Advanced SearchSearch Tips
Construction of Pseudoalteromonas - Escherichia coli shuttle vector based on a small plasmid from the marine organism Pseudoalteromonas
facebook(new window)  Pirnt(new window) E-mail(new window) Excel Download
 Title & Authors
Construction of Pseudoalteromonas - Escherichia coli shuttle vector based on a small plasmid from the marine organism Pseudoalteromonas
Kim, Dockyu; Park, Ha Ju; Park, Hyun;
  PDF(new window)
A small plasmid (pDK4) from the Antarctic marine organism Pseudoalteromonas sp. PAMC 21150, was purified, sequenced and analyzed. pDK4 was determined to be 3,480 bp in length with a G+C content of 41.64% and contains three open reading frames encoding a replication initiation protein (RepA), a conjugative mobilization protein (Mob) and a hypothetical protein. PCR-amplified pDK4 was cloned in high-copy pUC19 to yield the fusion vector pDOC153. The chloramphenicol resistance gene was inserted into pDOC153 to give an ampicillin and chloramphenicol-resistant, Pseudoalteromonas - Escherichia coli shuttle vector (7,216 bp; pDOC155). The TonB-dependent receptor (chi22718_IV ) and exochitinase (chi22718_III ) genes from Arctic marine P. issachenkonii PAMC 22718 were cloned into pDOC155 to produce pDOC158 and pDOC165, respectively. Both vector derivatives were transferred into plasmid-free Pseudoalteromonas sp. PAMC 22137 by the triparental mating method. PCR experiments showed that the genes were stably maintained both in Pseudoalteromonas sp. PAMC 22137 and E. coli cells, indicating the potential use of pDOC155 as a new gene transfer system into marine Pseudoalteromonas spp.
Pseudoalteromonas;cold-active enzyme;gene transfer;shuttle vector;
 Cited by
Al Khudary, R., Stosser, N.I., Qoura, F., and Antranikian, G. 2008. Pseudoalteromonas arctica sp. nov., an aerobic, psychrotolerant, marine bacterium isolated from Spitzbergen. Int. J. Syst. Evol. Microbiol. 58, 2018-2024. crossref(new window)

Feller, G., d'Amico, D., and Gerday, C. 1999. Thermodynamic stability of a cold-active alpha-amylase from the Antarctic bacterium Alteromonas haloplanctis. Biochemistry 38, 4613-4619. crossref(new window)

Kim, D., Park, H.J., Kim, I.C., and Yim, J.H. 2014. A new approach for discovering cold-active enzymes in a cell mixture of pure-cultured bacteria. Biotechnol. Lett. 36, 567-573. crossref(new window)

Mancuso Nichols, C.A., Garon, S., Bowman, J.P., Raguenes, G., and Guezennec, J. 2004. Production of exopolysaccharides by Antarctic marine bacterial isolates. J. Appl. Microbiol. 96, 1057-1066. crossref(new window)

Marx, J.C., Collins, T., D'Amico, S., Feller, G., and Gerday, C. 2007. Cold-adapted enzymes from marine Antarctic microorganisms. Mar. Biotechnol. 9, 293-304. crossref(new window)

Medigue, C., Krin, E., Pascal, G., Barbe, V., Bernsel, A., Bertin, P.N., Cheung, F., Cruveiller, S., D'Amico, S., Duilio, A., et al. 2005. Coping with cold: the genome of the versatile marine Antarctica bacterium Pseudoalteromonas haloplanktis TAC125. Genome Res. 15, 1325-1335. crossref(new window)

Wang, P., Yu, Z., Li, B., Cai, X., Zeng, Z., Chen, X., and Wang, X. 2015. Development of an efficient conjugation-based genetic manipulation system for Pseudoalteromonas. Microb. Cell Fact. 14, 11. crossref(new window)

Wietz, M., Gram, L., Jorgensen, B., and Schramm, A. 2010. Latitudinal patterns in the abundance of major marine bacterioplankton groups. Aquat. Microb. Ecol. 61, 179-189. crossref(new window)

Yu, Z.C., Zhao, D.L., Ran, L.Y., Mi, Z.H., Wu, Z.Y., Pang, X., Zhang, X.Y., Su, H.N., Shi, M., Song, X.Y., et al. 2014. Development of a genetic system for the deep-sea psychrophilic bacterium Pseudoalteromonas sp. SM9913. Microb. Cell Fact. 13, 13. crossref(new window)

Zhao, D.L., Yu, Z.C., Li, P.Y., Wu, Z.Y., Chen, X.L., Shi, M., Yu, Y., Chen, B., Zhou, B.C., and Zhang, Y.Z. 2011. Characterization of a cryptic plasmid pSM429 and its application for heterologous expression in psychrophilic Pseudoalteromonas. Microb. Cell Fact. 10, 30. crossref(new window)