Advanced SearchSearch Tips
Effects of fission yeast ortholog of THOC5 on growth and mRNA export in fission yeast
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Effects of fission yeast ortholog of THOC5 on growth and mRNA export in fission yeast
Koh, Eun-Jin; Yoon, Jin Ho;
  PDF(new window)
THO/TREX complex plays an important role in transcriptional elongation, mRNA processing, nuclear RNA export, and genome stability. A fission yeast, Schizosaccharomyces pombe, SPBC577.04 gene encoding the ortholog of THOC5, a component of THO/TREX complex, was identified and characterized. The S. pombe thoc5 (spthoc5) is not essential for both growth and mRNA export, but deletion of the spthoc5 gene caused growth defect and slight accumulation of RNA in the nucleus. And the functional spThoc5-GFP protein is localized mainly in the nucleus. Co-immunoprecipitation analysis showed that the Hpr1(THOC1) protein, an evolutionally well-conserved component of THO/TREX complex, interacted with spThoc5 as well as Tho2(THOC2), another subunit of THO complex. These results suggest that S. pombe Thoc5 as a component of THO/TREX complex is also involved in mRNA export from the nucleus.
S. pombe;mRNA export;THOC5;THO/TREX complex;
 Cited by
Abruzzi, K.C., Lacadie, S., and Rosbash, M. 2004. Biochemical analysis of TREX complex recruitment to intronless and intron-containing yeast genes. EMBO J. 23, 2620-2631. crossref(new window)

Aguilera, A. 2002. The connection between transcription and genomic instability. EMBO J. 21, 195-201. crossref(new window)

Alfa, C., Fantes, P., Hyams, J., Mcleod, M., and Warbrick, E. 1993. Experiments with Fission Yeast. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, USA.

Chanarat, S., Seizl, M., and Strasser, K. 2011. The Prp19 complex is a novel transcription elongation factor required for TREX occupancy at transcribed genes. Genes Dev. 25, 1147-1158. crossref(new window)

Chavez, S., Beilharz, T., Rondon, A.G., Erdjument-Bromage, H., Tempst, P., Svejstrup, J.Q., Lithgow, T., and Aguilera, A. 2000. A protein complex containing Tho2, Hpr1, Mft1 and a novel protein, Thp2, connects transcription elongation with mitotic recombination in Saccharomyces cerevisiae. EMBO J. 19, 5824-5834. crossref(new window)

Cheng, H., Dufu, K., Lee, C.S., Hsu, J.L., Dias, A., and Reed, R. 2006. Human mRNA export machinery recruited to the 5' end of mRNA. Cell 127, 1389-1400. crossref(new window)

Dominguez-Sanchez, M.S., Barroso, S., Gomez-Gonzalez, B., Luna, R., and Aguilera, A. 2011. Genome instability and transcription elongation impairment in human cells depleted of THO/TREX. PLoS Genet. 7, e1002386. crossref(new window)

Forsburg, S.L. and Sherman, D.A. 1997. General purpose tagging vectors for fission yeast. Gene 191, 191-195. crossref(new window)

Gould, K.L., Ren, L., Feoktistova, A.S., Jennings, J.L., and Link, A.J. 2004. Tandem affinity purification and identification of protein complex components. Methods 33, 239-244. crossref(new window)

Guria, A., Tran, D.D., Ramachandran, S., Koch, A., El Bounkari, O., Dutta, P., Hauser, H., and Tamura, T. 2011. Identification of mRNAs that are spliced but not exported to the cytoplasm in the absence of THOC5 in mouse embryo fibroblasts. RNA 17, 1048-1056. crossref(new window)

Huertas, P. and Aguilera, A. 2003. Cotranscriptionally formed DNA: RNA hybrids mediate transcription elongation impairment and transcription-associated recombination. Mol. Cell 12, 711-721. crossref(new window)

Hurt, E., Luo, M.J., Rother, S., Reed, R., and Strasser, K. 2004. Cotranscriptional recruitment of the serine-arginine-rich (SR)-like proteins Gbp2 and Hrb1 to nascent mRNA via the TREX complex. Proc. Natl. Acad. Sci. USA 101, 1858-1862. crossref(new window)

Jimeno, S., Rondon, A.G., Luna, R., and Aguilera, A. 2002. The yeast THO complex and mRNA export factors link RNA metabolism with transcription and genome instability. EMBO J. 21, 3526-3535. crossref(new window)

Johnson, S.A., Kim, H., Erickson, B., and Bentley, D.L. 2011. The export factor Yra1 modulates mRNA 3' end processing. Nat. Struct. Mol. Biol. 18, 1164-1171. crossref(new window)

Katahira, J., Inoue, H., Hurt, E., and Yoneda, Y. 2009. Adaptor Aly and co-adaptor Thoc5 function in the Tap-p15-mediated nuclear export of HSP70 mRNA. EMBO J. 28, 556-567. crossref(new window)

Koh, E. and Yoon, J.H. 2014. Effects of spThoc7 deletion on growth and mRNA export in fission yeast. Korean J. Microbiol. 50, 249-253. crossref(new window)

Koh, E. and Yoon, J.H. 2015. Effects of Tho2, a component of THO complex, on growth and mRNA export in fission yeast. Korean J. Microbiol. 51, 181-185. crossref(new window)

Lee, H. and Yoon, J.H. 2012. Effects of the repression of sphpr1 expression on growth and mRNA export in fission yeast. Korean J. Microbiol. 48, 171-174. crossref(new window)

Li, Y., Wang, X., Zhang, X., and Goodrich, D.W. 2005. Human hHpr1/p84/Thoc1 regulates transcriptional elongation and physically links RNA polymerase II and RNA processing factors. Mol. Cell. Biol. 25, 4023-4033. crossref(new window)

Luna, R., Rondon, A.G., and Aguilera, A. 2012. New clues to understand the role of THO and other functionally related factors in mRNP biogenesis. Biochim. Biophys. Acta. 1819, 514-520. crossref(new window)

Masuda, S., Das, R., Cheng, H., Hurt, E., Dorman, N., and Reed, R. 2005. Recruitment of the human TREX complex to mRNA during splicing. Genes Dev. 19, 1512-1517. crossref(new window)

Meinel, D.M., Burkert-Kautzsch, C., Kieser, A., O'Duibhir, E., Siebert, M., Mayer, A., Cramer, P., Soding, J., Holstege, F.C., and Strasser, K. 2013. Recruitment of TREX to the transcription machinery by its direct binding to the phospho-CTD of RNA polymerase II. PLoS Genet. 9, e1003914. crossref(new window)

Moreno, S., Klar, A., and Nurse, P. 1991. Molecular genetic analysis of fission yeast Schizosaccharomyces pombe. Methods Enzymol. 194, 795-823. crossref(new window)

Pena, A., Gewartowski, K., Mroczek, S., Cuellar, J., Szykowska, A., Prokop, A., Czarnocki-Cieciura, M., Piwowarski, J., Tous, C., Aguilera, A., et al. 2012. Architecture and nucleic acids recognition mechanism of the THO complex, an mRNP assembly factor. EMBO J. 31, 1605-1616. crossref(new window)

Perales, R. and Bentley, D. 2009. "Cotranscriptionality": the transcription elongation complex as a nexus for nuclear transactions. Mol. Cell 36, 178-191. crossref(new window)

Puig, O., Caspary, F., Rigaut, G., Rutz, B., Bouveret, E., Bragado-Nilsson, E., Wilm, M., and Seraphin, B. 2001. The tandem affinity purification (TAP) method: a general procedure of protein complex purification. Methods 24, 218-229. crossref(new window)

Rehwinkel, J., Herold, A., Gari, K., Kocher, T., Rode, M., Ciccarelli, F.L., Wilm, M., and Izaurralde, E. 2004. Genome-wide analysis of mRNAs regulated by the THO complex in Drosophila melanogaster. Nat. Struct. Mol. Biol. 11, 558-566. crossref(new window)

Rigaut, G., Shevchenko, A., Rutz, B., Wilm, M., Mann, M., and Seraphin, B. 1999. A generic protein purification method for protein complex characterization and proteome exploration. Nat. Biotechnol. 17, 1030-1032. crossref(new window)

Rougemaille, M., Dieppois, G., Kisseleva-Romanova, E., Gudipati, R.K., Lemoine, S., Blugeon, C., Boulay, J., Jensen, T.H., Stutz, F., Devaux, F., et al. 2008. THO/Sub2p functions to coordinate 3'-end processing with gene-nuclear pore association. Cell 135, 308-321. crossref(new window)

Stewart, M. 2010. Nuclear export of mRNA. Trends Biochem. Sci. 35, 609-617. crossref(new window)

Stirling, P.C., Chan, Y.A., Minaker, S.W., Aristizabal, M.J., Barrett, I., Sipahimalani, P., Kobor, M.S., and Hieter, P. 2012. R-loop-mediated genome instability in mRNA cleavage and polyadenylation mutants. Genes Dev. 26, 163-175. crossref(new window)

Strasser, K., Masuda, S., Mason, P., Pfannstiel, J., Oppizzi, M., Rodriguez-Navarro, S., Rondon, A.G., Aguilera, A., Struhl, K., Reed, R., et al. 2002. TREX is a conserved complex coupling transcription with messenger RNA export. Nature 417, 304-308. crossref(new window)

Voynov, V., Verstrepen, K.J., Jansen, A., Runner, V.M., Buratowski, S., and Fink, G.R. 2006. Genes with internal repeats require the THO complex for transcription. Proc. Natl. Acad. Sci. USA 103, 14423-14428. crossref(new window)

Yoon, J.H., Love, D., Guhathakurta, A., Hanover, J.A., and Dhar, R. 2000. Mex67p of Schizosaccharomyces pombe interacts with Rae1p in mediating mRNA export. Mol. Cell. Biol. 20, 8767-8782. crossref(new window)

Zenklusen, D., Vinciguerra, P., Wyss, J.C., and Stutz, F. 2002. Stable mRNP formation and export require cotranscriptional recruitment of the mRNA export factors Yra1p and Sub2p by Hpr1p. Mol. Cell. Biol. 22, 8241-8253. crossref(new window)