporolactobacillus, and Brevibacillus. Comp. Rev. Food Sci. Food Safety 2, 101-110. crossref(new window)

Shin, T.S. 2014. Development of supplementary fodder as an alternative of antibiotics using by heat- resistance microbes. Busan National University.

Szczawinska, M.E., Thayer, D.W., and Phillips, J.G. 1991. Fate of unirradiated Salmonella in irradiated mechanically deboned chicken meat. Int. J. Food Microbiol. 14, 313-324. crossref(new window)

Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., and Kumar, S. 2011. MEGA5 : Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol. Biol. Cell 28, 2731-2739.

Veith, B., Herzberg, C., Steckel, S., Feesche, J., Maurer, K.H., Ehrenreich, P., Baumer, S., Henne, A., Liesegang, H., Merkl, R., et al. 2004. The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential. J. Mol. Microbiol. Biotechnol. 7, 204-211. crossref(new window)

Yousif, A.A., Mahmood, N.M., and Al-Taai, N.A. 2013. Immunization of mice with killed E. coli K99 vaccine for protection against colibaccillosis. Int. J. Microbiol. Res. 5, 482-485. crossref(new window)